Application of hqr as a characteristic marker of Duckfoot honey

The technology of duck's feet honey and duck's feet is applied in the application field of the characteristic markers of duck's feet honey, and can solve the problems of high selling price, high production cost, limited output of duck's feet honey, etc.

Inactive Publication Date: 2020-10-23
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limited output and high production cost of nectar, the price of nectar is significantly higher than that of other common single-floral honeys

Method used

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  • Application of hqr as a characteristic marker of Duckfoot honey
  • Application of hqr as a characteristic marker of Duckfoot honey
  • Application of hqr as a characteristic marker of Duckfoot honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] In this example, firstly, through the positive and negative modes of liquid chromatography tandem high-resolution mass spectrometry, a large amount of nectar and other common single-floral nectars were scanned and analyzed, and it was found that nectar were all in the positive mode (such as figure 1 shown) in the case of the presence of high levels of substance peaks m / z 454.17077 (eg figure 2 shown), significantly different from other common unifloral nectars, the main fragment of this material peak contains m / z 146.06004 (eg image 3 shown). Accurate mass based on high-resolution mass spectrometry m / z 454.17077, obtained the elemental composition of the substance as C 21 h 28 NO 10 + , with the main fragment m / z Compared with 146.06004, C is reduced in the elemental composition 12 h 20 o 9 ; Due to the possible dehydration reaction in the fragmentation process of the mass spectrum, it is speculated that the reduced substance may be C 12 h 22 o 10 . ...

Embodiment 2

[0066] 1. Sample source

[0067] A total of 90 common real honey samples were purchased from the market or beekeepers to test the content of HQR in various honeys, including: acacia, jujube flower, rapeseed, litchi, longan, Vitex, eucalyptus, snow lotus, sunflower , Chinese tallow tree, pomegranate, wild osmanthus, wild bazi, caragana, manuka, old melon head, wolfberry, Codonopsis pilosula, gallnut, citrus honey, and 10 duck foot wood samples.

[0068] 2. Solution preparation

[0069] Extraction solution: pipette 20 mL of methanol, and dilute to 100 mL with ultrapure water. Store at 4°C.

[0070] HQR standard solution: Weigh 10 mg of HQR standard and dilute to 10 mL with methanol. Store at 4 ℃, valid for 2 months.

[0071] 3. Q Exactive plus test conditions are as follows:

[0072] (1) Sample processing:

[0073] Weigh 0.8 g of honey and add 7.2 mL of extraction solution, vortex at room temperature until completely dissolved, and in 20194 g , centrifuged at 4°C for 20 min...

Embodiment 3

[0085] 1. Sample source

[0086] A total of 50 samples of various grades or brands of honey labeled as Duckfoot Honey were purchased from the market.

[0087] 2. Experimental procedure

[0088] (1) Solution preparation

[0089] Extraction solution: pipette 20 mL of methanol, and dilute to 100 mL with ultrapure water. Store at 4°C.

[0090] HQR standard solution: Weigh 10 mg of HQR standard and dilute to 10 mL with methanol. Store at 4 ℃, valid for 2 months.

[0091] (2) Sample processing:

[0092] Weigh 0.8 g of honey and add 7.2 mL of extraction solution, vortex at room temperature until completely dissolved, and in 20194 g , centrifuged at 4°C for 20 min. The supernatant was collected and passed through a 0.22 µm filter membrane, 950 µL of the extract was added to the sample bottle, and 50 µL of the liquid after passing through the membrane was transferred to the sample bottle, vortexed and mixed, and waited for analysis on the machine.

[0093] (3) Quality control s...

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Abstract

The invention relates to the field of food detection, in particular to an application of HQR (4-hydroxyquinoline-beta-rutinoside) as a characteristic marker of schefflera octophylla honey. Meanwhile,the invention provides an authenticity evaluation method of the schefflera octophylla honey, HQR is used as a characteristic compound, and when the content of the HQR in the honey is 8-10 mg / kg, schefflera octophylla honey can be decided, or otherwise, the honey is decided as other honey or adulterated schefflera octophylla honey. The method for detecting the schefflera octophylla honey has the advantages of simplicity, high efficiency and the like, is convenient to operate and popularize, has a certain guiding significance for formulating relevant industry standards of high-quality scheffleraoctophylla honey, and has an important practical significance for protecting legitimate rights and interests of honey consumers and maintaining healthy development of the honey consumption industry.

Description

technical field [0001] The invention relates to the field of food detection, and in particular to the application of HQR as a characteristic marker of japonica honey. Background technique [0002] Honey is a natural sweet substance that bees collect nectar from nectar source plants and mix with their own secretions, and then fully brewed. Because honey is rich in nutrients and has certain effects such as beauty, intestines, and health care, it is deeply loved by people. In the prior art, there are many types of nectar source plants, including linden, sunflower, acacia, jujube flower, vitex, chestnut, cotton, melon head, wolfberry, caragana, alfalfa, astragalus, codonopsis, vetch, pomegranate, rubber, Wild dam, rapeseed, citrus, tallow, pipa, litchi, longan, duck foot wood, etc. The same species of nectar source plants have the same flowering period and are geographically distributed in a concentrated manner, which forms the basis for beekeepers to produce single flower nec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/32G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/32G01N30/34G01N30/72G01N2030/324
Inventor 杨术鹏李熠谭海广周金慧张金震杨宇晖
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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