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Method for extracting phaffia rhodozyma intracellular astaxanthin

A technology of Phaffia rhodozyma and extraction method, which is applied in the field of intracellular astaxanthin extraction of Phaffia rhodozyme, can solve the problems of low efficiency of natural astaxanthin, unstable raw materials, low extraction rate of astaxanthin, etc., and achieve reduction The effect of generating industrial wastewater, simplifying the removal process, and improving the utilization rate of raw materials

Inactive Publication Date: 2016-07-06
XIAMEN HUISON BIOTECH
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Problems solved by technology

There are two production methods for natural astaxanthin: one is to extract from aquatic waste (shrimp, crab shells, etc.), which has disadvantages such as unstable raw materials, low extraction rate of astaxanthin, and complicated extraction process; the other is Microbial (Haematococcus pluvialis, Phaffia, etc.) fermented production, the content of astaxanthin in Haematococcus pluvialls cells is high (up to 0.2%~2.0% of dry cell weight), but the culture period is long (can be more than 15 days), low biomass (dry cell weight generally not more than 3g / L), and it is difficult to extract astaxanthin by breaking the algae cell wall. Therefore, the efficiency of natural astaxanthin production by Haematococcus pluvialis is low

Method used

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Embodiment Construction

[0025] The invention discloses a method for extracting astaxanthin from Phaffia rhodozyma intracellularly, comprising the following steps:

[0026] Step (1) Concentrating the fermentation broth: concentrating the fermentation broth of Phaffia rhodozyme to obtain the sludge;

[0027] Step (2) Bacteria inactivation: heat up the temperature of the bacteria sludge to 72°C for 30 minutes, then add citric acid to adjust the pH value to 5.5;

[0028] Step (3) Phaffia rhodozyma cell wall breaking: Homogenize in a high-pressure homogenizer at 90 MPa for 2 times to break the cell wall, and then perform sand milling to break the emulsion;

[0029] Step (4) Rough extraction of astaxanthin with ethanol / acetic acid mixture: after the cell wall is broken, use ethanol / acetic acid (v:v=1:1) mixture to extract astaxanthin at a material-to-liquid ratio of 1:15. The liquid ratio is the ratio of the mass of Phaffia spp. to the volume of the ethanol / acetic acid mixed solution, i.e. g / ml, extracted...

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Abstract

The invention discloses a method for extracting astaxanthin from Phaffia rhodozyma cells. Firstly, the fermented liquid of Phaffia rhodozyme is concentrated to obtain bacteria sludge; Astaxanthin is extracted with a mixed solution of acetic acid, and then back-extracted with oil. The mixed phase is shaken and mixed at a constant temperature. After centrifugal separation, the upper oil phase is collected to obtain an astaxanthin oil product rich in astaxanthin. The method has simple process, is easy to operate and control, and is suitable for industrial production of astaxanthin.

Description

technical field [0001] The invention relates to the technical field of astaxanthin, in particular to a technique for extracting intracellular astaxanthin from Phaffia rhodozyma. Background technique [0002] Astaxanthin is a non-vitamin A carotenoid with strong antioxidant and coloring ability, and is widely used in food, medicine, feed, cosmetics, health products and other fields. In recent years, it has attracted great attention because of its remarkable effect in the prevention and treatment of many diseases (such as cardiovascular and cerebrovascular diseases, Parkinson's syndrome, etc.). [0003] According to the source, astaxanthin can be divided into chemically synthesized astaxanthin and natural astaxanthin. The chemical synthesis of astaxanthin has mature technology and low production cost, but the biological activity of the synthesized astaxanthin is significantly lower, and there is a certain residual toxicity. The FDA (US Food and Drug Administration) only appro...

Claims

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Application Information

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IPC IPC(8): C07C403/24
CPCC07C403/24
Inventor 陈礼毅钟惠昌陈水荣
Owner XIAMEN HUISON BIOTECH
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