High-yield natural astaxanthin fermentation method

Abstract

The invention discloses a high-yield natural astaxanthin fermentation method, which comprises the steps as follows: activating phaffia rhodozyma CGMCC6355 bacterial strains and preparing a seed liquid; inoculating the seed liquid to a fermentation culture medium to perform fermentation culture, and extracting astaxanthin after the end of the fermentation culture, wherein before the fermentation culture, adding an oxygen vector with a volume ratio of 0.5-6 percent to the fermentation culture medium; during the fermentation culture, adding a sugar source to enable the concentration of reducing sugar in a fermentation liquid to reach 2-3 percent when the concentration of the reducing sugar in the fermentation liquid is consumed to below 2 percent, and adding an astaxanthin precursor substance to the fermentation liquid every other 8-24 hours. The astaxanthin produced by adopting the method can be applied to high-grade feed additives for the aquaculture industry and the like, and has the effects on improving the egg laying rate of aquatic animals, boosting the growth, and resisting and preventing diseases. The high-yield natural astaxanthin fermentation method is low in cost, high in the utilization ratio of raw materials, simple to operate, and easy to achieve large-scale industrial production.

Description

technical field [0001] The invention relates to the field of microbial fermentation production, in particular to a fermentation method for high-yield natural astaxanthin. Background technique [0002] Astaxanthin (3,3′-dihydroxy-4,4′-diketone-β,β′-carotene) is a ketocarotenoid with strong antioxidant activity, and its antioxidant activity is 500 times that of vitamin E, it has a positive role in improving immunity, preventing the occurrence of chronic diseases such as tumors, cardiovascular diseases, and diabetes, and delaying aging. [0003] At present, synthetic astaxanthin costs 2,500 yuan per kilogram, and natural astaxanthin costs 7,000-10,000 yuan per kilogram. Due to the price difference, 97% of the market share is occupied by chemically synthesized astaxanthin. However, the chemical synthesis method is complex and requires multi-step chemical and biocatalytic reactions to complete, and the cost is high, and the synthesized astaxanthin is completely different from n...

AI Technical Summary

Problems solved by technology

The Chinese patent document with the publication number CN102864087A discloses a high-yielding Phaffia rhodozyme strain obtained after mutagenesis and fusion. The astaxanthin production of the strain is 14.3 times higher than that of the parent, but the astaxanthin production is still low. is 68mg / L, therefore, it is necessary to optimize the process conditions of fermentation

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