High-yield natural astaxanthin fermentation method

A technology of natural astaxanthin and fermentation method, which is applied in the field of microbial fermentation production, can solve the problems of low astaxanthin production, achieve the effects of increasing dissolved oxygen, promoting oxygen transfer, and reducing production costs

Active Publication Date: 2014-05-28
浙江皇冠科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The Chinese patent document with the publication number CN102864087A discloses a high-yielding Phaffia rhodozyme strain obtained after mutagenesis and fusion. The astaxanthin production of t

Method used

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  • High-yield natural astaxanthin fermentation method

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Comparison scheme
Effect test

Embodiment 1

[0056] The optimization of embodiment 1 astaxanthin fermentation conditions

[0057] 1. Optimization of oxygen carrier

[0058] (1) Inoculate the activated slant strain into a 250mL Erlenmeyer flask containing 30mL liquid YM medium, culture at 22±1°C, 180r / min for 20h; then inoculate 10% of the inoculum into a 100mL shake flask for fermentation In a 1000mL Erlenmeyer flask with base, culture at 22±1°C, 180r / min for 20h.

[0059] (2) The volume of the fermenter is 10 L, and the filling volume is 6 L. At the same time, oxygen carriers are added to the fermentation medium, and the fermentation is sterilized at 121° C. for 20 minutes. After cooling, inoculate the seed culture solution into the fermentation medium according to 10% inoculum amount for fermentation and cultivation. The cultivation temperature is 22±1°C, and the fermentation pH is controlled to be 5.0±0.5 by feeding 2mol / L NaOH and 2mol / L HCl, and the ventilation rate is 1.2 vvm, by adjusting the stirring speed to c...

Embodiment 2

[0105] The application of embodiment 2 natural astaxanthin

[0106] 1. Feed additives containing astaxanthin on egg production and hatching rate of snake snapping turtles and on the survival and growth of young turtles

[0107] (1)

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Abstract

The invention discloses a high-yield natural astaxanthin fermentation method, which comprises the steps as follows: activating phaffia rhodozyma CGMCC6355 bacterial strains and preparing a seed liquid; inoculating the seed liquid to a fermentation culture medium to perform fermentation culture, and extracting astaxanthin after the end of the fermentation culture, wherein before the fermentation culture, adding an oxygen vector with a volume ratio of 0.5-6 percent to the fermentation culture medium; during the fermentation culture, adding a sugar source to enable the concentration of reducing sugar in a fermentation liquid to reach 2-3 percent when the concentration of the reducing sugar in the fermentation liquid is consumed to below 2 percent, and adding an astaxanthin precursor substance to the fermentation liquid every other 8-24 hours. The astaxanthin produced by adopting the method can be applied to high-grade feed additives for the aquaculture industry and the like, and has the effects on improving the egg laying rate of aquatic animals, boosting the growth, and resisting and preventing diseases. The high-yield natural astaxanthin fermentation method is low in cost, high in the utilization ratio of raw materials, simple to operate, and easy to achieve large-scale industrial production.

Description

technical field [0001] The invention relates to the field of microbial fermentation production, in particular to a fermentation method for high-yield natural astaxanthin. Background technique [0002] Astaxanthin (3,3′-dihydroxy-4,4′-diketone-β,β′-carotene) is a ketocarotenoid with strong antioxidant activity, and its antioxidant activity is 500 times that of vitamin E, it has a positive role in improving immunity, preventing the occurrence of chronic diseases such as tumors, cardiovascular diseases, and diabetes, and delaying aging. [0003] At present, synthetic astaxanthin costs 2,500 yuan per kilogram, and natural astaxanthin costs 7,000-10,000 yuan per kilogram. Due to the price difference, 97% of the market share is occupied by chemically synthesized astaxanthin. However, the chemical synthesis method is complex and requires multi-step chemical and biocatalytic reactions to complete, and the cost is high, and the synthesized astaxanthin is completely different from n...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
Inventor 胡向东潘玲燕叶茂胡伟卿章祺梁新乐
Owner 浙江皇冠科技有限公司
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