Method for constructing fingerprint spectrums of natural Apis cerana longan honey and natural A.mellifera ligustica longan honey and identification and application of fingerprint spectrums ofnatural Apis cerana longan honey and natural A.mellifera ligustica longan honey

Abstract

The invention discloses a method for constructingfingerprint spectrums of natural Apis cerana longan honey and natural A.mellifera ligustica longan honey and identification and application of the fingerprint spectrums of the natural Apis cerana longan honey and the natural A.mellifera ligustica longan honey. A headspace solid-phase microextraction-gas chromatography-mass spectrometry method and aChinese herbal fingerprint spectrum similarity evaluation system are used to respectively construct GC / MS fingerprint spectrums of the natural Apis cerana longan honey and the natural A.mellifera ligustica longan honey; the GC / MS fingerprint spectrum of the natural Apis cerana longan honey is determined to contain 16 common feature volatile ingredients, and the GC / MS fingerprint spectrum of thenatural A.mellifera ligustica longan honey contains 15 common feature volatile ingredients. By comparing the similarity and the difference of the chemical composition of a total ion chromatogram of a to-be-tested natural longan honey sample and the constructed GC / MS fingerprint spectrum, the natural Apis cerana longan honey and the natural A.mellifera ligustica longan honey are accurately distinguished and identified. The method has the characteristics of small use level of samples, strong characteristics and reliable result, is quite beneficial for solving the problem that the Apis cerana longanhoney and the A.mellifera ligustica longan honey are difficult to distinguish on the market, and has a quite remarkable social benefit.

Description

Technical field: [0001] The invention belongs to the field of distinguishing and detecting honey, and in particular relates to a method for constructing fingerprints of natural Chinese longan honey and natural Italian longan honey and their identification application. Background technique: [0002] The volatiles of nectar plant nectar are an important source of honey aroma substances. Honey from different nectar source plants or fake honey with different substances added often contain different volatile components (Vilma et al., 2008; Ren Jiamiao et al., 2016). The chromatographic fingerprint of the volatile components of honey refers to the chromatogram that can identify the differences between honey samples from different nectar plants or different regions (Konstantinosa et al., 2010; Jasicka-Misiak et al., 2018). This chromatogram is rich in polymorphism, and has the characteristics of high honey variety specificity and regional stability. For single-floral honey, due to...

AI Technical Summary

Problems solved by technology

However, due to the lack of standardized quality control indicators for natural longan honey, there are still many inferior or adulterated honeys in the market; (A.mellifera ligustica, Italian bee) honey is 3-5 times. Driven by profit, the market often uses natural Italian longan honey to mix or pretend to be natural Chinese bee longan honey. The processing enterprises of related bee products have brought great losses, and it is easy to cause a crisis of trust among consumers (Hu Fuliang et al., 2016)

At present, there is no relevant report on the use of GC / MS fingerprints of natural Chinese longan honey to distinguish natural Chinese longan honey from natural Chinese longan honey. Farmers make subjective distinctions. These methods mainly rely on experience and are highly subjective, so it is impossible to scientifically identify natural Chinese bee longan honey and natural Italian bee longan honey.

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