Method for identifying apis cerana honey and apis mellifera honey

A technology of honey and honey bees, applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of low yield, no production of propolis and royal jelly, low honey yield, etc., to achieve reasonable and simple design, accurate identification, Instrument common effects

Inactive Publication Date: 2016-05-04
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Apis cerana (abbreviated as Apis mellifera) is a subspecies of Apis mellifera. It is a unique bee species in China. It has the ability to use sporadic nectar source plants, strong collection ability, high utilization rate, long honey collection period and adaptability, and resistance to mite and disease. Strong ability, less feed consumption and other incomparable advantages of Italian bees, it is very suitable for fixed-point breeding in mountainous areas of China, but its honey production is low, and it does not produce propolis and royal jelly
[0005] Zhongfeng honey is also cal

Method used

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  • Method for identifying apis cerana honey and apis mellifera honey
  • Method for identifying apis cerana honey and apis mellifera honey
  • Method for identifying apis cerana honey and apis mellifera honey

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method for discriminating Chinese bee honey and Italian bee honey is realized through the following steps:

[0024] (1) Extraction of total protein in honey: Weigh 10g of Zhongfeng honey and Italian bee honey respectively, put each in a 50ml centrifuge tube, add 10ml of sterile water, shake and mix well, and centrifuge at 8000g / min at 4°C Centrifuge for 10 minutes to remove impurities such as beeswax and pollen. Carefully draw the supernatant into a new 50ml centrifuge tube with a pipette gun. Pre-cool the supernatant in a 4°C refrigerator for 10 minutes, then add it to a -20°C freezer for pre-cooling 20ml of absolute ethanol, stirred gently in an ice-water bath for 15 minutes, then centrifuged at a speed of 15000g / min for 10 minutes at 4°C, discarded the supernatant, added 1.5ml of sterile water to the precipitate, stirred with a glass rod, and then Shake and mix well, then divide into 1.5ml centrifuge tubes and store in -80°C refrigerator for later use. Honey from d...

Embodiment 2

[0032] In order to ensure the experimental effect, the raw honey in this example comes from several cooperative bee farms in Jinhua City, Zhejiang Province, and the sample information is shown in Table 1 below. The royal jelly samples of Chinese bee royal jelly and Italian bee royal jelly in this example came from the experimental apiary of Zhejiang University, and the sampling time was September 23, 2015. The royal jelly samples were stored in a -80°C refrigerator for later use. Take an appropriate amount of royal jelly sample in a 1.5ml centrifuge tube, dilute to an appropriate concentration with deionized water, and the subsequent treatment is the same as that of the honey protein sample.

[0033] Table 1 Experimental honey sample information table

[0034]

[0035] Carry out experiment according to the method step of embodiment one, band result sees figure 1 .

[0036] from figure 1 It can be clearly seen that the protein samples of Chinese bee honey have their own s...

Embodiment 3

[0038] In this embodiment, the commercial honey of Chinese and Italian bee honey is selected as a sample, and the experiment is carried out according to the method steps of Example 1. The results of protein electrophoresis showed that the target band existed stably in commercial honey, and it could have a good identification effect during the shelf life of commercial honey, and had a good market effect.

[0039] The commercial honey in this example is provided by a certain company. Among them, the Italian bee honey is acacia honey, and the Chinese bee honey is miscellaneous flower honey. The shelf life is 18 months. The detection time is December 8, 2015. The following table 2, the band results are shown in figure 2 , image 3 .

[0040] Table 2 Production batch number information table of commercial honey

[0041]

[0042] from figure 2 , image 3 It can be seen that with the prolongation of the shelf life, the protein components in commercial honey will gradually deg...

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Abstract

The invention provides a method for identifying apis cerana honey and apis mellifera honey, which achieves the purpose of identifying the apis cerana honey and the apis mellifera honey according to the specific band difference between the proteins of the apis cerana honey and the apis mellifera honey. After an apis cerana honey sample is treated, protein electrophoresis is conducted, the apis cerana honey has a 15.0KDa protein band, the apis mellifera honey has a 16.5KDa protein band, and a mixed honey sample of the both has both the 15.0KDa protein band and the 16.5KDa protein band. By differentiating the specific protein bands of the different honeys to achieve the purpose of identification, the method can accurately identify the apis cerana honey and the apis mellifera honey. In the method disclosed by the invention, the target proteins stably exist in the apis cerana honey and the apis mellifera honey, not affected by honey resources, concentration processing and shelf life. The design of the method disclosed by the invention is reasonable and simple, instruments are common, cost is low, identification is accurate, and the method is also suitable for identifying a certain proportion of apis mellifera honey mixed in the apis cerana honey.

Description

technical field [0001] The invention belongs to the field of bee products, and mainly relates to a method for identifying Chinese bee honey and Italian bee honey, which is to identify Chinese bee honey and Italian bee honey through biological methods. technical background [0002] Honey is a natural sweet substance that bees collect nectar, secretions or honeydew from plants, combine with their own secretions, transform, dehydrate, store and mature in the honeycomb. The secretions of bees in honey are mainly some proteins, including some digestive enzymes and royal jelly protein. [0003] Apis mellifera (abbreviated as Apis mellifera) is a subspecies of Apis mellifera. It is native to Italy and has a strong ability to produce honey and royal jelly. Can be used for propolis production. It is a bee species widely cultivated in the world, and it is also the main species of bee culture in China. [0004] Apis cerana (abbreviated as Apis mellifera) is a subspecies of Apis mell...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/447
Inventor 胡福良张言政郑火青张翠平
Owner ZHEJIANG UNIV
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