Fast typing detection method of high-flux visualized HPV (Human Papilloma Virus) gene chip

A technology of human papillomavirus and gene chip, which is applied in the field of economical diagnosis, can solve the problems of high detection cost, the detection result cannot be directly seen by the naked eye, and is expensive, and achieves the effects of high sensitivity, stable detection result and easy operation

Active Publication Date: 2010-08-04
SHANXI LIFEGEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

In this new technology, there's an improved way to diagnose viral disease through immunological methods like histology or fluorescence analysis. These techniques have been developed over several decades ago but they still require costly specialized instruments such as microscopes or labs due to their complexity. However these tests provide quicker and more accurate data compared to traditional laboratory testing procedures. They may help identify individuals who harbor latently infected cells from others during routine vaginal smears while avoiding invasive tissue biopsy procedures. Overall, it provides technical benefits that make them easier than current technologies at home and work.

Problems solved by technology

This patented technical problem addressed in this patents relates to developing methods that could accurately diagnose or predict outbreaks caused by Human Papillovirus (Human Papiliary Virus) virus infections without requiring invasive procedures like tissue histo culture. Current techniques involve expensive equipment and require complicated analysis processes which may result in errors during interpretation.

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  • Fast typing detection method of high-flux visualized HPV (Human Papilloma Virus) gene chip
  • Fast typing detection method of high-flux visualized HPV (Human Papilloma Virus) gene chip
  • Fast typing detection method of high-flux visualized HPV (Human Papilloma Virus) gene chip

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Embodiment Construction

[0073] The high-throughput visual human papillomavirus gene chip rapid typing detection method adopted by the present invention has the following steps:

[0074] Known techniques can be used for detection probes and chip spotting in the prepared chip of the present invention.

[0075] (1) Preparation of chips

[0076] (1.1) Design detection probe

[0077] Design detection probes according to 13 subtypes of human papillomavirus, the probe sequences are as follows:

[0078] HPV 6 ATCCGTAACTACATCTTCCACATACACCAA

[0079] HPV 11 ATCTGTGTCTAAATCTGCTACATACACTAA

[0080] HPV 16 GTCATTATGTGCTGCCATATCTACTTCAGA

[0081] HPV 18 TGCTTCTACACAGTCTCCTGTACCTGGGCA

[0082] HPV 31 TGTTTGTGCTGCAATTGCAAACAGTGATAC

[0083] HPV 33 TTTATGCACACAAGTAACTAGTGACAGTAC

[0084] HPV 35 GTCTGTGTGTTCTGCTGTGTCTTCTAGTGA

[0085] HPV 45 ACACAAAATCCTGTGCCAAGTACAT

[0086] HPV 51 AGCACTGCCACTGCTGCGGTTTCCCCAACA

[0087] HPV 52 TGCTGAGGTTAAAAAGGAAAGCACATATAA

[0088] HPV 53 TCTATGTCTACATATAATTCAAAAGCAAAT ...

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Abstract

The invention relates to a fast typing detection method of a high-flux visualized HPV (Human Papilloma Virus). In the method, a DNA probe is marked by using colloidal gold, and a gold signal is improved by silver dying and judged directly and visually without expensive signal detection equipment. The invention has simple and convenient operation, time saving, high sensitivity, stable detection result and good accuracy and solves the defect that a traditional detection result can not be directly viewed by naked eyes but needs a corresponding instrument for detection. A gene chip integration system for HPV typing detection can rapidly investigate infected HPV hypotypes, and detection objects can cover a plurality of domestic common high-incidence and serious-damage HPV hypotypes. By the chip design, a plurality of high-flux clinical detection requirements of a plurality of patients can be met through one examination. The method can be widely used for clinical diagnosis and female physical examination or the screening of early diseases, such as cervical cancer, condyloma, and the like and has important meaning to the clinical diagnosis of HPV and the early prevention of cervical cancers.

Description

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Claims

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Application Information

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Owner SHANXI LIFEGEN
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