Method for preparing probiotic preparation for reducing blood lipid and adjusting intestinal flora

A technology for regulating intestinal flora and probiotic preparations, applied in the biological field, can solve problems such as liver and kidney damage, toxic side effects, blood lipid disorders, etc., achieve long shelf life, treatment and prevention, regulate intestinal flora, treat and The effect of preventing symptoms of hyperlipidemia

Inactive Publication Date: 2009-10-21
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the control of hyperlipidemia is mainly based on drugs containing lovastatin, which have serious side effects, such as myal

Method used

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  • Method for preparing probiotic preparation for reducing blood lipid and adjusting intestinal flora
  • Method for preparing probiotic preparation for reducing blood lipid and adjusting intestinal flora
  • Method for preparing probiotic preparation for reducing blood lipid and adjusting intestinal flora

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Embodiment Construction

[0019] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0020] 1. Screening and physiological function determination of Lactobacillus plantarum MA2 derived from Kefir

[0021] 1. Screening of Lactobacillus plantarum MA2 derived from Kefir:

[0022] (1) Screening of probiotic lactic acid bacteria in kefir grains:

[0023] Grind Tibetan kefir grains with a certain amount of normal saline in a sterilized mortar, dilute them appropriately with sterile normal saline, take 100 μL and spread them on MRS solid medium respectively, culture them anaerobically at 30°C for 48 hours, observe and Record the characteristics of the colonies and pick individual colonies of different shapes, and then streak and inoculate them on the MRS, repeat the purification of lactic acid bacteria several times to obtain p...

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Abstract

The invention relates to a method for preparing a probiotic preparation for reducing blood lipid and adjusting intestinal flora, which comprises the following steps that: (1) Kefir-source lactobacillus plantarum MA2 is subjected to high-density culture to obtain a fermentation fluid, and the thallus density of the fermentation fluid is not less than 10cfu/mL; (2) the fermentation fluid is subjected to centrifugal collection to obtain thalli, and the thalli are added with a protective agent and are freeze-dried into bacterial powder; and (3) the freeze-dried bacterial powder is added with an oligosaccharide mixture, and a finished product of the probiotic preparation can be obtained after even mixing. The prepared probiotic preparation has a function of reducing the blood lipid, also has a physiological function of adjusting the intestinal flora at the same time, can treat and prevent high blood lipid symptoms, adjust the intestinal flora, is non-toxic and safe, has long retention period, and can be prepared into capsules, micro-capsules, granules, tablets and the like.

Description

field of invention [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of a probiotic preparation for reducing blood fat and regulating intestinal flora. Background technique [0002] Cardiovascular disease is a serious health hazard. In recent years, with the progress of society, the improvement of people's living standards and the continuous acceleration of the pace of work, cardiovascular disease has gradually become the number one killer of human health. Cardiovascular diseases are related to elevated blood lipids, lack of exercise, excessive stress, family history of cardiovascular diseases, etc. Among them, elevated blood lipids are one of the main factors causing elevated cholesterol. At present, the control of hyperlipidemia is mainly based on drugs containing lovastatin, which have serious side effects, such as myalgia, headache, elevated transaminases, gastrointestinal discomfort, blood lipid disorders, liver and kidney ...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P1/00A61P3/06A61K31/733A61K31/716A61K31/715A61K31/702A61K35/747
Inventor 王艳萍许女
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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