2-decenedioic acid serving as indicative substance for honey authenticity evaluation and application of 2-decenedioic acid in honey adulteration identification

A decenedioic acid, authenticity technology, applied in the field of application in the identification of honey adulteration, can solve the problem of shortening the life cycle

Inactive Publication Date: 2020-10-23
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, with the improvement of the fructose syrup production process, its purity continues to increase. The currently developed honey adulteration identification method often lags behind the improvement of the fructose syrup process. The cycle is getting shorter

Method used

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  • 2-decenedioic acid serving as indicative substance for honey authenticity evaluation and application of 2-decenedioic acid in honey adulteration identification
  • 2-decenedioic acid serving as indicative substance for honey authenticity evaluation and application of 2-decenedioic acid in honey adulteration identification
  • 2-decenedioic acid serving as indicative substance for honey authenticity evaluation and application of 2-decenedioic acid in honey adulteration identification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Excavation and structure identification of endogenous substance 2-decenedioic acid in honey

[0058] In this embodiment, first, a large amount of honey and other common honeys are sampled and analyzed by the positive and negative modes of liquid chromatography tandem high-resolution mass spectrometry. The experimental results show that there is a compound with a higher content in the total ion chromatogram of honey in negative mode, such as figure 2 As shown, A is the total ion chromatogram of the honey sample, B is the precise extraction ion chromatogram of 2-decenedioic acid in the honey sample (mass extraction window is 5 ppm), and its chromatographic retention time is 9.38 min, The exact mass is m / z 199.09702.

[0059] Accurate mass based on high-resolution mass spectrometry m / z 199.09758 (R as image 3 Middle A figure), the present embodiment obtains the elemental composition of this material to be C 10 h 15 o 4 - . In the MS / MS spectrum of th...

Embodiment 2

[0061] Example 2 Contents of 2-decenedioic acid in different honey samples

[0062] 1. Sample source

[0063] Across the country, more than 500 common real honey samples were directly collected from beekeepers in different seasons and years to investigate the content of 2-decenedioic acid in various honeys. Species of bees are: acacia, jujube flower, rape, lychee, longan, vitex, eucalyptus, snow lotus, sunflower, tallow, pomegranate, wild osmanthus, wild dam, caragana, manuka, melon head, Goji berry, duck foot wood, Codonopsis pilosula, Galla, citrus honey samples, the number of single-flower honey samples of each variety is no less than 20.

[0064] 2. Solution preparation

[0065] Extraction solution: Pipette 20 mL of methanol, dilute to 100 mL with ultrapure water, and store at 4 °C.

[0066] 2-Decenedioic acid standard solution: Weigh 2 mg of decenedioic acid standard product, dilute to 2 mL with methanol, and store in a freezer at -20 ℃, with a validity period of 6 month...

Embodiment 3

[0081] Example 3 Application of 2-decenedioic acid as an indicator substance in the identification of honey adulteration

[0082] 1. Sample source

[0083] A total of 20 honey samples of various grades or brands labeled as honey were purchased from the market.

[0084] 2. Experimental steps

[0085] (1) Solution preparation

[0086] Extraction solution: pipette 20 mL of methanol, and dilute to 100 mL with ultrapure water. Store at 4°C.

[0087] 2-Decenedioic acid standard solution: Weigh 2 mg of decenedioic acid standard product, dilute to 2 mL with methanol, store at -20 ℃, valid for 6 months.

[0088] (2) Sample processing

[0089] Weigh 0.8 g of honey and add 7.2 mL of extraction solution, vortex at room temperature until completely dissolved, and in 20194 g , centrifuged at 4°C for 20 min. The supernatant was collected and passed through a 0.22 µm filter membrane, and then 100 µL of the extract: 900 µL of methanol and water was diluted once. The diluent was transfe...

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Abstract

The invention relates to the field of food detection, in particular to 2-decenedioic acid serving as an indicative substance for honey authenticity evaluation and application of the 2-decenedioic acidin honey adulteration identification. According to an authenticity evaluation method of honey, 2-decendioic acid is used as an indicative substance, and if the content of the 2-decendioic acid in a honey sample is 4.0-8.0mg/kg, the honey sample is judged to be real honey; and if the content of 2-decendioic acid in the honey sample is lower than 4.0mg/kg, the honey sample is judged to be adulterated honey. The method provided by the invention has the advantages of simplicity, high efficiency and the like, is convenient to operate and popularize, has certain guiding significance for formulatingrelated industry or national standards of honey authenticity evaluation, and also has important practical significance for protecting legitimate rights and interests of honey consumers and maintaining healthy development of the honey consumption industry.

Description

technical field [0001] The invention relates to the field of food detection, in particular to 2-decenedioic acid as an indicator substance for authenticity evaluation of honey and its application in identification of honey adulteration. Background technique [0002] Honey is a natural sweet food that bees collect nectar and honeydew from nectar source plants and their own secretions, fully brewed and stored in the hive. Because honey is rich in beneficial substances such as amino acids, phenolic acids, flavonoids, and vitamins, and has good effects such as beautifying the skin, moistening the intestines, and nourishing, it is deeply loved by the masses, but there are certain adulterations in the honey sold on the market. Behavior. Fructose, glucose and water are the main components of honey, and these three account for more than 95% of the total mass of honey. Therefore, honey is a commodity that is very easy to adulterate. According to the EU Food and Drug Administration ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634
Inventor 杨术鹏李熠张玉豪周金慧杨宇晖
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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