Preparation of compost fermentation complex bacterial agent

A technology for fermenting composite bacteria and composting, which is applied in the field of preparation of composite microbial bacterial agents, which can solve the problems of complex operation, unsatisfactory deodorization effect, and heavy workload, and achieve cycle shortening, composting odor elimination, and degradation quality Enhanced effect

Active Publication Date: 2009-07-22
新疆保利兴农生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires two additions, the timing of the two additions needs to be mastered, and the stack needs to be turned once a day. The operation is complicated and the workload is large, and the deodorization effect is not ideal.

Method used

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  • Preparation of compost fermentation complex bacterial agent
  • Preparation of compost fermentation complex bacterial agent
  • Preparation of compost fermentation complex bacterial agent

Examples

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preparation example Construction

[0020] (1) Preparation of solid slope.

[0021] Lactobacillus plantarum slant medium formula: beef extract 5g, yeast extract 5g, peptone 10g, glucose 10g, lactose 5g, sodium chloride 5g, agar 15g, pH value 5.5-6.5, distilled water 1000ml.

[0022] Candida utilis adopts the formula of potato sugar agar medium: 200g of potatoes, 20g of glucose, 15-20g of agar, pH value of 7.2-7.4, and 1000ml of distilled water.

[0023] Bacillus subtilis slant medium formula: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar 15g, natural pH, distilled water 1000ml.

[0024] (2) Solid slant culture.

[0025] Candida utilis, Lactobacillus plantarum, and Bacillus subtilis were respectively connected to the solid inclined surface, wherein the culture temperature of Bacillus subtilis was 30-35°C, and the culture time was 16-24h; the culture of Candida utilis and Lactobacillus plantarum The temperature is 25-28°C, and the incubation time is 24-36h.

[0026] 2. Expand the cultiva...

Embodiment 1

[0035] Add 10g of sucrose, 0.5g of peptone, 1g of yeast powder, 0.5g of potassium dihydrogen phosphate, 1.0g of ammonium sulfate, 0.5g of calcium chloride, 0.36g of magnesium sulfate, 0.02g of ferrous sulfate, 1.0g of sodium acetate, and Sodium 0.5g, pH 7.0. The culture medium was sterilized at 115°C for 30 minutes. After mixing the seed liquids of Candida utilis, Lactobacillus plantarum and Bacillus subtilis at a volume ratio of 0.2:0.5:0.5, add 10% into the fermentation medium liquid after high-temperature sterilization, and statically Place the culture for 10 days; when the fermentation broth no longer produces bubbles, the fermentation is terminated. At this time, the pH value is 4.0, the taste is sour, and there are white membranes floating on the surface of the fermentation broth, and the number of living microorganisms reaches 1×10 9 Individuals / ml or more, the culture is complete.

Embodiment 2

[0037] Add 15g of sucrose, 2g of peptone, 2g of yeast powder, 0.5g of potassium dihydrogen phosphate, 1.0g of ammonium sulfate, 0.5g of calcium chloride, 0.5g of magnesium sulfate, 0.01g of ferrous sulfate, 1.0g of sodium acetate, and sodium bicarbonate in 1000ml of water 0.5g, pH 7.0. The culture medium was sterilized at 115°C for 30 minutes. After mixing the seed liquids of Candida utilis, Lactobacillus plantarum and Bacillus subtilis at a volume ratio of 0.5:1:0.8, insert them into the high-temperature sterilized fermentation medium in an amount of 5%, and statically place them at 30°C. Cultured for 7 days; when the fermentation broth no longer produces bubbles, the fermentation is terminated. At this time, the pH value is 3.8, the taste is sour, and there are white membranes floating on the surface of the fermentation broth. After shaking well, the microscopic count can reach 1×10 9 cells / ml, the culture is complete.

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Abstract

The invention discloses a preparation method of a combined microbial inoculum which is suitable for being inoculated in an early farmyard manure stage and is prepared by solid inclined plane cultivation of monomer strains, propagation cultivation of seed fluids and combined fermentation fluid cultivation that uses candida utilis, lactobacillus plantarum and bacillus subtilis. The number of active microorganisms can reach over 1 multiplied by 10 per milliliter. The combined microbial inoculum can be applied into farmyard manure processing of municipal refuse or high-temperature farmyard manure processing of poultry excrement. By the inoculation in the early farmyard manure stage, the reinforcement of early-stage acidification and substrate fungus activation as well as the implementation of quick through decomposition, the farmyard manure quality is improved and the farmyard manure production efficiency is raised.

Description

1. Technical field [0001] The invention relates to a production method of a microbial bacterial agent, in particular to a preparation method of a composite microbial bacterial agent for composting fermentation. 2. Background technology [0002] The high-temperature aerobic composting process is a commonly used technology for the resource treatment of solid waste. It is a biological treatment method that integrates harmless treatment and resource recycling. It has the advantages of strong material transformation ability and easy to be controlled by humans. The process starts from the raw material stage of the initial composting, successively goes through the heating stage, high temperature stage, cooling stage, ripening and stable stage, and finally reaches maturity. Since composting is a biological process, the populations of microorganisms that play a key role in the degradation of organic matter are different at different stages of the composting process. The various para...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C05F17/00C12R1/72C12R1/25C12R1/125
CPCY02W30/40
Inventor 葛春辉徐万里孙宁川张云舒钟新才邵华伟王伟于晶
Owner 新疆保利兴农生物有限公司
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