Application of 3-amino-2-naphthoic acid in identification of apis cerana honey and apis mellifera honey

Abstract

The invention relates to the field of food detection, and particularly discloses an application of 3-amino-2-naphthoic acid in identification of apis cerana honey and apis mellifera honey. Meanwhile,the invention provides a method for identifying the apis cerana honey and the apis mellifera honey by taking the content of the 3-amino-2-naphthoic acid as an identification basis. The method comprises the steps of: if the content of 3-amino-2-naphthoic acid in a honey sample is between 17.59-620.01 mu g / kg, judging that the honey sample is apis cerana honey; and if the content of 3-amino-2-naphthoic acid in the honey sample is between 0.04-12.00 mu g / kg, judging that the honey sample is apis mellifera honey. The method provided by the invention is simple, convenient, accurate and convenient to operate and popularize, and has important practical significance for protecting legal rights and interests of honey consumers and maintaining healthy development of the honey consumption industry.

Description

Technical field [0001] The invention relates to the field of food detection, in particular to the application of 3-amino-2-naphthoic acid in the identification of bee honey and Italian bee honey. Background technique [0002] Honey is a natural sweet substance that bees collect from plant nectar, secretions or honeydew, combine with their own secretions, and fully brew them. Different honeybee species, nectar plants, origin, and even differences in harvesting and processing methods can lead to differences in endogenous active components in honey. Italian honeybees and Chinese honeybees are the two main bee species raised in my country. At present, there are about 9 million bee colonies in my country, among which the Italian honeybees are about 8 million colonies, and the Chinese honeybees are about 1 million colonies. Italian bee honey is collected and brewed from imported Italian bees from abroad. Italian bees are easy to raise, reproduce fast, produce high honey, and can colle...

AI Technical Summary

Problems solved by technology

Due to the differences in production methods and quality between Chinese bee honey and Italian bee honey, there is a significant difference in price between the two. Therefore, in order to pursue high profits, some lawbreakers use Italian bee honey to pretend to be or mix it with Chinese bee honey. Well, it harms the interests of consumers and regular honey producers, and restricts the healthy development of the Chinese bee industry

[0003] In recent years, the development of the identification technology of Chinese bee honey and Italian bee honey has attracted extensive attention from scientific researchers and related bee product companies. Relying on proteins as characteristic biomarkers for identification requires complex sample pretreatment processes such as protein extraction, concentration determination, protein electrophoretic separation, and band identification, as well as complex MALDI-TOF-TOF technology, which does not meet the requirements of efficient and rapid identification development trend

Chinese patent application 20180008004.2 reports a method for identifying Chinese honey and Italian honey based on beeswax alkane, but as a volatile component, its content will decrease significantly with the prolongation of storage time, which is not conducive to accurate identification

In recent years, small molecular endogenous substances have been widely used in the identification of food authenticity and traceability due to their stable presence in the matrix and the convenience of extraction and determination. However, there are no relevant reports on the identification of Chinese honey and Italian honey.

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