Fast typing detection method of high-flux visualized HPV (Human Papilloma Virus) gene chip

Abstract

The invention relates to a fast typing detection method of a high-flux visualized HPV (Human Papilloma Virus). In the method, a DNA probe is marked by using colloidal gold, and a gold signal is improved by silver dying and judged directly and visually without expensive signal detection equipment. The invention has simple and convenient operation, time saving, high sensitivity, stable detection result and good accuracy and solves the defect that a traditional detection result can not be directly viewed by naked eyes but needs a corresponding instrument for detection. A gene chip integration system for HPV typing detection can rapidly investigate infected HPV hypotypes, and detection objects can cover a plurality of domestic common high-incidence and serious-damage HPV hypotypes. By the chip design, a plurality of high-flux clinical detection requirements of a plurality of patients can be met through one examination. The method can be widely used for clinical diagnosis and female physical examination or the screening of early diseases, such as cervical cancer, condyloma, and the like and has important meaning to the clinical diagnosis of HPV and the early prevention of cervical cancers.

Description

technical field [0001] The invention belongs to the field related to gene detection, and relates to a high-throughput visual method for rapidly typing and detecting human papillomavirus (HPV). Background technique [0002] Cervical cancer is a common malignant tumor in gynecology, and its mortality rate is second only to breast cancer, ranking second in cancer deaths among women. There are about 131,500 new cases of cervical cancer in my country every year, accounting for 28.8% of the total number of new cases of cervical cancer in the world. In addition, there are far more diseases missed due to conditional restrictions. Therefore, early detection and treatment is the key to improving the survival rate of cervical cancer patients. At present, HPV cannot be cultured in vitro. Most patients with HPV infection have no basis for microscopic diagnosis. In addition, serological tests are not sensitive, and most of them are negative. Therefore, the detection of HPV infection ma...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a high-throughput visual human papillomavirus (HPV) gene chip rapid typing detection method, which solves the problem of tracing in the detection method of the human papillomavirus gene chip in the background technology. Most of the needles are enzymes, fluorescent, radioactive isotopes, chemiluminescent substances, etc. The test results cannot be directly seen by the naked eye, and need to be tested by expensive instruments, which is a technical problem with high testing costs

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