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Phaffia rhodozyma YZUXHONG686 and application thereof

A technology of YZUXHONG686CCTCCNO.M208262 and Phaffia yeast, applied in the field of Phaffia yeast strains, can solve the problems of low astaxanthin content, no large-scale production, and multiple energy sources, and achieve the effect of simple nutritional requirements and cost reduction

Inactive Publication Date: 2009-11-18
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The industrial production of Phaffia yeast is limited mainly by the following reasons: the original strain has low astaxanthin content, and only produces about 300-500 μg / g CDW (Cell dry weight) of carotenoids; °C) fermentation, cooling is required in the production process, which consumes more energy; it is difficult to screen high-yielding strains with stable genetic traits
At present, only a few large foreign companies such as RedStar Co. and Igene Biotechnology Inc. can produce, and the domestic development of carotenoid products has been slow, and no large-scale production has been formed so far.

Method used

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  • Phaffia rhodozyma YZUXHONG686 and application thereof
  • Phaffia rhodozyma YZUXHONG686 and application thereof
  • Phaffia rhodozyma YZUXHONG686 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Screening of strains with stable and high-yielding astaxanthin genetic traits

[0031] 1. Physical mutagenesis

[0032] (1) Take the fresh bacteria that have been activated for 36-40 hours (the starting strain is Phaffia rhodozyma 2.1557, purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences) and shake it in a sterilized Erlenmeyer flask equipped with glass beads for 3 minutes. Clear, centrifuge and wash with normal saline to suspend, take 6mL of normal saline bacterial suspension (10 7 1 / mL) in a sterile petri dish, placed on a magnetic stirrer, placed at 20cm under a 30W ultraviolet lamp, after the ultraviolet lamp was preheated for 30 minutes, open the dish cover and magnetic stirrer, and after irradiating for 3 minutes, centrifuge to discard the normal saline. The culture solution was cultivated overnight, the bacterial solution was taken and centrifuged, suspended in normal saline, diluted appropriatel...

Embodiment 2

[0042] Example 2 Determination of Carotenoid and Astaxanthin Contents in Phaffia Yeast

[0043] 1. Cultivation of Phaffia yeast

[0044] (1) Inoculate one loop of the slant of Phaffia yeast YZUXHONG686 into a 250 mL Erlenmeyer flask containing 30 mL of culture solution, and incubate at 23° C. and 170 rpm for 48 hours.

[0045] (2) Inoculate 10% of the inoculum into a Erlenmeyer flask filled with 30 mL of culture solution, and incubate at 23° C. and 170 rpm for 192 hours.

[0046] 2. Determination of Carotenoid Content

[0047] Determination of dry cell weight: absorb 4 mL of fermentation broth, centrifuge at 5000 rpm for 5 min, wash once with an equal amount of distilled water, and dry at 80°C until constant weight.

[0048] Take 4 mL of the above fermentation broth and add 4 mL of dimethyl sulfoxide (DMSO) at 56 °C, shake for 10 min, add 4 mL of acetone for shaking for 2 min, add 4 mL of 20% NaCl solution and 8 mL of petroleum ether for shaking for 1 min, then centrifuge at...

Embodiment 3

[0055] Example 3 Effects of different carbon sources on the yield of astaxanthin from Phaffia yeast YZUXHONG686

[0056] In sugar-free basal medium ((NH 4 ) 2 SO 4 3g / L, MgSO 4 ·7H 2 O 1.5g / L, KH 2 PO 4 1.5g / L, yeast extract 1.5g / L, corn steep liquor 10g / L) were added the following carbon sources (g / L) based on the principle of equal carbon moles: glucose 35, sucrose 33.3, maltose 35, D-fructose 35, mannitol 35.4, sorbitol 35.4, D-arabinose 35, absolute ethanol 26.9, glycerol 35.9, cultured at 23°C and 170rpm for 8 days, and measured the content of carotenoids. The results showed that the content of carotenoids in each medium: sucrose was 48mg / L, glucose and fructose are both 45mg / L, from the perspective of industrial production cost, choose sucrose or glucose as the best carbon source.

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Abstract

The invention discloses Phaffia rhodozyma YZUXHONG686CCTCC NO.M 208262 and application thereof. The Phaffia rhodozyma YZUXHONG686CCTCC NO.M 208262 is used for producing astaxanthin, the nutritional condition is simple, medium components are used in industrial production, the process is simple and convenient, the yield of the astaxanthin is high, and the application facilitates the industrial production.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to a Phaffia strain and its application. Background technique [0002] Carotenoids are a class of hydrocarbons and their oxidized derivatives consisting of 8 isoprenoid units. Carotenoids are the most widely distributed type of pigments in nature, and they are also the most important pigments. They exist in the entire plant kingdom and in all photosynthetic cell tissues; they also exist in some bacteria, fungi, algae and animals. [0003] In recent years, the antioxidant properties of carotenoids have become more known and their demand has increased. According to the analysis report of the global market by the American Business Communication Corporation (BCC), the growth of the carotenoid market will increase at an annual rate of 2.9%, from 887 million US dollars in 2004 to 1 billion US dollars in 2009. More than 600 kinds of carotenoids have been isolated and identified from ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P23/00C12R1/645
Inventor 谢虹梁建生周元元胡建锋
Owner YANGZHOU UNIV
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