42results about How to "Maintain healthy development" patented technology

The invention relates to a client server, an intelligent terminal, an online game system and a method, wherein the client server communicates with a client and a main server and comprises a local database, an adjustment module and a local logic module; the local database is used for storing information from the client; the adjustment module is used for judging whether a request from the client can be processed locally at the client serve; and the local logic module is used for processing the request from the client when the judgment module judges that the request from the client can be processed locally at the client serve, and is also used for synchronizing the information stored in the local database to the main server. The invention can be used to relieve the load of the main server of an online game and prevent plug-in and other cheating means.

The invention discloses a route abnormal detection method in domain based on multiview to solve the problem that can not find out hidden route abnormal or false abnormal report in prior art, which comprises: designing a route abnormal detection system to finish two stages work as: Internet mode construction by BGP route list information and other network topological knowledge to build Internet hierarchy relation mode and commercial interlinkage relation mode for ISP; abnormal detection and report for first general detection then special detection and treating abnormal route and generating report. This invention reinforces route monitoring system capacity obviously.

The invention provides a method for measuring the content of apis mellifera MRJP1 protein (major royal jelly protein 1) in honey by using liquid chromatography-tandem mass spectrometry. The method comprises the steps of selecting characteristic peptides, preparing a standard curve, pre-treating a sample, performing liquid chromatography separation, and detecting and quantifying tandem mass spectrometry. The method for detecting the content of apis mellifera MRJP1 in honey provided by the invention has the advantages of strong specificity, high sensitivity, good accuracy and precision, and is suitable for accurate quantification of apis mellifera MRJP1 in honey. Since the content of MRJP1 in honey is relatively constant, the method can be used for evaluating the authenticity of honey basedon the content and assisting in identifying whether honey is adulterated or not. In addition, apis cerana honey does not contain apis mellifera MRJP1 and thus can be used for identifying whether apismellifera is mixed in the apis cerana honey. The method is of great significance for maintaining the healthy development of the honey consumption industry and the rights and interests of honey consumers.

The invention provides a method for determining apis cerana Fabricius MRJP1 protein content (major royal jelly protein 1) in honey by utilizing liquid chromatography tandem mass spectrum. The method comprises the following steps: selecting characteristic peptide fragment, manufacturing a standard curve, pretreating the sample, performing liquid chromatographic separation and tandem mass spectrum detection quantitation and like steps. The detection method of apis cerana Fabricius MRJP1 content in honey has high accuracy, precision and sensitivity, and strong in stability, and suitable for the accurate quantitation of the apis cerana Fabricius MRJP1. The method further can be used for the truth evaluation of the honey, thereby assisting to identify whether the honey is adulterated, and is ofgreat significance for maintaining the healthy development of the honey consumption industry and the rights and interest of the honey consumer.

The invention provides a method for quantifying Apis mellifera honey MRJP2 through liquid chromatography-tandem mass spectrometry. Characteristic peptide fragments of Apis mellifera honey royal jellymajor protein 2 (MRJP2) are screened out, a quantitative method of liquid chromatography tandem mass spectrometry is developed, and the method comprises the steps of screening of the characteristic peptide fragments, making of a standard curve, performing sample pretreatment, performing liquid chromatography separation and tandem mass spectrometry detection, carrying out software quantification ofthe peptide fragments and the like. The method for quantifying the MRJP2 in the Apis mellifera honey, provided by the invention, has the advantages of higher accuracy, precision and sensitivity and strong stability, and is suitable for accurately quantifying the MRJP2 in the Apis mellifera honey. The method can be used for assisting authenticity evaluation of Apis mellifera honey. In addition, the quantitative method has important practical significance in protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.

The invention relates to the field of food detection, and particularly discloses an application of 3-amino-2-naphthoic acid in identification of apis cerana honey and apis mellifera honey. Meanwhile,the invention provides a method for identifying the apis cerana honey and the apis mellifera honey by taking the content of the 3-amino-2-naphthoic acid as an identification basis. The method comprises the steps of: if the content of 3-amino-2-naphthoic acid in a honey sample is between 17.59-620.01 mu g / kg, judging that the honey sample is apis cerana honey; and if the content of 3-amino-2-naphthoic acid in the honey sample is between 0.04-12.00 mu g / kg, judging that the honey sample is apis mellifera honey. The method provided by the invention is simple, convenient, accurate and convenient to operate and popularize, and has important practical significance for protecting legal rights and interests of honey consumers and maintaining healthy development of the honey consumption industry.

The invention provides a method for quantitatively detecting alpha-glucosidase in Italian honey through liquid chromatography-tandem mass spectrometry. The method comprises the steps of screening characteristic peptide fragments, making a standard curve, pretreating a sample, performing liquid chromatography separation, performing tandem mass spectrometry detection, quantifying the peptide fragments through software and the like. The characteristic peptide fragments comprise a characteristic peptide fragment 1: SISNNEQVK and a characteristic peptide fragment 2: IYTHDIPETYNVVR. The quantitativemethod for alpha-glucosidase in Italian honey provided by the invention has relatively high accuracy, precision and sensitivity and strong stability, is suitable for accurately quantifying alpha-glucosidase in Italian honey, and can be used for assisting authenticity evaluation of Italian honey. The quantitative method has important practical significance for protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.

The invention provides a method for quantifying Apis mellifera honey MRJP3 through liquid chromatography-tandem mass spectrometry. Aiming at the Apis mellifera honey, characteristic peptide fragmentsof royal jelly major protein 3 (MRJP3) in the Apis mellifera honey are screened out, a quantitative method of liquid chromatography tandem mass spectrometry is developed, and the method comprises thesteps of screening the characteristic peptide fragments, making a standard curve, carrying out sample pretreatment, carrying out liquid chromatography separation, carrying out tandem mass spectrometrydetection, carrying out software quantification on the peptide fragments and the like. The method for quantifying the MRJP3 in the Apis mellifera honey, provided by the invention, has the advantagesof higher accuracy, precision and sensitivity and strong stability, and is suitable for accurately quantifying the MRJP3 in the Apis mellifera honey. The method can be used for assisting authenticityevaluation of Apis mellifera honey. In addition, the quantitative method has important practical significance in protecting rights and interests of honey consumers and maintaining healthy developmentof the bee product industry.

The invention relates to the field of food detection, in particular to an application of high-content DSM (4-(2-hydroxy isopropyl)-1, 3-cyclohexadiene-1-carboxylic acid-isomaltose) as a linden honey characteristic marker. Meanwhile, the invention provides a basswood honey authenticity evaluation method, and the method comprises the steps: taking DSM as a characteristic compound; when the content of DSM in honey ranges from 250 mg / kg to 450 mg / kg, judging DSM as basswood honey; if not, determining that the honey is other honey or adulterated with linden honey. The method provided by the invention has the advantages of simplicity, high efficiency and the like, is convenient to operate and popularize, has certain guiding significance for formulating related industries or national standards ofhigh-quality linden honey, and also has important practical significance for protecting legitimate rights and interests of honey consumers and maintaining healthy development of the honey consumptionindustry.

The invention provides a method for quantifying Apis cerana cerana honey MRJP2 through liquid chromatography-tandem mass spectrometry. The characteristic peptide fragment of royal jelly major protein2 (MRJP2) is screened out for Apis cerana cerana honey, a quantitative method of liquid chromatography tandem mass spectrometry is developed, and the method comprises the steps of screening of the characteristic peptide fragment, making of a standard curve, performing sample pretreatment, performing liquid chromatography separation and tandem mass spectrometry detection, and carrying out softwarequantification of the peptide fragment and the like. The quantitative method for the Apis cerana cerana honey MRJP2 provided by the invention has the advantages of high accuracy, precision and sensitivity and strong stability, and is suitable for accurately quantifying the Apis cerana cerana honey MRJP2. The method can be used for assisting Apis cerana cerana honey authenticity evaluation. In addition, the quantitative method has important practical significance in protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.

The invention belongs to the field of food detection, and particularly relates to an authenticity evaluation method of robinia pseudoacacia honey and application of the method in adulteration identification. According to the authenticity evaluation method, robinin is used as an indicating substance, and when the content of robinin in honey is 0.8-2.0 mg / kg, robinia pseudoacacia honey can be determined; and when the content of the robinin in the honey is lower than 0.8 mg / kg, the honey is judged as other honey or adulterated robinia pseudoacacia honey. The method provided by the invention has the advantages of simplicity, high efficiency and the like, is convenient to operate and popularize, has certain guiding significance for the formulation of related industries or national standards ofhigh-quality robinia pseudoacacia honey, and also has important practical significance for protecting legitimate rights and interests of honey consumers and maintaining the healthy development of thehoney consumption industry.

The invention discloses an application method of a special fertilizer for Taxus chinensis and relates to the technical field of agricultural chemistry. According to the method, the special fertilizer is applied respectively in three phases according to plant growth situations of Taxus chinensis. An application method of Taxus chinensis of 2-10 years is as follows: as for a Taxus chinensis planting region of 38-40 plants / mu, 240-250 kilograms of the special fertilizer for Taxus chinensis is applied for each mu and is applied in three times. 100-105 kilograms of the special fertilizer is applied for the first time 3-5 days before the Waking of Insects yearly; 80-85 kilograms of the special fertilizer is applied for the second time 3-5 days before Slight Heat yearly; and the residual fertilizer is applied for the third time 3-5 days before Frost's Descent yearly. Plants are covered with root-broken weeds after each time of fertilization, and plants are covered for a week with drip line of each plant's crown as the center for each time of fertilization. The invention provides the application method of the special fertilizer for Taxus chinensis so as to solve problems such as low yield and poor growth of present Taxus chinensis.

The invention discloses an application method of a special fertilizer for Taxus chinensis and relates to the technical field of agricultural chemistry. According to the method, the special fertilizer is applied respectively in three phases according to plant growth situations of Taxus chinensis. An application method of Taxus chinensis of 2-10 years is as follows: as for a Taxus chinensis planting region of 38-40 plants / mu, 240-250 kilograms of the special fertilizer for Taxus chinensis is applied for each mu and is applied in three times. 100-105 kilograms of the special fertilizer is applied for the first time 3-5 days before the Waking of Insects yearly; 80-85 kilograms of the special fertilizer is applied for the second time 3-5 days before Slight Heat yearly; and the residual fertilizer is applied for the third time 3-5 days before Frost's Descent yearly. Plants are covered with root-broken weeds after each time of fertilization, and plants are covered for a week with drip line of each plant's crown as the center for each time of fertilization. The invention provides the application method of the special fertilizer for Taxus chinensis so as to solve problems such as low yield and poor growth of present Taxus chinensis.

The invention discloses an ecological system for comprehensively treating rural rainwater and sewage in hilly areas. The ecological system is composed of an improved three-grid septic tank, an ecological pond, a water collecting well, a flow discarding device, an infiltration well, a first valve and a second valve. Rural rainwater and domestic sewage in the hilly areas are comprehensively treatedin an ecological treatment mode. The domestic sewage treatment system comprises the improved three-grid septic tank and the ecological pond, wherein the ecological pond consists of a drop-type ecological water distribution channel and a main body. A rainwater runoff treatment system comprises the water collecting well, the discarding device and the infiltration well, rainwater runoff is filtered,infiltrated and accumulated through the infiltration well to be used for supplying underground water, and an overflow part is used for guiding water to activate the ecological pond or used for agricultural irrigation and direct drainage of rivers together with effluent of the ecological pond. The ecological system is novel in structure, rainwater and sewage are collected in an unpowered self-flowing mode, rural rainwater and sewage comprehensive treatment and water resource utilization are achieved, and the system has the advantages of being free of power output, low in construction cost, convenient to operate and maintain, outstanding in economic and environmental benefits and the like.

The invention relates to the field of food detection, particularly to application of high-content 2-isopropylmalic acid in pollen pini authenticity evaluation, and provides a pollen pini authenticityevaluation method, wherein 2-isopropylmalic acid is used as a characteristic compound, when the content of 2-isopropylmalic acid is 18-35 mg / kg, a sample can be judged as pollen pini, and otherwise, it is determined that the pollen is other pollen or adulterated pollen pini. The method provided by the invention is simple, efficient, high in stability and suitable for accurately determining the content of 2-isopropylmalic acid in pollen pini, is suitable for evaluating the authenticity and purity of the pollen pini, and has important practical significance for protecting legitimate rights and interests of consumers and maintaining healthy development of the pollen pini industry.

The invention relates to the field of food detection, in particular to the detection of high-content DSM (4-(2-hydroxyisopropyl)-1,3-cyclohexadiene-1-carboxylic acid-isomaltose) as a characteristic marker of lime tree honey application. Simultaneously, the present invention proposes a kind of authenticity evaluation method of linden tree honey, takes DSM as characteristic compound, when its content in honey is between 250-450 mg / kg, it can be judged as linden tree honey; No Otherwise, it is judged as other honey or adulterated linden honey. The method provided in the present invention has the advantages of simplicity, high efficiency, etc., is easy to operate and promote, and has certain guiding significance for the formulation of relevant industries or national standards for high-quality linden honey, and at the same time, it is useful for protecting the legitimate rights and interests of honey consumers and maintaining honey. The healthy development of the consumer industry has important practical significance.

The invention relates to the field of biotechnology and immunodetection, in particular to an anti-apis mellifera MRJP1 monoclonal antibody and a colloidal gold detection card. The invention provides ahybridoma cell strain 1F 61D 7 and a hybridoma cell strain 6F 124B 4 which stably secrete the anti-apis mellifera MRJP1 monoclonal antibody, the secreted antibody only recognizes apis mellifera MRJP1protein, does not recognize apis cerana MRJP1 protein, has the advantages of good specificity, high sensitivity and the like, and has no cross reaction with other family related proteins. The colloidal gold detection card and test strip of the apis mellifera MRJP1 are further developed from the antibody, the colloidal gold detection card and test strip have the advantages of high precision, smallbatch difference and the like, rapid detection of the apis mellifera MRJP1 in a bee product is achieved, and the colloidal gold detection card and test strip are particularly suitable for high-efficiency and large-batch detection of whether apis mellifera honey is mixed into Chinese honey (local honey) or not, and are particularly suitable for high-efficiency and large-batch detection of whetherapis mellifera honey is mixed into Chinese honey (local honey) or not

The invention relates to a client server, an intelligent terminal, an online game system and a method, wherein the client server communicates with a client and a main server and comprises a local database, an adjustment module and a local logic module; the local database is used for storing information from the client; the adjustment module is used for judging whether a request from the client can be processed locally at the client serve; and the local logic module is used for processing the request from the client when the judgment module judges that the request from the client can be processed locally at the client serve, and is also used for synchronizing the information stored in the local database to the main server. The invention can be used to relieve the load of the main server ofan online game and prevent plug-in and other cheating means.

The invention provides a method for quantifying Apis cerana cerana honey MRJP2 through liquid chromatography-tandem mass spectrometry. The characteristic peptide fragment of royal jelly major protein2 (MRJP2) is screened out for Apis cerana cerana honey, a quantitative method of liquid chromatography tandem mass spectrometry is developed, and the method comprises the steps of screening of the characteristic peptide fragment, making of a standard curve, performing sample pretreatment, performing liquid chromatography separation and tandem mass spectrometry detection, and carrying out softwarequantification of the peptide fragment and the like. The quantitative method for the Apis cerana cerana honey MRJP2 provided by the invention has the advantages of high accuracy, precision and sensitivity and strong stability, and is suitable for accurately quantifying the Apis cerana cerana honey MRJP2. The method can be used for assisting Apis cerana cerana honey authenticity evaluation. In addition, the quantitative method has important practical significance in protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.

The invention relates to the technical field of food detection, in particular to a method for identifying the maturity of acacia honey. The identification method of acacia honey maturity provided by the present invention is to use turanose as a characteristic marker to detect acacia honey samples, if the content of acacia honey ≥ 0.5% in acacia honey samples, then judge that acacia honey samples are mature acacia honey, if If the content of turanose in the acacia honey sample is greater than or equal to 0.3% and less than 0.5%, it is judged that the acacia honey sample is semi-mature acacia honey, and if the content of turanose in the acacia honey sample is <0.3%, it is judged that the acacia honey sample is immature acacia honey honey. The method has the characteristics of simple operation, high efficiency, low cost, etc., reliable and stable detection results, high degree of judgment, can be used for quality evaluation and grading of acacia honey, and is convenient for popularization and application.

The invention discloses a method for adjusting seawater net cage water body ecology through poly-culture of wild China Monacanthus chinensis. The method comprises steps that wild China Monacanthus chinensis and seawater main bred fish types are poly-cultured; casting density of the wild China Monacanthus chinensis is 5 to 10 fishes / m<3>; and coverage of v on the poly-culturing net cage during the cast is 10 to 20%. Wild China Monacanthus chinensis and seawater main bred fish types are poly-cultured and the two types have no food competition relationship; the wild China Monacanthus chinensis has no effect on normal growth of the seawater bred fish types and can also absorb alga attached to the net cloth during the cultivation of the net cage and residual baits or faeces of each day; net cage water quality decrease due to the alga, the residual baits and the faeces can be reduced, so healthy development of the seawater net cage ecological system can be stably and effectively maintained; and culture production can be normally conducted.

The invention relates to the field of food detection, in particular to an application of HQR (4-hydroxyquinoline-beta-rutinoside) as a characteristic marker of schefflera octophylla honey. Meanwhile,the invention provides an authenticity evaluation method of the schefflera octophylla honey, HQR is used as a characteristic compound, and when the content of the HQR in the honey is 8-10 mg / kg, schefflera octophylla honey can be decided, or otherwise, the honey is decided as other honey or adulterated schefflera octophylla honey. The method for detecting the schefflera octophylla honey has the advantages of simplicity, high efficiency and the like, is convenient to operate and popularize, has a certain guiding significance for formulating relevant industry standards of high-quality scheffleraoctophylla honey, and has an important practical significance for protecting legitimate rights and interests of honey consumers and maintaining healthy development of the honey consumption industry.

The invention relates to a method for detecting apis mellifera and apis cerana honey glucose dehydrogenase through liquid chromatography-tandem mass spectrometry. The method comprises the following steps: selecting characteristic peptide fragments respectively belonging to glucose dehydrogenase of apis mellifera honey and apis cerana honey, establishing a liquid chromatography-tandem mass spectrometry detection method, pretreating a sample, performing liquid chromatography separation, performing tandem mass spectrometry detection and performing data analysis, wherein the characteristic peptidefragments comprise an apis mellifera glucose dehydrogenase characteristic peptide fragment, namely ITLDNQSVQVR, and an apis cerana glucose dehydrogenase characteristic peptide fragment, namely FPYQPPFAWEILK. The method for detecting glucose dehydrogenase in honey has high accuracy, precision and sensitivity and strong stability and is suitable for distinguishing apis mellifera honey from apis cerana honey and is especially suitable for identifying apis mellifera honey doped in apis cerana honey. The method has important practical significance for protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.

The invention relates to a method for detecting apis mellifera and apis cerana honey glucose dehydrogenase through liquid chromatography-tandem mass spectrometry. The method comprises the following steps: selecting characteristic peptide fragments respectively belonging to glucose dehydrogenase of apis mellifera honey and apis cerana honey, establishing a liquid chromatography-tandem mass spectrometry detection method, pretreating a sample, performing liquid chromatography separation, performing tandem mass spectrometry detection and performing data analysis, wherein the characteristic peptidefragments comprise an apis mellifera glucose dehydrogenase characteristic peptide fragment, namely ITLDNQSVQVR, and an apis cerana glucose dehydrogenase characteristic peptide fragment, namely FPYQPPFAWEILK. The method for detecting glucose dehydrogenase in honey has high accuracy, precision and sensitivity and strong stability and is suitable for distinguishing apis mellifera honey from apis cerana honey and is especially suitable for identifying apis mellifera honey doped in apis cerana honey. The method has important practical significance for protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.