The invention discloses a novel pharmaceutical equipment leak detection device. The leak detection device comprises a device body, a roller is arranged at the bottom of the device body, a leakage detection instrument groove is embedded in the middle of the surface of the device body, a leak detector is arranged in the leak detector groove, a leak detector switch is arranged on the surface of the leak detector, a baffle valve and a filter screen are respectively fixed in the leak detector, a vacuum tube winding column is fixed to the top of the surface of the device body, a fixing column is fixed on one side of the top of the device body, the top of the fixing column is connected with a vacuum tube, helium is used as leakage indication gas, and the helium is light in mass and high in movement speed, and small in molecular diameter, and under the same condition, the linear movement distance is long. The accurate positioning, quantitation, and qualitation of the micro leakage gap of the pharmaceutical equipment can be realized, the sensitivity is extremely high, and the leakage rate and the position of the pharmaceutical equipment can be accurately measured, the performance is stable,the cost is low, and the efficiency is high due to the detection by a mass spectrum chamber.