Method for evaluating authenticity of robinia pseudoacacia honey and application of method in adulteration identification

An evaluation method, the technology of acacia honey, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the unreported evaluation methods of the authenticity and purity of acacia honey, etc., and achieve the effect of easy operation and promotion

Active Publication Date: 2021-01-29
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acacia honey is the main commercial honey in my country. At present, there is no report on the evaluation method of the authenticity and purity of acacia honey.

Method used

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  • Method for evaluating authenticity of robinia pseudoacacia honey and application of method in adulteration identification
  • Method for evaluating authenticity of robinia pseudoacacia honey and application of method in adulteration identification
  • Method for evaluating authenticity of robinia pseudoacacia honey and application of method in adulteration identification

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Experimental program
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Effect test

Embodiment 1

[0057] In this embodiment, first, a large amount of acacia honey and other common honeys were sampled and analyzed by the positive and negative modes of liquid chromatography tandem high-resolution mass spectrometry. Experimental result shows, in the total ion current chromatogram that retention time is 6.80min, there is a compound with higher content in acacia honey, and this compound does not appear in other common honeys, and also finds this substance in acacia flower (see figure 2 Middle A), so it can be determined that the substance is derived from acacia. figure 2 Figure B in middle is the exact extracted ion current chromatogram (extraction window is 5mDa) of the compound found with higher content, its chromatographic retention time is 6.80min, and the exact mass number is m / z741.22366 in positive mode, such as image 3 As shown in panel A of , in addition, the exact mass of this substance in negative mode is m / z 739.20970 (as image 3 shown in panel C). image 3 Fi...

Embodiment 2

[0061] 1. Sample source

[0062] Purchase common different single flower honey samples directly from the bee farm to detect the content of acacia glycosides in various honeys, specifically including: jujube flower, acacia, rapeseed, litchi, longan, vitex, eucalyptus, snow lotus, sunflower, duck Footwood, tallow, pomegranate, wild osmanthus, wild dam, caragana, manuka, old melon head, wolfberry, codonopsis, gallnut, citrus honey. The number of honey samples of each variety is not less than 20; and the number of acacia honey samples is 154, which are collected from the main producing areas of acacia honey in my country in different years (2017-2020), such as Shaanxi, Shanxi, Gansu , Shandong, Hebei, Beijing, Liaoning and Henan provinces and cities.

[0063] 2. Solution preparation

[0064] Extraction solution: pipette 20mL of methanol, dilute to 100mL with ultrapure water, and store at 4°C.

[0065] Acacia glycoside standard solution: Weigh 10 mg of acacia glycoside standard s...

Embodiment 3

[0080] 1. Sample source

[0081] A total of 18 samples of various grades or brands of honey labeled as acacia honey were purchased from the market.

[0082] 2. Experimental steps

[0083] (1) Solution preparation

[0084] Extraction solution: pipette 20mL of methanol, dilute to 100mL with ultrapure water, and store at 4°C.

[0085] Acacia glycoside standard solution: Weigh 10 mg of acacia glycoside standard solution, and dilute to 10 mL with methanol. Store at 4°C, valid for 2 months.

[0086] (2) Sample processing

[0087] Weigh 0.8g honey and add 7.2mL extraction solution, vortex at room temperature until completely dissolved, and centrifuge at 20194g, 4°C for 20min. The supernatant was collected and passed through a 0.22 μm filter membrane, and then 100 μL of the extract: 900 μL of methanol water was diluted once. The diluent was transferred to a sample vial for analysis on the machine.

[0088] (3) Quality control samples

[0089] Weigh 0.8g of blank honey (without...

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Abstract

The invention belongs to the field of food detection, and particularly relates to an authenticity evaluation method of robinia pseudoacacia honey and application of the method in adulteration identification. According to the authenticity evaluation method, robinin is used as an indicating substance, and when the content of robinin in honey is 0.8-2.0 mg / kg, robinia pseudoacacia honey can be determined; and when the content of the robinin in the honey is lower than 0.8 mg / kg, the honey is judged as other honey or adulterated robinia pseudoacacia honey. The method provided by the invention has the advantages of simplicity, high efficiency and the like, is convenient to operate and popularize, has certain guiding significance for the formulation of related industries or national standards ofhigh-quality robinia pseudoacacia honey, and also has important practical significance for protecting legitimate rights and interests of honey consumers and maintaining the healthy development of thehoney consumption industry.

Description

technical field [0001] The invention relates to the field of food testing, in particular to a method for evaluating the authenticity of acacia honey and its application in adulteration identification. Background technique [0002] Acacia is a perennial deciduous tree of the Fabaceae Papilionaceae subfamily Robinia, also known as Robinia. Native to the Americas, it has been widely distributed all over the world because of its good drought tolerance and greening advantages. Introduced into my country at the end of the 19th century, it is now widely cultivated in the eastern and central regions of my country, such as the Liaodong Peninsula, North China Plain, Loess Plateau, Inner Mongolia, and Southwest China. Acacia is one of the main nectar-source plants in my country, especially the key nectar-source plants in spring in North China and Northwest China. Acacia honey, as one of the bulk commodity honeys in my country, is deeply loved by people because of its white color, sof...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N2030/324
Inventor 杨术鹏李熠张玉豪傅怡周金慧杨宇晖张金震
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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