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Cupriavidus strain capable of transforming heavy metals and application of strain

A technology of heavy metals and copper-loving bacteria, which is applied in the restoration of bacteria and polluted soil, and methods based on microorganisms, can solve the problem of unclear migration and transformation mechanism of arsenic, and achieve the effect of strong reducing effect and strong reducing effect.

Active Publication Date: 2016-03-16
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, it is still unclear how nitrate-dependent ferrous oxidizing bacteria play a role in mineralization in paddy soil, and the migration and transformation mechanism of arsenic in the whole microbial ferrous oxidation process

Method used

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  • Cupriavidus strain capable of transforming heavy metals and application of strain
  • Cupriavidus strain capable of transforming heavy metals and application of strain
  • Cupriavidus strain capable of transforming heavy metals and application of strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Enrichment and isolation of nitrate-dependent ferrous oxidizing bacteria Paddy-2.

[0020] Enrichment is to inoculate rice soil samples from Guangdong Province into liquid separation medium. The composition of each liter of liquid separation medium is: 20mM piperazine-1,4-diethanesulfonic acid (PIPES) buffer, 5.14mM NaCl, 1.03mMKH 2 PO 4 , 2.03mMMgCl 2 , 0.68mMCaCl 2 , 10.0ml trace elements and 10.0ml vitamin solution, and added with 5mM FeCl 2 4H 2 O, 10mM NaNO 3 and 5mMCH 3 COONa (solvent is deionized water), pH is adjusted to 7.0, wherein, vitamin solution contains 2.0mg biotin, 2.0mg folic acid, VB610.0mg vitamin, 5.0mg thiamine, 5.0mg riboflavin, 5.0 mg niacin, 5.0 mg calcium pantothenate, 0.1 mg vitamin B12, 5.0 mg p-aminobenzoic acid, 5.0 mg lipoic acid, dilute to 1 L with deionized water; the trace element solution contains 1.5 g nitrilotriacetic acid and 3.0 g MgSO per liter 4 ·7H 2 O, 0.5gMnSO 4 ·H 2 O, 1.0gNaCl, 0.1gFeSO 4 ·7H 2 O, 0.1...

Embodiment 2

[0028] Example 2: Iron oxidation process of strain Paddy-2.

[0029] The liquid medium formula used: every liter of liquid medium contains 20mMPIPES buffer, 5.14mMNaCl, 1.03mMKH 2 PO 4 , 2.03mMMgCl 2 , 0.68mMCaCl 2 , 5mMCH 3 Each of COONa, vitamin solution and trace element solution was 10.0mL (vitamin solution and trace element solution composition were the same as in Example 1), and the solvent was deionized water.

[0030] The bacteria lawn was picked from the slope of the preserved strain Paddy-2 and inoculated into the liquid medium, and the bacteria were activated on a shaker at 30°C and 180rpm for 18 hours, so that the number of bacteria reached the exponential growth phase. Centrifuge at 4°C and 6000rpm for 10min, remove the supernatant, resuspend with 20mMPIPES buffer, repeat the above operation twice, and suspend the precipitated bacterial suspension in the liquid medium to prepare the bacterial suspension.

[0031] Inoculate the bacterial suspension in the liqu...

Embodiment 3

[0034] Example 3: The action process of strain Paddy-2 on ferrous oxidation ore formation.

[0035] The preparation of liquid medium and the making of bacterial suspension are the same as in Example 2.

[0036] According to the result of embodiment 2, only under the condition that nitrate exists, bacterial strain just shows the ability of ferrous oxidation. Bacterial suspension was inoculated in liquid medium, and the experimental treatment was bacterial strain + nitrate + ferrous, and the culture solution without bacteria was used as a control to investigate the effect of bacterial strain on ferrous oxidation and mineralization.

[0037] The precipitate formed by ferrous oxidation was washed twice with phosphate buffer (10 mM, pH 7.0) under anaerobic conditions, freeze-dried, and tested. The attachment relationship between the formed precipitate and the bacterial strain was observed by transmission electron microscope (TEM).

[0038] Test results such as Figure 4 As shown...

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Abstract

The invention discloses a cupriavidus strain capable of transforming heavy metals and application of the strain. The strain is named as cupriavidus metallidurans Paddy-2, which is preserved in China General Microbiological Culture Collection Center of Institute of Microbiology of Chinese Academy of Sciences on No.3, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing on June 9, 2015, with preservation number of CGMCC No. 11028. The strain, which has a relatively strong ferrous oxidization capacity and is capable of achieving mineralization by ferrous oxidization in a nitrate environment, is applicable to soil improvement; and the strain also has a relatively strong reduction effect on arsenic and the reduction effect on the arsenic is stronger under a condition free from nitrate and ferrous ions; therefore, the strain is suitable for repairing arsenic-contaminated soil.

Description

technical field [0001] The invention belongs to the field of new strain screening technology and environmental microorganism technology, and in particular relates to a strain of the genus Coppercoronavirus capable of transforming heavy metals and an application thereof. Background technique [0002] Iron is the most important redox active element in red soil, and iron oxide is an important active mineral in soil. The main iron-containing minerals include goethite, hematite, lepidocite, ferrihydrite, pyrite and Shiwei Termanite and other forms, which are mainly wrapped in free iron oxide on the surface of soil particles, have high geochemical activity and have an important impact on the geochemical properties of various elements in the soil. Under anaerobic conditions, iron elements produce dissolved Fe(II) and Fe(II)-containing minerals under the action of iron-reducing bacteria or iron-oxidizing bacteria. These iron-containing minerals have surface charges, huge specific su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 李芳柏童辉陈鹏程刘承帅陈曼佳
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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