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Application of high content dsm as a characteristic marker of linden honey

A linden tree honey, high-content technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as missing

Inactive Publication Date: 2020-08-11
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the industry has not formulated any standards or industry standards related to linden honey, and the lack of relevant standards and identification methods provides the possibility of adulteration in the current linden honey market.

Method used

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  • Application of high content dsm as a characteristic marker of linden honey
  • Application of high content dsm as a characteristic marker of linden honey
  • Application of high content dsm as a characteristic marker of linden honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] 1. Sample source

[0065] A total of 80 common real honey samples were purchased from the market or beekeepers to test the content of DSM in various honeys, including: acacia, jujube flower, rapeseed, lychee, duck foot wood, longan, pomegranate, snow lotus , caragana, wild bazi, old melon head, medlar, codonopsis, eucalyptus, manuka, wild osmanthus, tallow, sunflower honey four samples each, and 8 linden samples.

[0066] 2. Solution preparation

[0067] Extraction solution: pipette 20 mL of methanol, and dilute to 100 mL with ultrapure water. Store at 4°C.

[0068] DSM standard solution: weigh 10 mg of DSM standard, and dilute to 10 mL with methanol. Store at 4 ℃, valid for 2 months.

[0069] 3. Q Exactive plus test conditions are as follows:

[0070] (1) Sample processing:

[0071] Weigh 0.8 g of honey and add 7.2 mL of extraction solution, vortex at room temperature until completely dissolved, and in 20194 g , centrifuged at 4°C for 20 min. The supernatant wa...

Embodiment 2

[0083] 1. Sample source

[0084] A total of 100 honey samples of various grades or brands labeled as linden honey were purchased from the market.

[0085] 2. Experimental procedure

[0086] (1) Solution preparation

[0087] Extraction solution: pipette 20 mL of methanol, and dilute to 100 mL with ultrapure water. Store at 4°C.

[0088] DSM standard solution: weigh 10 mg of DSM standard, and dilute to 10 mL with methanol. Store at 4 ℃, valid for 2 months.

[0089] (2) Sample processing:

[0090] Weigh 0.8 g of honey and add 7.2 mL of extraction solution, vortex at room temperature until completely dissolved, and in 20194 g , centrifuged at 4°C for 20 min. The supernatant was collected and passed through a 0.22 µm filter membrane, 990 µL of the extract was added to the sample bottle, and 10 µL of the liquid after passing through the membrane was transferred to the sample bottle, vortexed and mixed, waiting for analysis on the machine.

[0091] (3) Quality control samples...

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Abstract

The invention relates to the field of food detection, in particular to the detection of high-content DSM (4-(2-hydroxyisopropyl)-1,3-cyclohexadiene-1-carboxylic acid-isomaltose) as a characteristic marker of lime tree honey application. Simultaneously, the present invention proposes a kind of authenticity evaluation method of linden tree honey, takes DSM as characteristic compound, when its content in honey is between 250-450 mg / kg, it can be judged as linden tree honey; No Otherwise, it is judged as other honey or adulterated linden honey. The method provided in the present invention has the advantages of simplicity, high efficiency, etc., is easy to operate and promote, and has certain guiding significance for the formulation of relevant industries or national standards for high-quality linden honey, and at the same time, it is useful for protecting the legitimate rights and interests of honey consumers and maintaining honey. The healthy development of the consumer industry has important practical significance.

Description

technical field [0001] The invention relates to the field of food detection, in particular to the application of high-content DSM as a characteristic marker of linden tree honey. Background technique [0002] Honey is a natural sweet substance that is fully brewed by bees from the nectar, secretion or honeydew of plants collected by bees, combined with their own secretions. Due to the rich nutritional value of honey, it is favored by consumers, and its sales volume is also on the rise year by year. my country has a vast territory, complex terrain, diverse climates, rich resources, and rich and colorful nectar plants. Rapeseed, acacia, jujube, Vitex, linden, milk vetch, lychee, loquat, etc. are the main nectar plants in my country and the main source of bulk honey production in China, and its unifloral nectar is the main form of honey in the market in my country. Tilia bran and Tilia are the main species of linden, and they are mainly distributed in the Changbai Mountains, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/047
Inventor 杨术鹏李熠谭海广周金慧张金震金钥杨宇辉黄京平袁媛
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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