Method for increasing yield of haematococcaceae astaxanthin with saccharose as carbon source through co-culture

A technology of Haematococcus astaxanthin and astaxanthin, which is applied in the field of Haematococcus astaxanthin yield, can solve problems such as poor utilization of sucrose by Haematococcus, and achieves reduction of culture cost, improvement of utilization rate, and improvement of The effect of yield

Inactive Publication Date: 2016-03-30
YANGZHOU UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the process of producing astaxanthin by cultivating Haematococcus with sucrose as carbon source, aiming at the problem that Haematococcus has poor ability to utilize sucrose, the present invention provides a method of improving Haematococcus with sucrose as carbon source through co-cultivation. Astaxanthin yield method, Haematococcus co-cultivated with Phaffia rhodozyma (Phaffiarhodozyma) which has the abil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Add 0.5g / L sucrose to BG11 medium, and sterilize;

[0023] (2) After the Haematococcus in the logarithmic growth phase was collected, it was added to the culture medium at a ratio of 5:1 and an initial biomass of 0.2g / L with Phaffia rhodozyme. Cultured for 4 days under the ventilation of 5vvm;

[0024] (3) At the later stage of cultivation, after 1 day of induction with ultraviolet light, the mixture of algae and bacteria was collected for the extraction of astaxanthin.

[0025] Processing effect test:

[0026] During the culture process, the yield of astaxanthin reaches 120mg / L / day, which is 30% higher than that under the pure culture of Haematococcus simplex using glucose as the carbon source.

Embodiment 2

[0028] (1) add waste molasses of 25g / L sucrose equivalent in SE medium, sterilize;

[0029] (2) After collecting Haematococcus pluvialis in the logarithmic growth phase, add Phaffia rhodozyme to the medium at a ratio of 20:1 and an initial biomass of 0.4g / L, at 15°C, pH8. Cultivate for 5 days under the ventilation of 0 and 1vvm;

[0030] (3) At the later stage of cultivation, after 2 days of high temperature induction, the mixture of algae and bacteria is collected for the extraction of astaxanthin.

[0031] Processing effect test:

[0032] During the culture process, the yield of astaxanthin reaches 80mg / L / day, which is 20% higher than that under the pure culture of Haematococcus simplex using glucose as the carbon source.

Embodiment 3

[0034] (1) add bagasse equivalent to 15g / L sucrose in Chu13 medium, and sterilize;

[0035] (2) After the Haematococcus in the logarithmic growth phase was collected, it was added to the culture medium with the ratio of 10:1 and the initial biomass of 0.1g / L to Phaffia rhodozyme. Cultured for 6 days under the ventilation of 20vvm;

[0036] (3) At the later stage of cultivation, after 3 days of induction with high light intensity, the mixture of algae and bacteria was collected for the extraction of astaxanthin.

[0037] Processing effect test:

[0038] During the culture process, the yield of astaxanthin reaches 100 mg / L / day, which is 25% higher than the pure culture of Haematococcus simplex with glucose as the carbon source.

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Abstract

The invention provides a method for increasing the yield of haematococcaceae astaxanthin with saccharose as a carbon source through co-culture. The method comprises the following steps that firstly, saccharose or a saccharose substitute is added in a conventional microalgae culture medium, and sterilization is carried out; secondly, cells of microalgae capable of generating astaxanthin in the ogarithmic growth period are selected and collected, and the cells of the microalgae and phaffia rhodozyma are mixed at the proportion of 20:1-1:1, inoculated into the microalgae culture medium and cultured under the ventilation condition; thirdly, in the late culture stage, an induction strategy is adopted for induction, and a mixture of microalgae bodies and phaffia rhodozyma thalli is collected and used for extracting astaxanthin. By means of the method, the haematococcaceae which can accumulate astaxanthin but is poor in saccharose utilization capability and the phaffia rhodozyma which has the extracellular sucrase secretion capability and can accumulate astaxanthin are co-cultured, the haematococcaceae can normally grow in the culture medium with saccharose as the carbon source and accumulate astaxanthin together with the phaffia rhodozyma, the problem that saccharose cannot be used by algae for producing astaxanthin is effectively solved, and the culture cost of astaxanthin is reduced.

Description

technical field [0001] The invention relates to a production method of astaxanthin, in particular to a method for increasing the yield of astaxanthin from Haematococcus algae with sucrose as a carbon source through co-cultivation, and belongs to the field of biotechnology. Background technique [0002] Astaxanthin is a natural bioactive substance and a carotenoid with strong antioxidant activity, so it is called "super antioxidant" and is widely used in food, medicine, health products, aquaculture And in cosmetics and other industries, it is estimated that the annual market value of astaxanthin in the world can reach 200 million US dollars, which has a very broad market prospect. Currently, astaxanthin on the market mainly relies on artificial synthesis. Compared with natural astaxanthin, artificial astaxanthin has obvious disadvantages in terms of structure, function, application and safety. Therefore, the natural astaxanthin Development and application have important econ...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P23/00C12N1/16C12N1/12C12R1/89C12R1/645
CPCC12P39/00C12N1/12C12N1/16C12P23/00
Inventor 王仕楷王娟
Owner YANGZHOU UNIV
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