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A method for synthesizing bioplastic precursor polyhydroxyalkanoate by utilizing lignin-degrading bacteria

A technology of polyhydroxyalkanoate and lignin degrading bacteria is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of simple fermentation conditions and processes, and reduction of carbon sources and pretreatment costs.

Active Publication Date: 2019-10-25
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Alkali lignin carbon source without any pretreatment

Method used

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  • A method for synthesizing bioplastic precursor polyhydroxyalkanoate by utilizing lignin-degrading bacteria
  • A method for synthesizing bioplastic precursor polyhydroxyalkanoate by utilizing lignin-degrading bacteria
  • A method for synthesizing bioplastic precursor polyhydroxyalkanoate by utilizing lignin-degrading bacteria

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Embodiment 1

[0023] In the L-test tube, add 5 mL of LB medium, insert a single colony of Cupriavidus basilensis B-8 strain, and culture and grow at 30°C and 150 rpm until the optical density of the bacteria at 600 nm reaches 1.0. The pre-culture was centrifuged, washed, and then inoculated into Erlenmeyer flasks containing 100 mL of alkali lignin medium (1-6 g / L) of different concentrations, and cultured with shaking at 30°C and 150 rpm for 48 hours. Among them, the medium preparation method: alkali lignin 1 ~ 6g, (NH 4 ) 2 SO 4 0.28g, K 2 HPO 4 1g, MgSO 4 0.2g, CaCl 2 0.1g, FeSO 4 0.05g, MnSO 4 0.02g, KH 2 PO 4 1g, 15g agar, 1000mL distilled water, pH value is 7.0~7.4.

[0024] Collect a 2 mL sample, discard the supernatant, and resuspend the cells in 150 µL of deionized water and 50 µL of DMSO. Add 5 μL of Nile Red (0.15 mg / mL) to the suspension for staining for 30 min. Then observed with a fluorescence microscope, the results attached figure 1 As shown, it was confir...

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Abstract

The invention discloses the use of a lignin-degrading bacterium, and in particular relates to a method for synthesizing a bioplastic precursor—polyhydroxyalkanoate—by using a lignin-degrading bacterium (Cupriavidus basilensis B‑8, preservation number CGMCC No. 4240). The strain can use unpretreated lignin (medium lignin concentration 1-6g / L) as the sole carbon source for growth, and synthesize bioplastics under the condition of nitrogen deficiency (nitrogen concentration is less than 60mg / L) - Homopolymers of polyhydroxyalkanoates. The bacterial strain and the production method of the invention greatly reduce the cost of carbon source and its pretreatment, and have the potential of large-scale industrialization and industrialization.

Description

technical field [0001] The invention belongs to the technical field of preparing polyhydroxyalkanoate by microorganisms, and relates to the use of a strain of lignin-degrading bacteria, in particular to a method for synthesizing bioplastic precursor-polyhydroxyalkanoate by using lignin-degrading bacteria. Background technique [0002] Petrochemical plastics have become the most widely used material for human beings. In 2010, global plastic production reached nearly 3×10 8 t, which is expected to reach nearly 4×10 in 2050 8 t. However, the international oil reserves are decreasing year by year, and the environmental pollution of petrochemical plastics is gradually aggravating. Therefore, it is urgent to develop alternatives to new petrochemical plastics. Polyhydroxyalkanoate has similar mechanical properties to traditional plastics, and can be completely degraded by microorganisms and enter the natural ecological cycle. It is considered to be an important substitute for p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/62C12R1/01
CPCC12P7/625C12P2203/00C12N1/205C12R2001/01
Inventor 柴立元石岩颜旭刘明人杨志辉
Owner CENT SOUTH UNIV
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