A construction method and identification application of fingerprints of natural Chinese bee longan honey and natural Italian bee longan honey

Abstract

The invention discloses a method for constructing fingerprints of natural Chinese bee longan honey and natural Italian bee longan honey and their identification application. The present invention obtains the GC / MS fingerprints of natural Chinese longan honey and natural Italian longan honey by adopting the method of headspace solid-phase microextraction-gas chromatography-mass spectrometry and the similarity evaluation system of traditional Chinese medicine fingerprints; The GC / MS fingerprint of Zhongfeng longan honey contains 16 common characteristic volatile components, and the GC / MS fingerprint of natural Italian longan honey contains 15 common characteristic volatile components. By comparing the similarity and chemical composition difference between the total ion chromatogram of the tested natural longan honey sample and the constructed GC / MS fingerprint, the natural Chinese bee longan honey and the natural Italian bee longan honey can be accurately distinguished. The method of the invention has the characteristics of small amount of samples, strong characteristics and reliable results, is very beneficial to solving the problem that the Chinese Italian bee longan honey is difficult to distinguish in the current market, and has very obvious social benefits.

Description

Technical field: [0001] The invention belongs to the field of distinguishing and detecting honey, and in particular relates to a method for constructing fingerprints of natural Chinese longan honey and natural Italian longan honey and their identification application. Background technique: [0002] The volatiles of nectar plant nectar are an important source of honey aroma substances. Honey from different nectar source plants or fake honey with different substances added often contain different volatile components (Vilma et al., 2008; Ren Jiamiao et al., 2016). The chromatographic fingerprint of the volatile components of honey refers to the chromatogram that can identify the differences between honey samples from different nectar plants or different regions (Konstantinosa et al., 2010; Jasicka-Misiak et al., 2018). This chromatogram is rich in polymorphism, and has the characteristics of high honey variety specificity and regional stability. For single-floral honey, due to...

AI Technical Summary

Problems solved by technology

However, due to the lack of standardized quality control indicators for natural longan honey, there are still many inferior or adulterated honeys in the market; (A.mellifera ligustica, Italian bee) honey is 3-5 times. Driven by profit, the market often uses natural Italian longan honey to mix or pretend to be natural Chinese bee longan honey. The processing enterprises of related bee products have brought great losses, and it is easy to cause a crisis of trust among consumers (Hu Fuliang et al., 2016)

At present, there is no relevant report on the use of GC / MS fingerprints of natural Chinese longan honey to distinguish natural Chinese longan honey from natural Chinese longan honey. Farmers make subjective distinctions. These methods mainly rely on experience and are highly subjective, so it is impossible to scientifically identify natural Chinese bee longan honey and natural Italian bee longan honey.

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