The invention discloses a three-phase decoupling load flow calculation method of a power distribution network based on a path matrix. The method comprises the following steps: firstly, adopting a symmetrical component method to perform sequence component decoupling on a three-phase unbalanced power distribution network to obtain zero sequence, a power distribution sequence network with positive sequence and negative sequence, and adopting a loop-analysis method based on the path matrix to perform one-phase-sequence component load flow calculation to obtain load flows of the three-sequence networks; and secondly, transforming sequence network load flows in a phase component mode by an inverse transformation principle of the symmetrical component method to obtain three-phase load flows. By using the method, a three-phase unbalanced power distribution network system is decoupled into zero sequence, positive sequence and negative sequence networks, so that large matrix manipulation in the three-phase load flow calculation is avoided, the calculated amount is decreased, and the calculation efficiency is improved. The method has the advantages of clear calculation process, simple programming and fast calculation speed. Finally, a 6-busbar test example verifies the correctness and good convergence; and the method is good in generality and practical applicability.