Method used for identifying different families of procambarus clarkii

A technology of Procambarus clarkii and family line, which is applied in the field of molecular markers, can solve the problems of the quality degradation of Procambarus clarkii, affect the growth of Procambarus clarkii, cost money and labor, and achieve the effect of preventing inbreeding and selective breeding

Inactive Publication Date: 2016-05-25
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, there is no effective method to identify the parent-child relationship of Crayfish
The traditional parent-child relationship identification method is to raise the families separately or mark the eyestalks. These two methods are not only costly and laborious, but also marking the eyestalks will directly affect the growth of Crayfish.
Just because there is no quick and effective method to identify the parent-

Method used

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  • Method used for identifying different families of procambarus clarkii
  • Method used for identifying different families of procambarus clarkii
  • Method used for identifying different families of procambarus clarkii

Examples

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Embodiment 1

[0022] (1) Breeding of Procambarus clarkii

[0023] Three female shrimps with eggs were randomly selected from the aquatic product market in Wuxi City, Jiangsu Province, respectively No. 1 shrimp, No. 2 shrimp, and No. 14 larvae were taken from the offspring of No. 3 shrimp, and 15 larvae were taken from the offspring of No. 3 shrimp to form three half-sib families, with a total of 46 individuals, 1 being No. 1 shrimp, and 2 to 15 being the offspring of No. 1 shrimp , 16 is No. 2 shrimp, 17 to 30 are the offspring of No. 2 shrimp, 31 are No. 3 shrimp, and 32 to 46 are the offspring of No. 3 shrimp.

[0024] (2) Extraction of DNA of Procambarus clarkii parents and larvae:

[0025] 1. Take 0.2g muscle from the tail muscle of Procambarus prawns, put it into a 1.5ml centrifuge tube, and add 470ulSET. Muscles are crushed with a grinding stick.

[0026] 2. Add 25ul of 20% SDS (final concentration is 0.5%), and then add 5ul of 20mg / ml proteinase K (final concentration of 200ug / ml)...

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Abstract

The invention discloses a method used for identifying different families of procambarus clarkii. The method comprises following steps: half-sib families are established artificially; DNA is extracted from the tail muscle of procambarus clarkii; 10 pairs of selected microsatellite primers are used for PCR amplification of the extracted DNA; obtained PCR products are subjected to electrophoretic analysis and silver staining; silver staining results are subjected to digital analysis; genetic distances of individuals are measured using a genetic software, a UPGMA cluster analysis diagram is drawn based on the genetic distances, and the individuals from different families can be distinguished based on cluster analysis results. The method can be used for distinguishing the families of procambarus clarkii quickly and accurately, and preventing inbreeding effectively, and possesses high application value in breeding of procambarus clarkii families.

Description

technical field [0001] The invention relates to a method for identifying different families of Procambarus clarkii, belonging to the technical field of molecular markers. Background technique [0002] Procambarus clarkii (Procambarus clarkii), commonly known as crayfish in the folk, also known as freshwater crayfish or red crayfish, belongs to the decapod order, crayfish family, and the genus Procambarus clarkii. It is a lobster-like back that lives in fresh water. Animals with hard shells are very tenacious in survival. They are native to South America and northern Mexico. Now Procambarus clarkii has become an important freshwater economically farmed animal in my country. It is mainly distributed in small rivers, lakes and small ditches in the wild. [0003] Paternity testing technology refers to the use of principles and technologies in medicine or in production to distinguish whether there are disputes between father and son and the judgment of the distance of kinship. Pa...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 沈怀舜胡亚成马源潮
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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