Method for marking non-heading cabbage molecule by utilizing SAMPL technique

A technology of head cabbage and molecular markers, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc.
CN101260433AInactive Publication Date: 2008-09-10SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2008-09-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for loose head cabbage molecular markers by utilizing the SAMPL technology comprising DNA extraction, DNA enzyme digestion-connection system optimization, SAMPL pre-amplification system optimization, SAMPL amplification system optimization, electrophoresis, silver staining color development and cluster analysis, wherein, a DNA sample is performed enzyme digestion through using EcoRI, MseI or PstI enzyme, and then corresponding joints of EcoRI,MseI or PstI are connected on the DNA sample through using T4-DAN ligase; the SAMPL pre-amplification is performed through using a 10mu L reaction system; a pre-amplification primer is corresponding to the chosen enzyme in the enzyme digestion; the SAMPL amplification is performed through using a 20mu L reaction system, one amplification primer is corresponding to the pre-amplification primer, and the other is an SAMPL primer. The method for loose head cabbage molecular markers by utilizing the SAMPL technology performs optimization and primer design for the complicated system of the SAMPL, constructs an SAMPL reaction system applicable to loose head cabbages and realizes loose head cabbage molecular markers.
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Description

technical field

[0001] The invention relates to a molecular marker method in the technical field of crop breeding, in particular to a method for molecular marker of non-heading Chinese cabbage by using SAMPL (selectively amplified polymorphic microsatellite site) technology. Background technique

[0002] Second-generation molecular markers based on PCR (polymerase chain reaction) technology, such as AFLP (amplified fragment length polymorphism) and SSR (simple sequence repeat), have become important technical means of molecular marker-assisted selection. As the combination of modern molecular biology and traditional genetic breeding, molecular marker-assisted selection technology can select breeding materials at the DNA level with the help of molecular markers, speed up the selection process, and enhance the accuracy of selection, thereby significantly improving breeding efficiency. So the second generation molecular marker technology has been widely used in breeding work. ...

Claims

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