Method for improving Phaffia rhodozyma strain high-yield astaxanthin

A technology of yeast strain and astaxanthin, which is applied in the field of fermentation, can solve the problems of astaxanthin yield to be improved, and achieve the effect of reducing fermentation cost and increasing yield

Pending Publication Date: 2017-05-24
浙江皇冠科技有限公司
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AI Technical Summary

Problems solved by technology

This prior art satisfies low cost and is suitable for industrial production, but the yield of astaxanthin still needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Improve the method for astaxanthin produced by Phaffia rhodozyma strain, which comprises the following steps:

[0019] Phaffia rhodozyma strain CZ10 was deposited on July 25, 2012 at the General Microbiology Center of the Chinese Academy of Microbiology, Institute of Microbiology, Chinese Academy of Sciences, No. CGMCC No. 6355.

[0020] 1) Inoculate the activated Phaffia rhodozyma strain at 5~12% inoculum in medium shake flask culture medium at 20~27°C, with ventilation at 1.0~1.2vvm, stirring at 100~200r / min, and expand culture for 20~ 35h;

[0021] 2) Inoculate the expanded Phaffia rhodozyma strain into a fermenter with a fermentation medium at an inoculum size of 6-10%, and add ammonium sulfate as a nitrogen source to make the C / N ratio 2;

[0022] 3) Add oxygen carrier to the fermenter. According to the volume ratio, the amount of oxygen carrier added is 0.5~5% of the volume percentage of the fermentation medium, the culture temperature is 20~27°C, the stirring s...

Embodiment 2

[0027] Shake flask medium: 5% peptone, 5% beef extract, 6% NaCl, 0.01% MnSO 4 •H 2 O, 8% agar, 0.01% isooctyl alcohol and the rest of sterile water; 79 parts of agricultural and sideline product pretreatment liquid, 26 parts of soybean meal, 13 parts of glucose, 6 parts of peptone, 6 parts of concentrated yeast extract powder, CaCl 2 2H 2 O 0.3 parts, MgSO 4 ·7H 2 O3 parts, KH 2 PO 4 1 part, (NH 4 ) 2 SO 4 3 parts, 1 part of ethanol, 0.01 part of triethanolamine;

[0028] Fermentation medium: 79 parts of agricultural and sideline product pretreatment liquid, 26 parts of soybean meal, 13 parts of glucose, 6 parts of peptone, 6 parts of concentrated yeast extract powder, CaCl 2 2H 2 O 0.3 parts, MgSO 4 ·7H 2 O3 parts, KH 2 PO 4 1 part, (NH 4 ) 2 SO 4 3 parts, 1 part of ethanol, 0.01 part of triethanolamine;

[0029] Nutrient solution:: 1% (NH 4 ) 2 · SO 4 , 0.1% cellobiose, 0.3% KH 2 PO 4 , 0.15% MnSO 4 , 0.01% s-triazine and the rest sterile water.

[0...

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Abstract

The invention provides a method for improving Phaffia rhodozyma strain high-yield astaxanthin. The method comprises the steps of multiplication culture and fermentation and separation, wherein a fermentation culture medium is prepared from, by weight, 75-85 parts of an agricultural and sideline product pretreatment solution, 20-30 parts of soybean meal, 8-14 parts of glucose, 4-8 parts of peptone, 4-8 parts of concentrated yeast extract powder, 0.2-0.4 part of CaCl2.2H2O, 1-4 parts of MgSO4.7H2O, 1-2 parts of KH2PO4, 1-5 parts of (NH4)2SO4, 1-3 parts of ethanol and 0.01-1 part of triethanolamine. According to the method, while the strain is stimulated to produce the astaxanthin, synthesis of the astaxanthin is promoted in the fermentation process, and therefore the astaxanthin yield is obviously increased, the fermentation cost is lowered, resources are fully utilized, triethanolamine promotes synthesis of the astaxanthin, and then the yield is obviously increased.

Description

technical field [0001] The invention belongs to the field of fermentation, and in particular relates to a method for improving the high-yield astaxanthin of Phaffia rhodozyme strains. [0002] technical background [0003] Astaxanthin (3,3′-dihydroxy-4,4′diketo-β, β′carotene) is a ketocarotenoid with a natural red color. Astaxanthin was originally used as a feed additive in aquaculture. Later pharmacological and physiological studies showed that astaxanthin has strong anti-oxidation and free radical quenching functions, and can promote the production of antibodies and enhance the immune function of the host. It has broad application prospects in the fields of food, medicine and cosmetics, and has high economic value. [0004] Phaffia rhodozyma is the only strain that can naturally produce astaxanthin, and its trans-astaxanthin was approved by FDA in 2000 for food additives. It is the only species in the kingdom Fungi, Phylum Fungi, Subphylum Deuteromycota, Cryptococcus fami...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
CPCC12P23/00
Inventor 胡向东叶茂胡伟卿梁新乐
Owner 浙江皇冠科技有限公司
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