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Colloidal gold method detection test strip and reagent kit for IgG antibody of respiratory disease and preparation method of reagent kit

A technology for detecting test strips and respiratory tracts, which is applied in the field of immunodiagnostic reagents, can solve the problems of insufficient convenient and fast detection, cumbersome operation process, etc., and achieves the effects of simple detection, simplified operation process, and easy popularization.

Inactive Publication Date: 2013-02-13
JIANGSU KEYGEN BIOTECH CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the detection of pathogenic IgG antibodies alone, comprehensive analysis of the test results has no effect on the diagnosis of the disease, but the operation process is cumbersome and the detection is not convenient and fast enough.

Method used

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  • Colloidal gold method detection test strip and reagent kit for IgG antibody of respiratory disease and preparation method of reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Composition of Respiratory Disease IgG Antibody Combined Detection Kit (Colloidal Gold Method)

[0027] 1. Sample processing bottle: The sample processing bottle (purchased from Hangzhou Beiyu) is filled with 1mL sample processing solution (phosphate buffer solution, the main raw material is purchased from Sinopharm Group).

[0028] 2. Test strip: 60*5mm bottom plate (purchased from Shanghai Jieyi Company); the absorbent pad is 15*5mm coarse fiber filter paper (purchased from Shanghai Jieyi Company); 25*5mm nitrocellulose membrane (purchased from Mill ipore company) were coated sequentially with Respiratory Herpes Virus Recombinant Antigen (purchased from Meridian Company), Adenovirus Recombinant Antigen (purchased from Meridian Company), Influenza Virus Recombinant Antigen (purchased from Meridian Company), Mycoplasma pneumoniae Recombinant Antigen (purchased from Meridian Company) company), goat anti-rabbit antibody (purchased from abcam company); the sampl...

Embodiment 2

[0029] Embodiment 2: the preparation of kit

[0030] 1. Preparation of sample processing solution: Prepare sample processing solution: prepare phosphate buffer solution (pH7.0±0.2), dispense it into sample processing bottles, 1mL / bottle.

[0031] 2. Preparation of test strips:

[0032] 2.1. Dilute Respiratory Herpes Virus Recombinant Antigen, Adenovirus Recombinant Antigen, Influenza Recombinant Antigen, Mycoplasma pneumoniae Recombinant Antigen, Goat Anti-Rabbit Antibody to a coating concentration of 0.03-0.05mg / mL, and the five coating solutions were respectively Scratch to the designated position on the nitrocellulose membrane, dry the coated nitrocellulose membrane and store it at 37°C for 4 hours.

[0033] 2.2. Preparation of gold standard pad: Colloidal gold was prepared by trisodium citrate reduction method, and rabbit anti-human antibody was slowly added to the gold sol at a ratio of 20 μg / mL to 40 μg / mL. After fully reacting, centrifuge at 8000 RPM for 30 minutes, di...

Embodiment 3

[0038] Embodiment 3 detection method

[0039] 1. Test samples according to clinical routines. The test samples can be serum, whole blood, sputum, and throat swabs. If the sample cannot be detected within three days, it can be stored at 2-8°C, and the storage period should not exceed 7 days.

[0040] 2. Take the sputum and throat test into the sample processing bottle, shake the specimen well, and let it stand for 5 minutes. Serum and whole blood samples do not require this step.

[0041] 3. Take 100-200 ul of the processed sample or serum or whole blood sample and add dropwise to the sample loading window. If the whole blood is relatively viscous, add 1 to 2 drops of unused sample processing solution to the sample loading window.

[0042] 4. Interpret the result after 10-15 minutes:

[0043] 4.1. If the sample contains respiratory herpes virus IgG antibody, adenovirus IgG antibody, influenza virus IgG antibody, and mycoplasma pneumoniae IgG antibody, it will bind to the rab...

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Abstract

The invention discloses a colloidal gold method detection test strip and a reagent kit for an IgG antibody of a respiratory disease and a preparation method of the reagent kit. The test strip determines the IgG antibody by using a principle of an immunocapture method; respiratory herpes viruses, adenoviruses, influenzaviruses and an IgG antibody of mycoplasma pneumoniae can be detected jointly by one operation; the operation process is simplified; the test strip is simple, convenient, rapid and accurate in detection, suitable for mass detection and applicable to primary screening and epidemiological survey; and a result is distinct and easy to distinguish.

Description

technical field [0001] The invention belongs to the technical field of immunodiagnostic reagents, and relates to a colloidal gold test strip, kit and preparation method for detecting IgG antibodies of respiratory diseases, in particular to a four-in-one test strip that can detect four indicators of common respiratory virus infection at the same time. IgG antibody-linked colloidal gold rapid detection test strip, kit and preparation method thereof. Background technique [0002] Immunoglobulin G (Immunoglobulin G, IgG) is one of the most important antibodies in humans or animals. After an antigen (bacteria or virus, etc.) enters the body, the first immunoglobulin that appears is IgM antibody, and then IgG antibody appears, but the half-life of IgM in the human body is very short, which is often not conducive to detection and diagnosis. Although IgA antibody may also appear in some cases, because its clinical significance is basically the same as that of IgG, and the IgA conte...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/531
Inventor 不公告发明人
Owner JIANGSU KEYGEN BIOTECH CORP LTD
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