Construction method and identification application of fingerprint spectra of natural apis cerana honey of lychee flowers and natural apis mellifera honey of lychee flowers

A technology of fingerprint map and construction method, which is applied in the field of honey discrimination and detection, can solve the problems of inability to scientifically identify natural Chinese bee lychee honey and natural Italian bee lychee honey, lack of quality control indicators, and consumer trust crisis, and achieve high reliability. , strong uniqueness, small dosage effect

Active Publication Date: 2019-03-15
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Benefits of technology

This patented new way of finding out what kind of liquid called chlorophyllum amber acid (CAS) can cause damage when stored at higher temperatures than usual. It suggests searching through naturally occurring sources like royal jellyfish bone powder instead of expensive ones such as European ginseng. By comparing CAS levels found by analyzers against known standards, we are able to find ways to differentiate them more accurately.

Problems solved by technology

The technical problem addressed in this patents relates to identifying both types of lute honey during commercial manufacturing processes without being able to accurately determine their qualitative identity.

Method used

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  • Construction method and identification application of fingerprint spectra of natural apis cerana honey of lychee flowers and natural apis mellifera honey of lychee flowers
  • Construction method and identification application of fingerprint spectra of natural apis cerana honey of lychee flowers and natural apis mellifera honey of lychee flowers
  • Construction method and identification application of fingerprint spectra of natural apis cerana honey of lychee flowers and natural apis mellifera honey of lychee flowers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Construction of GC / MS fingerprints of natural Chinese bee lychee honey and natural Italian bee lychee honey

[0023] Put 20.00g of natural lychee honey samples and a magnetic stirrer into a 100mL headspace extraction bottle, adjust the rotation speed of the magnetic stirrer to 180rpm, mix well, keep at 70°C for 10min, and use a 50 / 30μm SPME extraction head (DVB / CAR / PDMS) at 70°C for 50 min; after the extraction, the SPME extraction head was taken out and quickly inserted into the injection port of the Agilent 7890BGC / 5977A MSD gas chromatography-mass spectrometer (GC-MS) for desorption for 5 min, and the GC Conditions: Agilent’s HP-5MS chromatographic column (30m×0.25mm×0.25μm) was used, the temperature of the injection port was 250°C, splitless injection, and the carrier gas was high-purity He (>99.999%); the temperature of the chromatographic column The program is: the initial temperature is 50°C, keep for 3min; 4°C / min to 130°C, keep for 5min; 10°C / min to...

Embodiment 2

[0037] Embodiment 2: the identification of natural lychee honey sample A to be tested

[0038] According to the construction method of the GC / MS fingerprint of the natural Chinese Italian bee lychee honey in Example 1, construct the volatile component total ion current chromatogram of the natural lychee honey sample A ( Figure 4 ), and the total ion chromatogram of the volatile components of natural lychee honey sample A was imported into the "Traditional Chinese Medicine Fingerprint Similarity Evaluation System (2012 Edition)", which was compared with the GC / MS fingerprint of natural lychee honey or natural lychee honey The GC / MS fingerprints of Italian bee lychee honey were matched to the peaks, and the similarity between them was calculated at the same time (Table 3). It can be seen from Table 3 that the similarity between the volatile component total ion chromatogram of the natural lychee honey sample A to be tested and the GC / MS fingerprints of the natural Chinese bee ly...

Embodiment 3

[0041] Embodiment 3: the identification of natural lychee honey sample B to be tested

[0042] According to the construction method of the GC / MS fingerprint of the natural Chinese Italian bee lychee honey in Example 1, construct the volatile component total ion current chromatogram of the natural lychee honey sample B to be measured ( Figure 5 ), and the total ion chromatogram of the volatile components of natural lychee honey sample B was imported into the "Traditional Chinese Medicine Fingerprint Similarity Evaluation System (2012 Edition)", which was compared with the GC of natural lychee honey or natural lychee honey / MS fingerprints for peak matching, and at the same time calculate its similarity with the two (Table 4). It can be seen from Table 4 that the similarity of the volatile component total ion chromatogram of the natural lychee honey sample B to be tested with the GC / MS fingerprints of the natural Chinese bee lychee honey and the natural Italian bee lychee honey...

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Abstract

The invention discloses a construction method and identification application of fingerprint spectra of natural apis cerana honey of lychee flowers and natural apis mellifera honey of lychee flowers. GC/MS fingerprint spectra of the natural apis cerana honey of lychee flowers and natural apis mellifera honey of lychee flowers are separately constructed by a headspace solid-phase microextraction-gaschromatography-mass spectrometry method and a traditional Chinese medicine fingerprint similarity evaluation system; and it determines that the GC/MS fingerprint spectrum of the natural apis cerana honey of lychee flowers contains 19 common feature volatile components, and the GC/MS fingerprint spectrum of the natural apis mellifera honey of lychee flowers contains 16 common feature volatile components. By comparison of similarity and chemical composition difference of a total ion chromatogram of a to-be-detected natural honey sample of lychee flowers and a constructed GC/MC fingerprint spectrum, the natural apis cerana honey of lychee flowers and the natural apis mellifera honey of lychee flowers are accurately distinguished and identified. The method has the characteristics of small useamount of the sample, high characteristics and reliable result, the problem that the natural apis cerana honey of lychee flowers and the natural apis mellifera honey of lychee flowers in markets aredifficult to distinguish is solved quite beneficially, and the social benefit is quite obvious.

Description

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Claims

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Application Information

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Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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