Method for quantitative detection of mercury ions through gold label silver stain and kit thereof

A technology of gold standard silver staining and quantitative detection, which is applied in the preparation of test samples, material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc., to achieve high sensitivity, low price, Ease of pervasive effect
CN102759526AInactive Publication Date: 2012-10-31NINGBO UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NINGBO UNIV
Publication Date
2012-10-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for the quantitative detection of mercury ions through gold label silver stain and a kit thereof. The method is characterized by comprising the following steps of: heating, washing and drying a glass slide, immersing the glass slide in an APTES (3-Aminopropyltriethoxysilane) solution, performing silanization treatment on the glass slide at room temperature, then cleaning the glass slide with ethanol, and drying in vacuum at 110-130 DEG C for 1-3h to obtain the silanization glass slide; mixing DNA and a nanogold solution, vibrating to reach a uniform state, incubating at room temperature, and adding water for dilution to obtain a specific DNA-nanogold probe solution; mixing the specific DNA-nanogold probe solution with a sample solution, dripping the mixture on the silanization glass slide, drying a sample point at room temperature completely, dripping a silver staining reagent, standing in a black box, then, immediately rinsing the glass slide with distilled water and drying; and finally, performing quantitative analysis on gold label silver stain signals, and calculating to obtain the accurate concentration of the mercury ions in the sample solution to be detected. The method has the advantages of high sensitivity, high selectivity, availability for quantitative analysis of mercury ion concentration, simplicity for operation and availability for field detection.
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Description

technical field

[0001] The invention relates to a method for detecting mercury ions, in particular to a method for quantitatively detecting mercury ions by gold-labeled silver staining and a kit thereof. Background technique

[0002] Mercury (Hg), the only liquid metal at room temperature, is one of the heavy metals. In recent years, the mining, smelting, processing and commercial manufacturing activities of heavy metal mercury have been increasing, and a large amount of mercury has entered the atmosphere, water and soil for retention, accumulation and migration. Mercury is highly toxic and remains in the organism and the environment for a long time, which seriously threatens human health. Therefore, many countries and regions such as the United States, the European Union, Canada, and my country have strictly limited the mercury content in food, daily necessities, and the environment. Mercury Pollution detection and monitoring has become a major concern. Mercury in nature i...

Claims

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