Pseudomonas putida with aerobic denitrifying capability and method of treating waste water by the same

A Pseudomonas putida, aerobic denitrification technology, applied in aerobic process treatment, chemical instruments and methods, microbial-based methods, etc., can solve problems such as anoxic denitrification

Inactive Publication Date: 2007-08-15
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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AI Technical Summary

Problems solved by technology

This strain has the characteristics of denitrification under aerobic conditions. It can be inoculated in conventional aerobic nitrification sludge to realize simultaneous nitrification and denitrification of nitrogen-containing wastewater, which can solve the needs of biological denitrification in traditional wastewater treatment. The problem of adopting anoxic denitrification and aerobic nitrification in stages

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  • Pseudomonas putida with aerobic denitrifying capability and method of treating waste water by the same

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Effect test

Embodiment 1

[0015] Embodiment 1: Determination method of aerobic denitrification performance of Pseudomonas putida (Pseudomonas putida) CGMCC No.1798

[0016] In order to prevent the introduction of miscellaneous bacteria into the simulated wastewater, both the simulated nitrate wastewater I and the simulated nitrate wastewater II were inactivated in a high-temperature sterilization pot at 0.1 MPa for 20 minutes. Get 5ml of sterilized nitrate simulated waste water I and put it into a test tube, insert the pure Pseudomonas putida (Pseudomonas putida) CGMCC No.1798 bacterial strain sample preserved with liquid paraffin seal. The test tube was placed in an air bath constant temperature shaker, the constant temperature was maintained at 30°C, and the shaker speed was 160r / min to activate the strain. After activating for 12 hours, inoculate 10% of the inoculum into a 250ml Erlenmeyer flask filled with 200ml nitrate simulated waste water II to measure denitrification and denitrification perform...

Embodiment 2-10

[0020] Example 2-10: Different experimental conditions and ranges determine the optimal denitrification conditions for Pseudomonas putida (Pseudomonas putida) CGMCC No.1798 strain

[0021]The present invention carries out 9 waste water treatment tests altogether, and the aerobic denitrification reaction conditions of Pseudomonas putida (Pseudomonas putida) WXZ-4CGMCC No.1798 bacterial strain are preferred.

[0022] The four reaction factors of wastewater aerobic denitrification are: dissolved oxygen concentration, which is realized by controlling different rotational speeds rpm. The three levels of rotational speed are 100, 160 and 220 rpm, and the corresponding dissolved oxygen concentrations are 4.2-5.6 mg / L ( 55~74% saturated dissolved oxygen), 6~7.2mg / L (79~95% saturated dissolved oxygen) and 7.0~7.4mg / L (92~95% saturated dissolved oxygen); the three levels of nitro nitrogen concentration are respectively 50, 100 and 200mg / L; the three levels of carbon-nitrogen ratio are 6...

Embodiment 11

[0026] Embodiment 11: PCR amplification and sequence determination of the 16SrRNA gene of Pseudomonas putida (Pseudomonas putida) CGMCC No.1798

[0027] Pseudomonas putida (Pseudomonas putida) CGMCC No.1798 was inoculated into 100 μL double-distilled water, boiled in a boiling water bath for 5 minutes, and colony PCR amplification was performed. The primers used for the 16S rRNAPCR reaction were a pair of universal primers, that is, the forward primer was f27 (5'-AGAGTTGATCCTGGCTCAG-3') and the reverse primer was r1492 (5'-GGTTACCTTGTTACGACTT-3'). Primers were synthesized by Beijing Huada Zhongsheng Technology Development Co., Ltd. PCR reaction system (50 μL): 5 μL of 10×Buffer, 4 μL of dNTPs, 1 μL of primers f27 and r1492, 39 μL of double distilled water, centrifuge and mix well, add 0.5 μL of DNA template, 0.5 μL of Taq enzyme. The PCR reaction program included: (1) pre-denaturation at 94°C for 5 min; (2) denaturation at 94°C for 1 min; (3) annealing at 50°C for 1 min; (4) ...

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Abstract

The invention discloses a pseudomonas putida (Pseudomonas putida) WXZ-4 CGMCC No.1798 strain with aerobic denitrifying property and a method to denitrify the waste water; which is characterized by the following: possessing strong tolerance degree for dissolved oxygen; deacidizing nitrate and nitrite with distinctive speed under the condition of 50-98% saturated dissolved oxygen; seeding to active sludge; proceeding enriched culture; realizing the aerobic anti-nitration.

Description

technical field [0001] The invention relates to a method for biological denitrification treatment of wastewater by adopting Pseudomonas putida WXZ-4 CGMCC No.1798 bacterial strain under aerobic conditions. The strain has strong tolerance to dissolved oxygen, and can use nitrate and nitrite as electron acceptors under the condition of 50-98% saturated dissolved oxygen. Nitrate and nitrite in sewage can be reduced to gaseous products in an aerobic environment to denitrify wastewater. Background technique [0002] Among the various denitrification methods that have been applied so far, biological denitrification is still the main method in wastewater denitrification treatment. The traditional wastewater biological denitrification treatment process is: the organic nitrogen compounds in the sewage are first converted into ammonia nitrogen in the biochemical process, and the ammonia nitrogen is converted into nitrate and nitrite by autotrophic nitrifying bacteria in the nitrifica...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/40
CPCY02W10/10
Inventor 汪苹翟茜项慕飞龙雯刘俊女杨志
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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