Processes to prepare elongated 2-ketoacids and C5-C10 compounds therefrom via genetic modifications to microbial metabolic pathways

A C6-C10, gene modification technology, applied in the field of microorganisms of isopropylmalate synthase, can solve the problem that microorganisms cannot produce

Active Publication Date: 2019-05-21
DOW GLOBAL TECH LLC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, microorganisms are often unable to produce many petrochemical-based products at economically viable rates or yields

Method used

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  • Processes to prepare elongated 2-ketoacids and C5-C10 compounds therefrom via genetic modifications to microbial metabolic pathways
  • Processes to prepare elongated 2-ketoacids and C5-C10 compounds therefrom via genetic modifications to microbial metabolic pathways
  • Processes to prepare elongated 2-ketoacids and C5-C10 compounds therefrom via genetic modifications to microbial metabolic pathways

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[0070] Preparation of a genetically modified isopropylmalate synthase with enhanced catalytic activity towards 2-ketohexanoate and 2-ketooctanoate.

[0071] During the biosynthesis of 2-ketononanoic acid via the recursive activity of the "+1" pathway of the leucine biosynthetic pathway, isopropylmalate synthase traps and condenses acetyl-CoA and 2-ketoacids of various lengths. For efficient biosynthesis of 2-ketononanoic acid, isopropylmalate synthase is expected to efficiently capture 2-ketobutyrate, 2-ketovalerate, 2-ketohexanoate, 2-ketoheptanoate and 2-ketoheptanoate with acetyl-CoA. and / or 2-ketooctanoic acid, resulting in the corresponding 2-alkylmalic acid product ( figure 1 Intermediate II) in. The native isopropylmalate synthase of E. coli and other microorganisms is less efficient at capturing longer unnatural 2-ketoacid substrates. To increase the activity of native isopropylmalate synthase to capture longer 2-keto acids for catalysis, the active site of isopropy...

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Abstract

Genetically modified isopropylmalate synthases, processes for preparing a C7-C11 2-ketoacids utilizing genetically modified isopropylmalate synthases, and microbial organisms including genetically modified isopropylmalate synthases are described. The genetically modified isopropylmalate synthases, processes for preparing a C7-C11 2-ketoacids, and microbial organisms including genetically modifiedisopropylmalate synthases can be particularly useful for producing corresponding Cn_1 aldehydes, alcohols, carboxylic acids, and Cn_2 alkanes both in vivo and in vitro.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Application Serial No. 62 / 402,586, filed September 30, 2016, and U.S. Provisional Application Serial No. 62 / 402,569, filed September 30, 2016, the entire contents of both applications Incorporated herein by reference. [0003] sequence listing [0004] This application incorporates by reference the 37,000 byte ASCII text file "79045-WO-PCT_SequenceListing.txt" created August 23, 2016 and filed September 30, 2016 in U.S. Provisional Application Serial No. 62 / 402,586 in the material. technical field [0005] The present disclosure relates to the use of biological enzymes to produce C 7 -C 11 2-Keto acids and products prepared therefrom. More specifically, the present disclosure relates to genetically modified isopropylmalate synthases, the use of such genetically modified isopropylmalate synthases to convert 2-ketoacid substrates to C 7 -C 11 2-keto acid, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/04C12N9/10C12P7/24C12P7/40
CPCC12Y402/01033C12N9/88C12P7/04C12P7/24C12P7/40C12P7/44C12N9/0006C12Y101/01085C12N15/52C12Y203/03013C12N9/1025Y02E50/10C12N9/0008C12P5/02C12Y102/01048
Inventor P·桑格哈尼S·德拉普兰
Owner DOW GLOBAL TECH LLC
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