Method of separating and purifying astaxanthin from Phaffia rhodozyma

A technology for separation and purification of Phaffia yeast, applied in the field of astaxanthin, which can solve the problems of loss of effective activity and achieve the effect of maintaining quality, less impurities and maintaining stability

Active Publication Date: 2014-06-11
JIMEI UNIV
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Problems solved by technology

In the article, this method also uses column chromatography to purify astaxanthin, which also causes the loss of its effective activity for astaxanthin
[0006] The invention of the combination of high-efficiency extraction and simple separation and purification of astaxanthin in Phaffia yeast has not yet been reported. Therefore, how to efficiently extract astaxanthin, adopt simple separation and purification steps, and avoid column chromatography is a matter for those skilled in the art. The focus of the research

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  • Method of separating and purifying astaxanthin from Phaffia rhodozyma
  • Method of separating and purifying astaxanthin from Phaffia rhodozyma
  • Method of separating and purifying astaxanthin from Phaffia rhodozyma

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Embodiment Construction

[0024] The present invention will be further described below with reference to the drawings and specific embodiments.

[0025] The present invention discloses a method for separating and purifying astaxanthin from Phaffia yeast, which includes the following steps:

[0026] Step 1. Breaking the Phaffia cell walls: use dimethyl sulfoxide to break the Phaffia cell walls, and the conditions for breaking the walls are: the ratio of the cells to the dimethyl sulfoxide in terms of mass and volume, ie g / ml, It is 1 / 2, and the wall is broken for 5 minutes at 55℃; the use of dimethyl sulfoxide has high wall breaking rate and good extraction effect; short treatment time (5min) and low treatment temperature (55℃) are beneficial to astaxanthin Structural stability;

[0027] Step 2. Crude astaxanthin extraction with acetone: After breaking the wall, extract with acetone at a material-to-liquid ratio of 1:15. The material-to-liquid ratio is the mass-to-volume ratio of Phaffia yeast cells and aceto...

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Abstract

The invention discloses a method of separating and purifying astaxanthin from Phaffia rhodozyma. The method comprises the following steps: dilapidating walls of Phaffia rhodozyma thalli; coarsely extracting astaxanthin by use of acetone; degreasing to remove low-polarity carotenoid; removing dimethyl sulfoxide; crystallizing and the like. The purpose of quickly separating astaxanthin is realized by combining efficient extraction by an organic solvent and simple separation and purification step. The prepared astaxanthin is high in purity and fewer in impurity. The conventional column chromatography step is avoided, and the active functional groups of astaxanthin can be effectively maintained so as to maintain the stability of product quality of astaxanthin in the separation and purification step.

Description

technical field [0001] The invention relates to the technical field of astaxanthin, in particular to a method for separating and purifying astaxanthin from Phaffia yeast. Background technique [0002] The chemical name of astaxanthin is: 3,3'-dihydroxy-β,β'-carotene-4,4'diketone, which is a nutritional pigment and antioxidant with great development potential. Anti-oxidation, anti-aging, anti-virus, anti-body coloring, anti-cancer and many other important biological functions, it has broad application prospects in food, medicine, cosmetics, aquaculture, livestock and poultry breeding, chemical industry and other industries. [0003] The production methods of astaxanthin can be divided into chemical synthesis and biosynthesis. At present, chemical synthesis of astaxanthin has a mature process and low production cost, but compared with natural astaxanthin, the biological activity of chemically synthesized astaxanthin is significantly lower. Phaffia yeast has attracted much at...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
Inventor 杜希萍倪辉黄高凌杨远帆李利君肖安风蔡慧农
Owner JIMEI UNIV
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