The invention provides an access control method and system with a global anti-back-transmission function, comprising the following steps of: pre-storing an authorized card number, latest position information of the card number and a route map of the whole area in a controller; after receiving a legal door opening request and card swiping position information of a local logic rule, comparing the received position information of the card number with the stored latest position information of the card number to judge the overall anti-return transmission legality of the door opening request, and controlling a corresponding door to be opened after judging legality; and updating the latest position of the card number stored in the controller to be the target position to which the door opening request goes according to the route map of the whole area. By adopting the method and the system, whether the card swiping personnel violate the anti-back-transmission behavior rules can be directly judged by using hardware, the adverse effect of the software of the upper computer is eliminated, the unsafe factors such as the card swiping of the passers across defense areas, the card swiping and thecard access of multiple persons by using the same card and the like are effectively solved, and the facility safety is improved.