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Method for showing fingerprints on various object surfaces and keeping DNA information

A fingerprint and object technology, applied in the field of detection, achieves the effects of high sensitivity, easy preparation and storage, and low cost

Inactive Publication Date: 2010-05-12
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When tetramethylbenzidine shows blood fingerprints, DNA detection will not be affected when DNA detection is performed, but there is a certain requirement for blood volume, and occult blood fingerprints with less blood often affect the detection of DNA information

Method used

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  • Method for showing fingerprints on various object surfaces and keeping DNA information
  • Method for showing fingerprints on various object surfaces and keeping DNA information
  • Method for showing fingerprints on various object surfaces and keeping DNA information

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Tryptophan functionalized gold nanoparticles

[0039] Preparation: Mix and stir the chloroauric acid aqueous solution and the tryptophan aqueous solution, the ratio of substances is 9:10, put half of the mixed solution into a polytetrafluoroethylene reactor, put it in an oven and heat it to 100°C, and continue to react for 0.5 hours. After natural cooling, gold nanoparticles functionalized with tryptophan were obtained, with a particle size of 25-30 nm.

[0040] Visualization: Immerse the A4 paper, glass sheet, copper sheet and other samples with latent fingerprints in the above gold nanoparticle solution for 10 minutes, then take out the sample and rinse it with tap water to wash off the gold nanoparticles that have not reacted with the components in the fingerprint, and then immerse in the gold nanoparticle solution. In the prepared silver staining solution, soak the sample for 5 minutes, take it out, rinse it with tap water and dry it to get a clear finger...

Embodiment 2

[0041] Example 2: Tryptophan functionalized gold nanoparticles

[0042] The chloroauric acid aqueous solution and the tryptophan aqueous solution are mixed, the substance ratio is 9:10, and slowly heated to 50°C to obtain tryptophan-functionalized gold nanoparticles with a particle size of 25-30nm.

[0043] Visualization: Immerse the A4 paper, glass sheet, copper sheet and other samples with latent fingerprints in the above gold nanoparticle solution for 5 minutes, then take out the sample and rinse it with tap water to wash off the gold nanoparticles that have not reacted with the components in the fingerprint, and then immerse in the gold nanoparticle solution. In the prepared silver staining solution, soak the sample for 10 minutes, take it out, rinse it with tap water and dry it to get a clear fingerprint image. (attached figure 2 )

Embodiment 3

[0044] Example 3: Gold nanoparticles functionalized with glutathione

[0045]Preparation: At room temperature, mix and stir 2.5mM chloroauric acid aqueous solution and 1.9mM glutathione aqueous solution, the volume ratio is 1:7.8, adjust the pH of the solution to 8 with 1M / L sodium hydroxide, add the newly prepared hydroboration The sodium ice solution was reduced, and the stirring was continued for 12 hours to obtain glutathione functionalized gold nanoparticles.

[0046] Visualization: Immerse the A4 paper, glass sheet, copper sheet and other samples with latent fingerprints in the above gold nanoparticle solution for 10 minutes, then take out the sample and rinse it with tap water to wash off the gold nanoparticles that have not reacted with the components in the fingerprint, and then immerse in the gold nanoparticle solution. In the prepared silver staining solution, soak the sample for 10 minutes, take it out, rinse it with tap water and dry it to get a clear fingerprint ...

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Abstract

The invention belongs to the technical field of detection, in particular to a method for showing fingerprints on various object surfaces and keeping DNA information. The method adopts metaliding, takes functionalized gold nanoparticles and silver nanoparticles as probes, and is combined with argentation to realize that the latent fingerprints and blood fingerprints on different object surfaces can be shown in one method. The gold and silver nanoparticles are adsorbed due to the action with the ingredients of the latent and blood fingerprints, silver ions in silver staining solution can be catalyzed to be reduced on the surfaces of the fingerprints, and the generated silver covers the surfaces of the gold nanoparticles, thus enhancing the detection signals. After the blood fingerprints are shown in the method, the DNA sequence can be further detected. In the invention, the reducing agent in the silver staining solution is further expanded, when vitamin C is taken as the reducing agent, the cost is lower, the reaction condition is wild, the operation is simpler, and further environmental protection can be realized. The method has high sensitivity and wide application range, and can show old fingerprints.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a latent sweat and blood fingerprint display method suitable for retaining DNA information on the surface of various objects. The DNA sequence detection can still be carried out after the fingerprint is displayed by the method. Background technique [0002] Fingerprints play an important evidential role in forensic science and criminal proceedings. More than 5 billion people in the world have their own fingerprints. Even if the skin of the fingers peels off, the new skin grows back, and the pattern remains the same. Therefore, fingerprinting has become a traditional method of criminal investigation. However, in all kinds of crimes, criminals use more and more insidious and hidden means of committing crimes. Fingerprints that appear at crime scenes are often latent and require some visualization method to observe their texture. Therefore, scientifically and corre...

Claims

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Application Information

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IPC IPC(8): A61B5/117A61B5/1172
Inventor 柴芳苏忠民王春刚马占芳王婷婷
Owner NORTHEAST NORMAL UNIVERSITY
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