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Method for distinguishing Apis cerana cerana honey from Apis mellifera ligustica honey

A honey, Italian bee technology, applied in the field of biochemistry, can solve the problems of brand image and enterprise loss, low production of honey in China Bee, and unfavorable development of the bee product market.

Active Publication Date: 2021-04-02
秦皇岛海关技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low production of Chinese bee honey, the market price is 3-10 times that of Italian bee honey. Driven by profit, there are also honey products that use Italian bee honey to pretend to be or be mixed with Chinese bee honey. As well as related losses to the brand image and enterprise of China Bee Honey, it has also triggered a crisis of trust among consumers, which is not conducive to the healthy development of the bee product market

Method used

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  • Method for distinguishing Apis cerana cerana honey from Apis mellifera ligustica honey
  • Method for distinguishing Apis cerana cerana honey from Apis mellifera ligustica honey
  • Method for distinguishing Apis cerana cerana honey from Apis mellifera ligustica honey

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Discovery of the key difference substances between bee honey and Italian bee honey in embodiment 1

[0073] Sample source:

[0074] A total of 50 common real honey samples were collected from beekeepers: 40 of which were Italian bee honey, including 10 acacia honey, 10 jujube honey, 10 wattle honey, and 10 linden honey; Chinese bee honey (earth honey) 10.

[0075] Experimental steps:

[0076] Weigh 2.0 g of the honey sample into a headspace bottle. Placed in an automated headspace solid-phase microextraction device to extract and enrich honey samples, the solid-phase microextraction fiber used was 30 μm PDMS, the extraction temperature was 60°C, the extraction time was 30 minutes, and the equilibration time was 5 minutes. The analysis time is 5 min; use GC-MS for detection.

[0077] The detection conditions of GC are:

[0078] Chromatographic column: DB-5MS capillary column;

[0079] Temperature program: initial temperature 50°C, keep for 1min; then rise to 250°C ...

Embodiment 2

[0099] The optimization of the identification method of bee honey and Italian bee honey in embodiment 2

[0100] Sample source is with embodiment 1.

[0101] The experimental conditions such as extraction temperature and extraction time were further optimized, and the quantitative method of matrix working curve was determined.

[0102] 1. Optimization of extraction temperature

[0103] Take 2g of Italian honey in a 20mL headspace bottle, and perform SPME extraction and GC-MS detection at 40, 50, 60, 70, and 80°C, respectively.

[0104] The experimental results showed that as the extraction temperature increased, the peak areas of the target C9T and C9P gradually increased, but when the temperature was higher than 70°C, the nutritional components of honey might change, and too high a temperature would cause fiber damage. However, the higher target response at 60 °C can meet the detection requirements, so the selected extraction temperature is 60 °C.

[0105] 2. Optimization ...

Embodiment 3

[0121] Embodiment 3 Actual sample detection and Chinese bee honey identification

[0122] Sample source:

[0123] Fifteen batches of honey samples identified as "earth honey" or "China bee honey" were purchased from offline supermarkets and online platforms.

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Abstract

The invention relates to a method for distinguishing distinguishing Apis cerana cerana honey from Apis mellifera ligustica honey. The method comprises the steps of (1) weighing a detected amount of honey sample to be detected; (2) detecting volatile hydrocarbon compounds in the honey to be detected by using HS-SPME-GC-MS to obtain a detection spectrogram, and quantifying Cis-9-Tricosene, (C9T), isomers thereof, namely, Iso-9-Tricosene, Cis-9-Pentacosene, (C9P) and an isomer Iso-9-Pentacosene in the honey sample to be detected according to the spectrogram; and (3) identifying the Apis cerana cerana honey and Apis mellifera ligustica honey according to the contents of C9T and C9P in the honey and the proportions of C9T and C9P to respective isomers.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for distinguishing Chinese bee honey and Italian bee honey. Background technique [0002] Honey is a natural sweet substance that is fully brewed by bees after collecting nectar or honeydew from nectar source plants and combining them with their own secretions. Honey has rich nutritional value and natural sweetness, and is favored by consumers. [0003] Italian bee (Apis mellifera ligustica, referred to as Italian bee) has a strong ability to produce honey, and is currently the main species of bee breeding in China. Italian bees are mainly raised in areas where the main pollen source plants are suitable for collecting honey powder distributed in large areas. source, mainly producing unifloral nectar. Apis cerana cerana (Apis cerana cerana, referred to as Apis cerana) is a unique subspecies in China. Apis cerana cerana is mainly raised in mountainous an...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/062
Inventor 崔宗岩纪涌彦柳吉芹纪欣欣黄学者张进杰
Owner 秦皇岛海关技术中心
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