Composite mineral-leaching bacteria colony and use of same in biological metallurgy

A technology of biometallurgy and leaching bacteria, which is applied to compound leaching bacteria and its application fields, can solve problems such as the influence of microbial growth and reproduction, and achieve the effect of widening the scope of ore processing and high efficiency

Active Publication Date: 2009-09-23
CHINA NAT GOLD GROUP CORP TECH CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, mechanical agitation in the reactor produces a strong shear force on the le

Method used

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  • Composite mineral-leaching bacteria colony and use of same in biological metallurgy
  • Composite mineral-leaching bacteria colony and use of same in biological metallurgy
  • Composite mineral-leaching bacteria colony and use of same in biological metallurgy

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Experimental program
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Effect test

Example Embodiment

[0025] 2. Preparation of bacterial colonies

[0026] The composite flora of the present invention is screened by testers from acidic wastewater collected from a mining area in Yunnan. Firstly, the collected acidic wastewater is added to the aseptic reformed 9K culture medium and cultured in a constant temperature shaking incubator at 37°C. Carry out enrichment culture. After a period of time, observe under the microscope that there are a large number of motile bacteria, and then carry out adaptive culture of minerals. After the bacteria can grow in the minerals, conduct a mineral concentration gradient test on them to gradually increase the bacterial concentration. The slurry concentration of the group work, and finally industrial application after a long period of mineral directional acclimation.

[0027] 3. Identification method of flora

[0028] Establishment of 16S rDNA library and method of sequence analysis

[0029] a. Extraction of total DNA: refer to the method of La...

Example Embodiment

[0043] Embodiment 1. Utilize the strain of the present invention to invest in the construction of a bio-oxidation gold extraction plant in a gold mine in Liaoning Province. The factory adopts the mode of stirring oxidation, and utilizes the bacterial flora of the present invention to carry out biological pre-oxidation of sulfur-containing and arsenic-containing refractory gold ores. Afterwards, cyanide leaching is carried out. It is the first manufacturer in my country to successfully apply biological oxidation pretreatment technology in high latitude areas. The factory processes 150 tons of gold ore powder per day. The temperature and pH value of the bacterial flora can adapt to the range, and the arsenic ion tolerance is fully satisfied. Production demand, and further expansion on the original basis, the average gold leaching rate is over 96%.

[0044] Utilize the flora of the present invention, adopt the mode of stirring and oxidizing in the oxidation tank, carry out the oxida...

Example Embodiment

[0045] Example 2.

[0046] Using the bacterial flora of the present invention to carry out a gold extraction test by biological oxidation on a refractory gold mine in Liaoning, the leaching rate of gold is increased from 17.23% of direct leaching to 96.29%. The multi-element analysis of the ore is shown in the table below.

[0047] Multi-element analysis results

[0048] the element

[0049] Adopt the flora of the present invention and use the improved 9k culture medium as the nutrient substance of the flora, carry out biological oxidation pretreatment to this kind of ore, the concentration of the oxidized ore pulp is 20%, carry out the cyanide leaching of gold after stirring and oxidizing for 6 days, the leaching of gold The rate reaches 95.29%, and the leaching rate is relatively high. For specific extraction results, see Figure 8

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Abstract

The invention relates to a composite mineral-leaching bacteria colony and use of the same in biological metallurgy. The composite mineral-leaching bacteria colony has a preserving number of CGMCC No.2395 in the Common Micro-organism Center of China General Microbiological Culture Collection Center. The composite mineral-leaching bacteria colony has strong oxidative activity within a temperature range from 30 to 52 DEG C as well as a working pH value of between 0.8 and 2.25 and an arsenic ion tolerance of 0 to 20 g/L. The composite mineral-leaching bacteria colony is used for extracting and recovering gold or copper, and greatly expands the use range of biological metallurgy in gold mines. Meanwhile, due to the wide temperature adaptation range, the composite mineral-leaching bacteria colony improves the oxidation speed and reduces production cost. Therefore, the composite mineral-leaching bacteria colony has a significant application prospect in biological metallurgy industry.

Description

technical field [0001] The invention relates to a composite ore leaching bacterial group and its application. Background technique [0002] In refractory gold ores, because gold is wrapped by sulfide minerals, it presents a microscopic or submicroscopic state. The traditional recycling process not only has high production costs, high energy consumption, heavy environmental pollution, and low recovery rates. . Biometallurgy technology uses the action of acidophilic microorganisms to overcome the above disadvantages. There are many kinds of acidophilic bacteria, which can be divided into iron-oxidizing bacteria and sulfur-oxidizing bacteria. Most of the time they are mixed to act on sulfide minerals. Their optimum pH range is 1.5-2.0 In the process of biometallurgy, because bacteria oxidize sulfide minerals is an exothermic reaction, the temperature in the reactor is often higher than the external temperature, but it is still greatly affected by the external temperature. The...

Claims

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Application Information

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IPC IPC(8): C12N1/20C22B3/18C12S13/00C12R1/01
CPCY02P10/20
Inventor 高金昌金世斌郝福来杨凤韩晓光
Owner CHINA NAT GOLD GROUP CORP TECH CENT
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