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A construction method and identification application of fingerprints of natural Chinese bee lychee honey and natural Italian bee lychee honey

A fingerprint and lychee honey technology, which is applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as the inability to scientifically identify natural Chinese bee lychee honey and natural Italian bee lychee honey, lack of quality control indicators, and consumer trust crisis. , to achieve the effect of high sensitivity, high reliability and strong uniqueness

Active Publication Date: 2021-11-23
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of standardized quality control indicators for natural lychee honey, there are still many inferior or adulterated honeys in the market; Honey bee (A. melliferaligustica, Italian bee) honey is 3-5 times. Driven by profit, it is often used in the market to use natural Italian bee lychee honey or pretend to be natural Chinese bee lychee honey. The processing enterprises of related bee products have brought great losses, and it is easy to cause a crisis of trust among consumers (Hu Fuliang et al., 2016)
At present, there is no relevant report on the GC / MS fingerprint of natural Chinese bee lychee honey at home and abroad. The distinction of natural Chinese bee lychee honey is mainly based on the subjective distinction of consumers or beekeepers. These methods mainly rely on experience and subjectivity. Large, it is impossible to scientifically identify natural Chinese bee lychee honey and natural Italian bee lychee honey

Method used

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  • A construction method and identification application of fingerprints of natural Chinese bee lychee honey and natural Italian bee lychee honey
  • A construction method and identification application of fingerprints of natural Chinese bee lychee honey and natural Italian bee lychee honey
  • A construction method and identification application of fingerprints of natural Chinese bee lychee honey and natural Italian bee lychee honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Construction of GC / MS fingerprints of natural Chinese bee lychee honey and natural Italian bee lychee honey

[0023] Put 20.00g of natural lychee honey samples and a magnetic stirrer into a 100mL headspace extraction bottle, adjust the rotation speed of the magnetic stirrer to 180rpm, mix well, keep at 70°C for 10min, and use a 50 / 30μm SPME extraction head (DVB / CAR / PDMS) at 70°C for 50 min; after the extraction, the SPME extraction head was taken out and quickly inserted into the injection port of the Agilent 7890BGC / 5977A MSD gas chromatography-mass spectrometer (GC-MS) for desorption for 5 min, and the GC Conditions: Agilent’s HP-5MS chromatographic column (30m×0.25mm×0.25μm) was used, the temperature of the injection port was 250°C, splitless injection, and the carrier gas was high-purity He (>99.999%); the temperature of the chromatographic column The program is: the initial temperature is 50°C, keep for 3min; 4°C / min to 130°C, keep for 5min; 10°C / min to...

Embodiment 2

[0037] Embodiment 2: the identification of natural lychee honey sample A to be tested

[0038] According to the construction method of the GC / MS fingerprint of the natural Chinese Italian bee lychee honey in Example 1, construct the volatile component total ion current chromatogram of the natural lychee honey sample A ( Figure 4 ), and the total ion chromatogram of the volatile components of natural lychee honey sample A was imported into the "Traditional Chinese Medicine Fingerprint Similarity Evaluation System (2012 Edition)", which was compared with the GC / MS fingerprint of natural lychee honey or natural lychee honey The GC / MS fingerprints of Italian bee lychee honey were matched to the peaks, and the similarity between them was calculated at the same time (Table 3). It can be seen from Table 3 that the similarity between the volatile component total ion chromatogram of the natural lychee honey sample A to be tested and the GC / MS fingerprints of the natural Chinese bee ly...

Embodiment 3

[0041] Embodiment 3: the identification of natural lychee honey sample B to be tested

[0042] According to the construction method of the GC / MS fingerprint of the natural Chinese Italian bee lychee honey in Example 1, construct the volatile component total ion current chromatogram of the natural lychee honey sample B to be measured ( Figure 5 ), and the total ion chromatogram of the volatile components of natural lychee honey sample B was imported into the "Traditional Chinese Medicine Fingerprint Similarity Evaluation System (2012 Edition)", which was compared with the GC of natural lychee honey or natural lychee honey / MS fingerprints for peak matching, and at the same time calculate its similarity with the two (Table 4). It can be seen from Table 4 that the similarity of the volatile component total ion chromatogram of the natural lychee honey sample B to be tested with the GC / MS fingerprints of the natural Chinese bee lychee honey and the natural Italian bee lychee honey...

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Abstract

The invention discloses a method for constructing fingerprints of natural Chinese bee lychee honey and natural Italian bee lychee honey and its identification application. The present invention obtains the GC / MS fingerprints of natural Chinese bee lychee honey and natural Italian bee lychee honey by adopting the method of headspace solid-phase microextraction-gas chromatography-mass spectrometry and the similarity evaluation system of traditional Chinese medicine fingerprints; The GC / MS fingerprint of lychee honey from Zhongfeng contains 19 common characteristic volatile components, and the GC / MS fingerprint of natural Italian lychee honey contains 16 common characteristic volatile components. By comparing the similarity and chemical composition difference between the total ion chromatogram of the tested natural lychee honey sample and the constructed GC / MS fingerprint, the natural Chinese bee lychee honey and natural Italian bee lychee honey can be accurately distinguished. The method of the invention has the characteristics of small amount of samples, strong characteristics and reliable results, is very beneficial to solving the problem that the lichee honey of Chinese Italian bee is difficult to distinguish in the current market, and has very obvious social benefits.

Description

Technical field: [0001] The invention belongs to the field of distinguishing and detecting honey, and in particular relates to a method for constructing fingerprints of natural Chinese bee lychee honey and natural Italian bee lychee honey and its identification application. Background technique: [0002] The volatiles of nectar plant nectar are an important source of honey aroma substances. Honey from different nectar source plants or fake honey with different substances added often contain different volatile components (Vilma et al., 2008; Ren Jiamiao et al., 2016). The chromatographic fingerprint of the volatile components of honey refers to the chromatogram that can identify the differences between honey samples from different nectar plants or different regions (Konstantinosa et al., 2010; Jasicka-Misiak et al., 2018). This chromatogram is rich in polymorphism, and has the characteristics of high honey variety specificity and regional stability. For single-floral honey, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 王华堂赵红霞黄文忠朱颖茵张学锋
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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