Succinic anhydride modified polymine grafting medium, preparation method and application thereof

The technology of polyethyleneimine and succinic anhydride is applied in the fields of succinic anhydride modified polyethyleneimine grafting medium and preparation, and protein chromatographic separation, and can solve the problems of reducing production cost, restricting the production of biological macromolecules, etc. The effect of good tolerance and broad application prospects

Inactive Publication Date: 2016-10-12
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the upstream processing of these biomacromolecules has made significant progress in product yield, the downstream bioprocessing and purification processes still constrain the production of biomacromolecules and need to be simplified and reduce production costs

Method used

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  • Succinic anhydride modified polymine grafting medium, preparation method and application thereof
  • Succinic anhydride modified polymine grafting medium, preparation method and application thereof
  • Succinic anhydride modified polymine grafting medium, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Introduce polyethyleneimine grafted chains on the surface of the agarose gel through the epoxy-based spacer arm, and then take 5 g of polyethyleneimine grafted media with an ion exchange capacity of 740 mmol / L and use N,N-dimethyl formazan Amide soaking until the solvent in the well is completely replaced by N,N-dimethylformamide;

[0028] 2) Drain the medium obtained in step 1) with a G3 funnel and place it in a 50mL Erlenmeyer flask, then continue to add 20mL N,N-dimethylformamide into the Erlenmeyer flask, mix well, and prepare a medium suspension;

[0029] 3) Add 0.111g of succinic anhydride to the medium suspension obtained in step 2), mix well, place in a constant temperature water bath at 25°C, react at 170rpm for 24h, and then rinse with N,N-dimethylformamide, ethanol and water in sequence As a medium, a succinic anhydride-modified polyethyleneimine grafted medium with a carboxyl ion exchange capacity of 150 mmol / L was obtained.

Embodiment 2

[0031] The amount of succinic anhydride added in Example 1 was changed to 0.2294 g, and under other conditions unchanged, a succinic anhydride-modified polyethyleneimine grafted medium with a carboxyl ion exchange capacity of 240 mmol / L was obtained.

Embodiment 3

[0033] The amount of succinic anhydride added in Example 1 was changed to 0.296g, and under other conditions unchanged, a succinic anhydride-modified polyethyleneimine grafted medium with a carboxyl ion exchange capacity of 270mmol / L was obtained.

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Abstract

The invention relates to a succinic anhydride modified polymine grafting medium, a preparation method and application thereof. The sepharose gel particle with average grain diameter of 50-170mu m is taken as a chromatography medium of the succinic anhydride modified polymine grafting medium; polymine grafting chain is introduced to the surface through an epoxy group spacer arm; the amino group on the polymine grafting chain is combined with succinic anhydride, so that the succinic anhydride modified polymine grafting medium with carboxyl ion exchange capacity being 150-970mmol / L is obtained. The succinic anhydride modified polymine grafting medium provided by the invention has higher adsorption rate to protein or strong adsorption property to protein, higher static adsorption capacity and excellent tolerance to salt concentration. The medium is conveniently cleaned and sterilized and is easy to regenerate; the biocompatibility is excellent; the preparation method is simple and the cost is low; the medium has wide application prospect in protein separation and purification.

Description

technical field [0001] The invention relates to a succinic anhydride-modified polyethyleneimine grafting medium, a preparation method and application thereof, and belongs to the protein chromatographic separation technology in the field of biotechnology. Background technique [0002] The development of genetic engineering and recombinant DNA technology has promoted the demand for recombinant or native proteins as well as biological macromolecules such as plasmid DNA and virus-like particles. For the biopharmaceutical industry, the core issue is to deliver these new therapeutic products to a demanding and regulated market. Although the upstream processing of these biomacromolecules has made significant progress in product yield, the downstream bioprocessing and purification processes still restrict the production of biomacromolecules and need to be simplified and reduce production costs. Chromatography, especially ion-exchange chromatography, is widely used in the downstream...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/286B01J20/30B01J39/18B01J39/26B01D15/36B01D15/32
CPCB01D15/32B01D15/362B01J20/286B01J39/26B01J2220/52B01J2220/80
Inventor 孙彦赵洋洋余林玲董晓燕
Owner TIANJIN UNIV
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