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Colloidal gold immune chromatographic test paper box of silver staining enhancement technology and preparation method thereof

An immunochromatographic test paper and immunochromatography technology, which is applied in analytical materials, instruments, measuring devices, etc., to achieve the effects of rapid detection, enhanced detection sensitivity, and easy judgment and storage.

Inactive Publication Date: 2011-07-27
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] After searching, there is no published literature report that this silver staining enhancement technology is applied to colloidal gold immunochromatography test strips to enhance its detection performance

Method used

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  • Colloidal gold immune chromatographic test paper box of silver staining enhancement technology and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of colloidal gold probes

[0027] 1. Preparation of colloidal gold particles

[0028] Take a siliconized 250mL triangular flask, add 100mL deionized water and 1mL 1% chloroauric acid, heat to boil; add 1.5mL1% trisodium citrate accurately while stirring, continue to boil for 15min, and return to the original state with deionized water after cooling. Colloidal gold particles with a particle size of 25 nm can be obtained. Colloidal gold particles with a particle size of 15-40 nm can be prepared by adjusting the amount of 1-2 mL of 1% trisodium citrate added.

[0029] 2. Preparation of colloidal gold-labeled mouse anti-abbrin toxin monoclonal antibody

[0030] Take a siliconized 250mL Erlenmeyer flask, add 100mL of colloidal gold with a particle size of 25 nm or 30nm; add an appropriate amount of 0.2mol / L K 2 CO 3 Adjust the pH to 8.0-9.0, most preferably 8.7, add monoclonal antibody dropwise until the final antibody concentration is 1-1.5mg / mL, most pref...

Embodiment 2

[0065] Ricin immunochromatographic test strips

[0066] Ricin immunochromatography test strip, in which colloidal gold pad is coated with colloidal gold-labeled mouse anti-ricin monoclonal antibody, detection line on nitrocellulose membrane is coated with rabbit anti-ricin polyclonal antibody, quality control line Coated with goat anti-mouse IgG.

[0067] Determination and practice of performance of ricin immunochromatographic test paper

[0068] (1) Sample processing

[0069] Detection of liquid milk-like products: depending on the concentration and viscosity of the sample, make appropriate dilution with 0.01mol / L PBS (pH7.5) solution, and the obtained solution can be used as the detection solution.

[0070] Detection of blood samples: Add 1 volume of 0.01mol / L PBS (pH7.5) solution to 1 volume of blood sample, mix well and let stand for 10 minutes, centrifuge and take the supernatant as the detection solution.

[0071] Detection of food samples: crush the food, add an ...

Embodiment 3

[0077] Canine distemper virus immunochromatographic test strips

[0078] Canine distemper immunochromatographic test strips, in which the colloidal gold pad is coated with colloidal gold-labeled mouse anti-canine distemper virus monoclonal antibody, and the detection line on the nitrocellulose membrane is coated with rabbit anti-canine distemper virus polyclonal antibody. The control line was coated with goat anti-mouse IgG.

[0079] Determination and Practice of Canine Distemper Virus Immunochromatographic Test Paper Performance

[0080] 1. Sample testing

[0081] Take a drop of the sample to be tested and drop it on the sample loading area of ​​the test strip. The sample begins to diffuse on the nitrocellulose membrane. After the gold standard pad is completely released, clear T lines and C lines appear on the nitrocellulose membrane. This reaction It takes about 5-10 minutes. After the reaction is over, place the bottom membrane on the nitrocellulose membrane with tw...

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Abstract

The invention relates to a colloidal gold immune chromatographic test paper box of a silver staining enhancement technology. The test paper box is characterized by mainly comprising a colloidal gold immune chromatographic test paper strip, a silver salt-coated bottom membrane and a top membrane which contains a reducing agent for silver salt reduction reaction. The test paper box has the advantages of easiness for operation, judgment and storage, high sensitivity, high stability, quick detection, clear result, no need of any instrument or equipment and the like, is particularly suitable for field test of clinical and sudden events and is suitable for large-scale application of epidemiological survey.

Description

technical field [0001] The invention provides a colloidal gold immune chromatography test paper box with silver staining enhancement technology, which is used to improve the detection performance of the colloidal gold immune chromatography test paper strip. The application in detection belongs to the field of biological detection. Background technique [0002] Colloidal gold probe is a new technology developed in the 1970s. What is particularly eye-catching is the rapid diagnosis technology of colloidal gold with membrane as the solid phase carrier. A new type of in vitro diagnostic technology developed on the basis of In recent years, this technology has been paid more and more attention by people, and its technology has developed rapidly, and it has been widely used in the field of biomedicine, especially in human medicine and veterinary medicine. [0003] Colloidal gold immunochromatographic test strips are made by combining the reaction specificity of immunocolloidal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/566G01N33/558G01N33/532
Inventor 李小兵刘国文杨威张燚孔涛张志刚张冰冰唐佳佳李冬娜王哲
Owner JILIN UNIV
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