Phaffia rhodozyma with high output of lycopene and production method of lycopene

Abstract

The invention discloses phaffia rhodozyma. The collection number of the phaffia rhodozyma is CCTCC M 2019083. A production method of the lycopene comprises the following steps of (1) selecting the phaffia rhodozyma, activating, inoculating into a fermenting culture medium, and culturing in a bed shaking way, so as to obtain a fermenting solution; (2) centrifuging the fermenting solution, collecting precipitate to obtain strain, washing by water, centrifuging, removing supernatant, adding 3mol/L of hydrochloric acid solution, uniformly oscillating, soaking, boiling in water bath, cooling, centrifuging, collecting precipitate, washing by water, centrifuging, and removing supernatant, so as to obtain cell fragments; (3) adding acetone, oscillating and extracting under the light shading condition, centrifuging, and collecting supernatant; (4) relieving pressure and evaporating, adding methanol, obtaining a methanol lycopene solution, transferring into a chromatographic column, eluting by methanol, collecting the eluting solution, relieving pressure and evaporating, so as to obtain the lycopene; preparing the dry strain with lycopene content of 30.32mg/g.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a Phaffia rhodozyme strain with high lycopene production and a lycopene production method. Background technique [0002] Lycopene, also known as ψ-carotene, molecular formula C 40 h 56 , with a relative molecular mass of 536.8, composed of 11 conjugated double bonds and 2 non-conjugated double bonds. It is orange-yellow in a lower concentration solution, usually dark red powder or oily liquid, and pure lycopene is needle-like dark red, insoluble in water, difficult to dissolve in polar organic solvents, soluble in low polar organic solvents Organic solvents, so less polar organic solvents are usually used to extract lycopene. Lycopene is a terpenoid with strong antioxidant activity. It has various physiological functions such as quenching singlet oxygen, scavenging free radicals, and inhibiting lipid peroxidation. Its ability to quench singlet oxygen...

AI Technical Summary

Problems solved by technology

However, the stability coefficient of engineering bacteria is low, and there may be hidden safety hazards, and there are still many problems to be solved before industrial production

At present, the main strain of industrial production of lycopene is Blakeslea trispora, but most of the products are mixed carotenoids, and the operation process is complicated.

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