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42results about How to "Maintain healthy development" patented technology

Method for measuring content of apis mellifera MRJP1 protein in honey by using liquid chromatography-tandem mass spectrometry

The invention provides a method for measuring the content of apis mellifera MRJP1 protein (major royal jelly protein 1) in honey by using liquid chromatography-tandem mass spectrometry. The method comprises the steps of selecting characteristic peptides, preparing a standard curve, pre-treating a sample, performing liquid chromatography separation, and detecting and quantifying tandem mass spectrometry. The method for detecting the content of apis mellifera MRJP1 in honey provided by the invention has the advantages of strong specificity, high sensitivity, good accuracy and precision, and is suitable for accurate quantification of apis mellifera MRJP1 in honey. Since the content of MRJP1 in honey is relatively constant, the method can be used for evaluating the authenticity of honey basedon the content and assisting in identifying whether honey is adulterated or not. In addition, apis cerana honey does not contain apis mellifera MRJP1 and thus can be used for identifying whether apismellifera is mixed in the apis cerana honey. The method is of great significance for maintaining the healthy development of the honey consumption industry and the rights and interests of honey consumers.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI

Ecological system for comprehensively treating rural rain and sewage in hilly areas

The invention discloses an ecological system for comprehensively treating rural rainwater and sewage in hilly areas. The ecological system is composed of an improved three-grid septic tank, an ecological pond, a water collecting well, a flow discarding device, an infiltration well, a first valve and a second valve. Rural rainwater and domestic sewage in the hilly areas are comprehensively treatedin an ecological treatment mode. The domestic sewage treatment system comprises the improved three-grid septic tank and the ecological pond, wherein the ecological pond consists of a drop-type ecological water distribution channel and a main body. A rainwater runoff treatment system comprises the water collecting well, the discarding device and the infiltration well, rainwater runoff is filtered,infiltrated and accumulated through the infiltration well to be used for supplying underground water, and an overflow part is used for guiding water to activate the ecological pond or used for agricultural irrigation and direct drainage of rivers together with effluent of the ecological pond. The ecological system is novel in structure, rainwater and sewage are collected in an unpowered self-flowing mode, rural rainwater and sewage comprehensive treatment and water resource utilization are achieved, and the system has the advantages of being free of power output, low in construction cost, convenient to operate and maintain, outstanding in economic and environmental benefits and the like.
Owner:JIANGXI THERMAL POWER CONSTR CORP

A liquid chromatography tandem mass spectrometry method for the detection of royal jelly major protein 5 in Italian honey and Chinese honey

The invention provides a method for detecting major royal jelly protein 5 (MRJP5) of apis cerana honey and apis mellifera honey by liquid chromatography-tandem mass spectrometry. The method comprisesthe following steps: screening characteristic peptide fragments respectively belonging to apis cerana MRJP5 and apis mellifera MRJP5, establishing a liquid chromatography tandem mass spectrometry detection method, pretreating a honey sample, carrying out liquid chromatography separation, carrying out tandem mass spectrometry detection, carrying out data analysis and the like. The characteristic peptide fragments comprises an apis mellifera MRJP5 characteristic peptide fragment: YLDYDFGSDER, and an apis cerana MRJP5 characteristic peptide fragment: NLANSMNVIHEWK. The method for detecting MRJP5in honey has high accuracy, precision and sensitivity and strong stability, is suitable for identifying the apis mellifera honey and apis cerana honey, and is especially suitable for identifying adulteration of the apis cerana honey mixed with theapis mellifera honey. The method has important practical significance for protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI

Monoclonal antibody against Italian bee mrjp1 and colloidal gold detection card

The invention relates to the field of biotechnology and immunodetection, in particular to an anti-apis mellifera MRJP1 monoclonal antibody and a colloidal gold detection card. The invention provides ahybridoma cell strain 1F 61D 7 and a hybridoma cell strain 6F 124B 4 which stably secrete the anti-apis mellifera MRJP1 monoclonal antibody, the secreted antibody only recognizes apis mellifera MRJP1protein, does not recognize apis cerana MRJP1 protein, has the advantages of good specificity, high sensitivity and the like, and has no cross reaction with other family related proteins. The colloidal gold detection card and test strip of the apis mellifera MRJP1 are further developed from the antibody, the colloidal gold detection card and test strip have the advantages of high precision, smallbatch difference and the like, rapid detection of the apis mellifera MRJP1 in a bee product is achieved, and the colloidal gold detection card and test strip are particularly suitable for high-efficiency and large-batch detection of whether apis mellifera honey is mixed into Chinese honey (local honey) or not, and are particularly suitable for high-efficiency and large-batch detection of whetherapis mellifera honey is mixed into Chinese honey (local honey) or not
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI

Method for detecting apis mellifera and apis cerana glucose dehydrogenase in honey through liquid chromatography-tandem mass spectrometry

The invention relates to a method for detecting apis mellifera and apis cerana honey glucose dehydrogenase through liquid chromatography-tandem mass spectrometry. The method comprises the following steps: selecting characteristic peptide fragments respectively belonging to glucose dehydrogenase of apis mellifera honey and apis cerana honey, establishing a liquid chromatography-tandem mass spectrometry detection method, pretreating a sample, performing liquid chromatography separation, performing tandem mass spectrometry detection and performing data analysis, wherein the characteristic peptidefragments comprise an apis mellifera glucose dehydrogenase characteristic peptide fragment, namely ITLDNQSVQVR, and an apis cerana glucose dehydrogenase characteristic peptide fragment, namely FPYQPPFAWEILK. The method for detecting glucose dehydrogenase in honey has high accuracy, precision and sensitivity and strong stability and is suitable for distinguishing apis mellifera honey from apis cerana honey and is especially suitable for identifying apis mellifera honey doped in apis cerana honey. The method has important practical significance for protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI

A liquid chromatography tandem mass spectrometry method for detecting glucose dehydrogenase in the honey bees and bees

The invention relates to a method for detecting apis mellifera and apis cerana honey glucose dehydrogenase through liquid chromatography-tandem mass spectrometry. The method comprises the following steps: selecting characteristic peptide fragments respectively belonging to glucose dehydrogenase of apis mellifera honey and apis cerana honey, establishing a liquid chromatography-tandem mass spectrometry detection method, pretreating a sample, performing liquid chromatography separation, performing tandem mass spectrometry detection and performing data analysis, wherein the characteristic peptidefragments comprise an apis mellifera glucose dehydrogenase characteristic peptide fragment, namely ITLDNQSVQVR, and an apis cerana glucose dehydrogenase characteristic peptide fragment, namely FPYQPPFAWEILK. The method for detecting glucose dehydrogenase in honey has high accuracy, precision and sensitivity and strong stability and is suitable for distinguishing apis mellifera honey from apis cerana honey and is especially suitable for identifying apis mellifera honey doped in apis cerana honey. The method has important practical significance for protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI
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