Technology for preparing astaxanthin using microwave-assisted dimethyl sulfoxide method

A technology of dimethyl sulfoxide method and dimethyl sulfoxide, which is applied in the field of biotechnology food additive production, can solve the problem of low wall-breaking effect, and achieve the effects of saving extraction time, reducing energy consumption, and being easy to obtain

Inactive Publication Date: 2009-12-23
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, the research on the extraction of astaxanthin by breaking the wall mainly includes the use of ultrasonic method, acid heat method, dimethyl sulfoxide method, alkali method to break the wall and then extract astaxanthin by organic ...

Method used

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Embodiment Construction

[0015] The present invention is realized through the following steps:

[0016] (1) Obtaining Phaffia rhodozyma fermentation broth and preparation of cells

[0017] a. Seed culture: Take a ring of Phaffia rhodozyma slant seed and insert it into 50mL liquid seed medium (glucose 20g / L, yeast powder 3g / L, peptone 5g / L, 10°Brix wort 3g / L, pH 5.0) in a 250mL Erlenmeyer flask, 20°C, 160r / min shaking culture for 48h, to obtain seed liquid;

[0018] b. Shake flask fermentation culture: Pipette 5 mL of the above-mentioned seed solution into a 500 mL Erlenmeyer flask equipped with 50 mL of shake flask medium (glucose 20 g / L, yeast powder 3 g / L, peptone 5 g / L, pH 5.0), 20 °C, shake culture at 160r / min for 96h to obtain Phaffia rhodozyma fermentation broth;

[0019] c. Cell preparation: Take 10 mL of the fermented Phaffia rhodozyme broth in a centrifuge tube, centrifuge to remove the cells, and wash the cells twice with sterile water to obtain wet cells.

[0020] (2) Microwave-assisted ...

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Abstract

The invention provides a technology for preparing astaxanthin using a microwave-assisted dimethyl sulfoxide method, which comprises the following steps: adding dimethyl sulfoxide after phaffia rhodozyma yeast fermentation liquor is centrifuged; placing the mixture in microwave to assist to crack walls, adding an organic solvent, keeping temperature and lixiviating, turning the astaxanthin from the inside of cells to the outside of the cells and dissolving the astaxanthin into the organic solvent, and centrifuging the mixed solution to undergo low temperature vacuum concentration to obtain an astaxanthin concentrated solution. The invention successfully combines the microwave wall-cracking technology with dimethyl sulfoxide wall-cracking, thereby reducing the cost, improving the efficiency, saving the extraction time, effectively preventing the astaxanthin structure from destroying and improving the extraction effect of the astaxanthin in the phaffia rhodozyma yeast.

Description

(1) Technical field [0001] The invention relates to a technology for extracting astaxanthin from Phaffia rhodozyma, in particular to a microwave-assisted dimethyl sulfoxide method for wall-breaking treatment, and a preparation technology for extracting astaxanthin with an organic solvent, which belongs to the production of biotechnology food additives field. (2) Technical background [0002] Astaxanthin is a pigment widely present in salmon, salmon, shrimp and crab and other crustaceans. Studies have shown that astaxanthin has many important physiological and biological functions such as anti-oxidation, anti-tumor and enhancing immunity. It has broad application prospects in food additives, aquaculture, cosmetics, health products and pharmaceutical industries. [0003] Phaffia rhodozyme is currently a fungus mainly used to produce astaxanthin. Because the cell wall of Phaffia rhodozyme is extremely hard, the wall breaking of Phaffia rhodozyme has a great impact on the extra...

Claims

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Application Information

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IPC IPC(8): C07C403/24B01J19/12
Inventor 朱传合韩伟刘传福
Owner SHANDONG AGRICULTURAL UNIVERSITY
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