30 results about "Isopropylmalic acid" patented technology

Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase.

A novel modified polypeptide having an isopropylmalate synthase activity, a polynucleotide encoding the same, a microorganism including the polypeptide, and a method of producing L-leucine by culturing the microorganism.

The invention provides 3-isopropyl malic dehydrogenase C which is extracted from cordyceps sinensis hirsutella sinensis and joins in biological catalysis on 3-isopropyl malic acid to prepare 4-methyl-2-oxo valeric acid, an encoding gene and application of 3-isopropyl malic dehydrogenase C. The amino acid sequence of 3-isopropyl malic dehydrogenase C is as shown in SEQ ID No.1, and the encoding gene is as shown in SEQ ID No.1; the clone DNA (Deoxyribose Nucleic Acid) of the nucleotide sequence can be transferred into engineering bacteria by using methods of transduction, conversion and conjugal transfer, by adjusting the expression of the gene of 3-isopropyl malic dehydrogenase C, the host 3-isopropyl malic dehydrogenase C is high in expression property, an effective method is provided to expand the biological application of 3-isopropyl malic dehydrogenase C, and great application prospects are achieved.

The present invention relates to a mutant, non-naturally occurring or transgenic plant cell comprising: (i) at least one polynucleotide comprising, consisting or consisting essentially of a sequence encoding an isopropylmalate synthase and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:10 or SEQ ID NO: 12 or SEQ ID NO:14; or (ii) a polypeptide encoded by said polynucleotide(s); or (iii) a polypeptide having at least 60% sequence identity to SEQ ID NO:2 or SEQ ID NO:11 or SEQ ID NO:13 or SEQ ID NO:15; or (iv) a construct, vector or expression vector comprising said polynucleotide sequence(s), optionally wherein said construct, vector or expression vector additionally comprises a promoter comprising, consisting or consisting essentially of the sequence set forth in SEQ ID NO:8 or a variant thereof with at least about 60% identity thereto or a trichome promoter.

The invention relates to a genetic engineering bacterium for producing L-leucine, and application of the genetic engineering bacterium, and belongs to the field of metabolic engineering. The genetic engineering bacterium is obtained by an isopropylmalate synthetase encoding gene leuA[M] capable of relieving L-leucine feedback inhibition through overexpression in a host cell, an acetolactate synthase encoding gene ilvBN[M] capable of relieving L-leucine feedback inhibition, a 3-isopropylmalate dehydrogenase encoding gene leuB and a 3-isopropylmalate dehydratase encoding gene leuCD. Acetohydroxyacid synthase encoded by the leuA[M] relieves the feedback inhibition function of the L-leucine for the acetohydroxyacid synthase, in addition, the activity of the acetohydroxyacid synthase is not obviously lowered than wild leuA encoded isopropylmalate synthetase. The L-leucine genetic engineering bacterium does not have nutritional deficiencies, is quick in growth, and has a short fermentation period, a high yield and a high conversion rate. After fermentation is conducted for 40-44h, the concentration of the L-leucine in fermentation liquor achieves 60.5-69.6g / L.