The invention relates to the field of food detection, particularly to application of high-content 2-isopropylmalic acid in pollen pini authenticity evaluation, and provides a pollen pini authenticityevaluation method, wherein 2-isopropylmalic acid is used as a characteristic compound, when the content of 2-isopropylmalic acid is 18-35 mg / kg, a sample can be judged as pollen pini, and otherwise, it is determined that the pollen is other pollen or adulterated pollen pini. The method provided by the invention is simple, efficient, high in stability and suitable for accurately determining the content of 2-isopropylmalic acid in pollen pini, is suitable for evaluating the authenticity and purity of the pollen pini, and has important practical significance for protecting legitimate rights and interests of consumers and maintaining healthy development of the pollen pini industry.