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Method for preparing astaxanthin feed additive by using bear spent grains

A feed additive, astaxanthin technology, applied in animal feed, animal feed, applications, etc., can solve problems such as inability to realize industrialization, and achieve the effect of high astaxanthin yield

Inactive Publication Date: 2010-05-12
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cost is a major obstacle for the production of astaxanthin feed additive by Phaffia rhodozyme fermentation. At present, the production of astaxanthin feed additive by fermentation of Phaffia rhodozyme in my country has not yet achieved industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for preparing an astaxanthin feed additive by utilizing brewer's grains, the specific process is as follows:

[0030] (1) 1.0 kilograms of beer wet wheat grains are wet-milled by a refiner to obtain a coarse slurry;

[0031] (2) After adding water to adjust the solid-to-liquid ratio of the above coarse slurry to 1:10, adjust the pH value to 5.5, put it into a 5L fermenter, sterilize at 121°C for 60 minutes, and cool to room temperature;

[0032] (3) Add 20 FPU of cellulase (Spezyme CP, purchased from Genencor-Danisco) per gram of solid matter (dry weight), control the temperature at 50°C, and stir at a speed of 150rpm to carry out the enzymatic hydrolysis reaction. The time is 12 hours, and the enzymolysis solution is obtained;

[0033] (4) Phaffia rhodozyma (ATCC 66270) stored at 4°C was amplified on a test tube slope and a shaker flask, and the number of cells obtained was greater than 10 6 A / mL yeast seed solution; the seed medium adopts YPD medium, the ...

Embodiment 2

[0037] The method for preparing an astaxanthin feed additive by utilizing brewer's grains, the specific process is as follows:

[0038] (1) Pretreatment of beer grains: 0.8 kg of beer wet grains are wet-milled by a refiner to obtain a coarse slurry;

[0039] (2) Sterilization: After adjusting the above coarse slurry according to the solid-to-liquid ratio of 1:12, adjust the pH value to 4.0, put it into a 5L fermenter, sterilize at 121°C for 60 minutes, and cool to room temperature;

[0040] (3) Enzymolysis: add 10 FPU of cellulase (Spezyme CP, purchased from Genencor-Danisco) per gram of solid matter (dry), control the temperature at 45°C, and stir at 150rpm for enzymolysis Reaction, the reaction time is 48 hours, and the enzymolysis solution is obtained;

[0041] (4) Preparation of Phaffia rhodozyma seed solution: Phaffia rhodozyma (ATCC 66270) stored at -80°C was amplified on a test tube slope and a shaker flask, and the number of cells obtained was greater than 10 6 A / mL ...

Embodiment 3

[0045] The method for preparing an astaxanthin feed additive by utilizing brewer's grains, the specific process is as follows:

[0046] (1) Pretreatment of beer grains: 0.7 kilograms of beer wet grains are wet-milled by a refiner to obtain coarse slurry;

[0047] (2) Sterilization: After adjusting the above coarse slurry according to the solid-to-liquid ratio of 1:15, adjust the pH value to 5.0, put it into a 5L fermenter, sterilize at 121°C for 60 minutes, and cool to room temperature;

[0048] (3) Enzymolysis: add 4 FPU cellulase (Spezyme CP, purchased from Genencor-Danisco) per gram of solid matter (dry), control the temperature at 40°C, and stir at a speed of 150rpm to carry out the enzymolysis reaction , the reaction time is 72 hours, and the enzymolysis solution is obtained;

[0049] (4) Preparation of Phaffia rhodozyma seed solution: Phaffia rhodozyma (ATCC66270) stored at -196°C was amplified by test tube slant and shake flask, and the number of cells obtained was mor...

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Abstract

The invention discloses a method for preparing an astaxanthin feed additive by using bear spent grains, which comprises the steps of pretreatment of the bear spent grains, enzymolysis of the bear spent grains, sterilization, preparation of a seed liquid of phaffia rhodozyma, fermentation culture of the phaffia rhodozyma, drying and the like. The method has the characteristics of high utilization ratio, good product quality and easy industrialization, realizes the production of the astaxanthin feed additive by industrial wastes, namely the bear spent grains, and achieves the aims of resource utilization and high value of the wastes.

Description

technical field [0001] The invention belongs to the field of microbial fermentation production, and in particular relates to a method for preparing astaxanthin feed additive by using brewer's grains. Background technique [0002] Beer grains are the main by-products of the beer industry (accounting for more than 80% of the by-products), and its protein content is 23%-30% (by dry weight), which is a good protein resource. So far it has been mainly sold cheaply as feed. However, due to the high content of crude fiber in brewer's grains and the poor balance of amino acids in protein, its feeding value is very low. Saccharification of crude fiber followed by fermentation to prepare high-protein feed is the direction of comprehensive utilization of brewer's grains. In addition to cellulose, the fiber in malt grains also contains a large amount of hemicellulose. How to utilize the hemicellulose in grain grains or its hydrolyzate (mainly xylose) is also a problem worthy of attent...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/06A23K1/18A23K10/14
Inventor 朱明军王菊芳吴振强
Owner SOUTH CHINA UNIV OF TECH
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