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Cosmetics based on phaffia rhodozyma raw materials and preparation method thereof

A technology of Phaffia rhodozyme and cosmetics, applied in the field of cosmetics and its preparation based on Phaffia rhodozyme raw materials, achieving high safety effects

Active Publication Date: 2016-03-30
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There is no research report on the simultaneous use of astaxanthin, yeast extract and dextran from Phaffia rhodozyme to prepare cosmetics that effectively scavenge free radicals

Method used

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  • Cosmetics based on phaffia rhodozyma raw materials and preparation method thereof
  • Cosmetics based on phaffia rhodozyma raw materials and preparation method thereof
  • Cosmetics based on phaffia rhodozyma raw materials and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] (1) The volume of the reaction system is 1L, containing 100g of Phaffia rhodozyma (ATCC66270) cells and 60mL of cellulase, the pH value is adjusted to about 4.5 with 3M hydrochloric acid, and the volume is constant with deionized water; React at ℃ for 6h, boil for 3min to inactivate the enzyme activity.

[0083] (2) After the reaction, centrifuge at 10,000 rpm for 10 min to wash the precipitate.

[0084] (3) Add 60 mL / g of edible ethanol to the yeast after enzymatic hydrolysis, shake and extract in the dark for 60 minutes on a shaking table with a rotation speed of 150 rpm, centrifuge at 10000 rpm for 10 minutes, collect the supernatant, and obtain an astaxanthin extraction rate of 91.93% (by The content of astaxanthin was determined by spectrophotometer, and the amount of astaxanthin extracted by the acid-heat treatment method was used as a contrast, that is, the astaxanthin extracted by the default hot acid method was the total amount of astaxanthin, and the extractio...

Embodiment 2

[0088] Screening of any component of the astaxanthin complex of embodiment 2

[0089] (1) Take 20g caprylic / capric triglyceride, 20g isononyl isononanoate, 20g isopropyl myristate, 20g olive oil, 20g squalane, 20g sweet almond oil and 20g glycerin.

[0090] (2) Add the carotenoids prepared in Example 1 to the oils in step (1) with the amount of astaxanthin being 6% (mass fraction, ie 1.2g) Mix by a high-speed, high-shear mixer in a dark environment.

[0091] (3) centrifuge at 5000rpm for 10min, carry out solid-liquid separation, take the liquid, and obtain the complex of astaxanthin.

[0092] (4) The absorbance of each astaxanthin-oil complex at 474 nm was measured by spectrophotometry to determine the solubility of each oil component to the pigment. Among them, caprylic acid / capric triglyceride has the largest dissolution of pigment, OD 474 =292.35; followed by isopropyl myristate, OD 474 =57.4; then isononyl isononanoate, OD 474 =19.52; the rest of the oils dissolve les...

Embodiment 3

[0094] Example 3 Determination of DPPH free radical scavenging ability of astaxanthin-caprylic acid / capric triglyceride complex

[0095] (1) Dilute the 539.4 mg / L astaxanthin-caprylic acid / capric triglyceride complex mother liquor with absolute ethanol until the astaxanthin content is 5.4, 7.2, 9.6, 12.8, 17.1, 22.76, 30.34, 40.46, 53.94mg / L.

[0096] (2) The DPPH free radical ability of the astaxanthin complex at each concentration was determined by the DPPH method for scavenging organic free radicals (Anarjan N, et al. Colloidalastaxanthin: Preparation, characterization and bioavailability evaluation, Food Chem. 2012, 135(3): 1303-1309.). Obtain the linear relationship between the content of astaxanthin and its DPPH free radical scavenging rate in this concentration range, the relational formula is: Y=1.397x+5.585, R 2 =0.992, wherein Y is DPPH free radical scavenging rate (%), X is astaxanthin concentration (mg / L). It can be known that the IC of astaxanthin-caprylic acid / ...

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Abstract

The invention discloses cosmetics based on phaffia rhodozyma raw materials and a preparation method thereof. The cosmetics contain astaxanthin coming from phaffia rhodozyma, or contain astaxanthin coming from phaffia rhodozyma, glucan coming from phaffia rhodozyma and yeast extract coming from phaffia rhodozyma. The raw materials used for the cosmetics come from phaffia rhodozyma, and the safety is high. Moreover, the astaxanthin, glucan and yeast extract which come from phaffia rhodozyma are matched to be used, so that the free radical scavenging capacity of the cosmetics can be further enhanced. The cosmetics are deep development and utilization of phaffia rhodozyma and are favorable for improving economic application value of phaffia rhodozyma.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to a cosmetic based on Phaffia rhodozyme raw materials and a preparation method thereof. Background technique [0002] Free radicals are an effective defense system of the body, but excessive free radicals will attack life macromolecules and various organelles, cause various damages to the body at the molecular level, cellular level, and tissue and organ level, and accelerate the aging process of the body and induce various diseases. Free radicals cause irreparable damage to skin cells, leading to skin aging; the damage of free radicals to cell membrane lipids is the deposition of lipofuscin, which causes spots on the skin; the damage of free radicals to epidermal nuclei can induce skin cancer. Human beings live in an environment where free radicals exist everywhere, and are constantly damaged by external free radicals. At the same time, human beings also continuously produce free radicals...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/73A61K8/35A61K8/31A61Q19/08A61Q19/00
CPCA61Q19/00A61Q19/08A61K8/31A61K8/35A61K8/73A61K8/99A61K2800/85
Inventor 朱明军周玲燕
Owner SOUTH CHINA UNIV OF TECH
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