Application of high-content 2-isopropylmalic acid in pollen pini authenticity evaluation

An isopropyl and authenticity technology, which is applied in the application field of high-content 2-isopropylmalic acid in the authenticity evaluation of pine pollen, can solve the problem of high price, hindering the healthy and orderly development of the pine pollen industry, and harming consumption. Issues such as rights and interests of patients, to achieve high specificity and sensitivity

Inactive Publication Date: 2020-09-25
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the output of pine pollen is limited, the market gap is relatively large, and its price is high, usually several times higher than other pollen, such as rape pollen, camellia pollen, etc.
In order to pursue high profits, some unscrupulous merchants often mix pine pollen with starch or other varieties of pollen to carry out adulteration and sales, which not only damages the rights and interests of consumers, but also hinders the healthy and orderly development of the pine pollen industry
At present, my country has not formulated the evaluation method for the authenticity and purity of pine pollen, and how to evaluate the authenticity and purity of pine pollen has become an urgent problem recognized by the majority of pine pollen practitioners and consumers.

Method used

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  • Application of high-content 2-isopropylmalic acid in pollen pini authenticity evaluation
  • Application of high-content 2-isopropylmalic acid in pollen pini authenticity evaluation
  • Application of high-content 2-isopropylmalic acid in pollen pini authenticity evaluation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1. Sample source

[0058] A total of 50 common real pine pollen samples were purchased from the market or pine tree growers to detect the content of 2-isopropylmalic acid in various pine pollen, which originated from the following pine pollen from different origins: Jilin, Liaoning , Jiangsu, Shandong, Yunnan, Zhejiang, Fujian, including real pine pollen samples (10 parts) and several other common pollen, including: rapeseed pollen (10 parts), Codonopsis pollen (5 parts), corn pollen (7 parts) , lotus pollen (8 parts), mountain pollen (5 parts), rose pollen (5 parts).

[0059] 2. Solution preparation

[0060] 2-isopropylmalic acid standard solution: Weigh 10 mg of 2-isopropylmalic acid standard solution and dilute to 10 mL with methanol. Store at 4°C, valid for 2 months.

[0061] 3. The detection conditions of Q Exactive plus are as follows:

[0062] (1) Sample processing:

[0063] Weigh 0.5 g pine pollen sample: 5 mL methanol to mix it, and after fully dissolving ...

Embodiment 2

[0084] 1. Sample source

[0085] A total of 40 samples of various grades or brands of pine pollen labeled as pine pollen were purchased from the market.

[0086] 2. Experimental procedure

[0087] (1) Solution preparation

[0088] 2-isopropylmalic acid standard solution: Weigh 10 mg of 2-isopropylmalic acid standard solution and dilute to 10 mL with methanol. Store at 4°C, valid for 2 months.

[0089] (2) Sample processing:

[0090] Weigh 0.5 g of pine pollen sample and add it to 5 mL of methanol for mixing, and fully dissolve the pine pollen, 20194 g , centrifuged at 4 ℃ for 20 min, then took 100 µL of the extract, added to 900 µL of pure water for dilution, and then took 100 µL of the diluted solution, added it to 900 µL of pure water to dilute again. Transfer to a sample vial and vortex to mix well, waiting for analysis on the machine.

[0091] (2) Quality control samples:

[0092] Weigh 0.5 g of blank pollen (without 2-isopropylmalic acid) and add 5 mL of methanol, ...

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Abstract

The invention relates to the field of food detection, particularly to application of high-content 2-isopropylmalic acid in pollen pini authenticity evaluation, and provides a pollen pini authenticityevaluation method, wherein 2-isopropylmalic acid is used as a characteristic compound, when the content of 2-isopropylmalic acid is 18-35 mg / kg, a sample can be judged as pollen pini, and otherwise, it is determined that the pollen is other pollen or adulterated pollen pini. The method provided by the invention is simple, efficient, high in stability and suitable for accurately determining the content of 2-isopropylmalic acid in pollen pini, is suitable for evaluating the authenticity and purity of the pollen pini, and has important practical significance for protecting legitimate rights and interests of consumers and maintaining healthy development of the pollen pini industry.

Description

technical field [0001] The invention relates to the field of food testing, in particular to the application of high-content 2-isopropylmalic acid in authenticity evaluation of pine pollen. Background technique [0002] Pine pollen is the pollen produced by plants such as Pinus massoniana and Pinus tabulaeformis. It is a natural food and health product obtained through picking, drying, and impurity removal. It is widely used in food health care, skin care and beauty, and trauma treatment. Specifically, pine pollen is rich in various proteins, carbohydrates, minerals, nucleotides, flavonoids, monosaccharides, polysaccharides, etc., which can fully supplement and balance the nutrition needed by the human body, so it is popular among people ( Especially for the elderly), it has a good application in food or health care products. At the same time, because pine pollen has certain effects such as skin care and hemostasis, it is also widely used in women's beauty, baby skin care an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/7266G01N30/8679
Inventor 杨术鹏李熠蔡冬梅周金慧杨宇晖张金震
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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