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Heterotrophic nitrification microbial preparation, cultivation method and use thereof

A microbial agent, heterotrophic nitrification technology, applied in the field of environmental microorganisms, can solve the problems of high treatment cost, lengthy process, high COD, etc., and achieve the effect of high effective biological concentration, simple process, and fast denitrification

Active Publication Date: 2008-11-12
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Due to the characteristics of high COD and high ammonia nitrogen, aquaculture wastewater is treated by nitrification and denitrification denitrification process. The process is lengthy, the treatment cost is high and difficult to control, which is a major problem in the current environmental pollution control.

Method used

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  • Heterotrophic nitrification microbial preparation, cultivation method and use thereof
  • Heterotrophic nitrification microbial preparation, cultivation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: the immobilization of heterotrophic nitrifying microbial bacterial agent of the present invention

[0049] (1) The medium used:

[0050] Beef extract medium (g / L): beef extract 5.0, peptone 10.0, NaCl 5.0, adjust pH to 7.2-7.4, sterilize at 121°C for 20 minutes. If it is made into a solid medium, add 1.5% to 2.0% of agar.

[0051] (2) Culture conditions:

[0052] Use an inoculation loop to scrape a ring of bacteria from Stenotrophomonas maltophilia strain DN1.1 and Pseudomonasputida strain DN1.2 preserved on the slant of the beef extract medium, and inoculate it in the beef extract liquid medium, culture at 30-35°C, shake at 170-200rpm, and The bacterial liquid can be harvested within 20 to 24 hours, and the culture liquid of the heterotrophic nitrifying microorganism bacterial agent can be obtained.

[0053] (3) Put a total of 260 mL of the cultured heterotrophic nitrifying microorganism culture solution into a 4.5 L reactor without inoculating other a...

Embodiment 2

[0055] Embodiment 2: the treatment of pig farm wastewater by the heterotrophic nitrifying microbial bacterial agent of the present invention

[0056] Pig farm wastewater was taken from a pig farm in Chengdu, COD Cr 3180-4550mg / L, NH 4 + -N 455-600mg / L, pH 7.5-8.0.

[0057] Table 3 Heterotrophic nitrifying microorganism bacteria agent treatment pig farm wastewater test one

[0058]

[0059] Table 4 Heterotrophic nitrifying microorganism bacteria agent treatment pig farm wastewater test two

[0060]

[0061] The reactor is cylindrical, made of plexiglass, with a bottom diameter of 8cm, a height of 1.0m, an effective height of 90cm, and an effective volume of 4.5L. The 4 sampling ports are 60cm, 50cm, 40cm and 30cm away from the bottom in turn, and the mud discharge port is 10cm away from the bottom. Configure ACO-012 electromagnetic air compressor and microporous aeration head, LZB glass rotor gas flowmeter. The reactor operates in batch mode, and the results are sho...

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Abstract

The invention belongs to the environmental microorganism field, and in particular relates to a heterotrophic nitrification microorganism agent, a method for cultivating the same, and a use of the same in culture wastewater treatment. The microorganism agent contains Stenotrophomonas maltophilias strain DN1.1 and Pseudomonas putida strain DN1.2 which have the preservation registration numbers respectively as CCTCC M 207074 and CCTCC M 207075. The microorganism agent can effectively remove ammonia nitrogen and total nitrogen in a water body, accumulates no nitrite or nitrate during denitrification, and can simultaneously remove CODCr in organic wastewater, which is applicable to the treatment of high-concentration culture wastewater. The use of the microorganism agent in treating culture wastewater is simple in process and stable in effect.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a heterotrophic nitrifying microbial bacterial agent, a cultivation method thereof and an application in the treatment of aquaculture wastewater. Background technique [0002] Due to the characteristics of high COD and high ammonia nitrogen, aquaculture wastewater is treated by nitrification and denitrification denitrification process. The process is lengthy, the treatment cost is high and it is difficult to control. It is a major problem in the current environmental pollution control. Nitrification and denitrification denitrification process is a widely used biological denitrification technology, which is characterized in that the denitrification process is composed of two different types of microorganisms - autotrophic nitrifying bacteria and heterotrophic denitrifying bacteria through the coupling metabolism of nitrification and denitrification Convert a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/00C02F3/34C12R1/01C12R1/40C02F103/20
Inventor 黄钧李毅军
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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