Fluorescent quantitation PCR (polymerase chain reaction) detection kit for four conventional herpesvirus hominises

Abstract

The invention provides a real-time fluorescent quantitation PCR (polymerase chain reaction) kit for simultaneously detecting conventional herpesvirus hominises HSV (herpes simplex virus)-1, HSV-2, EBV and HCMV (human cytomegalovinis). The kit consists of quantitation PCR reaction liquid, quantitation reaction liquid, an HSV-1 standard substance, an HSV-2 standard substance, an EBV standard substance, an HCMV standard substance, a negative reference substance, an HSV-1 positive reference substance, an HSV-2 positive reference substance, an EBV positive reference substance, an HCMV positive reference substance, a specification and a kit body. The kit disclosed by the invention adopts a real-time fluorescent quantitation PCR technology and two specific fluorescent probes in the same reaction system; the detection range covers the four most conventional herpesvirus hominises, so that the four herpesvirus hominises in a sample can be simultaneously subjected to parting detection; real-time and accurate quantitation can be realized; the need for early, quick and accurate diagnosis can be met; a powerful basis is supplied to epidemiological investigation and immediate formulation of a targeted treating scheme.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a fluorescent quantitative PCR detection kit, in particular to a real-time fluorescent quantitative PCR detection of herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 in samples such as blood, urine, and cerebrospinal fluid of patients (HSV-2), Epstein-Marr virus (EBV), and human cytomegalovirus (HCMV) are novel kits for four common human herpesviruses. Background technique [0002] Herpesviridae is a group of medium-sized, enveloped DNA viruses, more than 100 species have been discovered. Herpesviruses known to be associated with human disease include herpes simplex virus types 1 and 2 (HSV-1, HSV-2), varicella-zoster virus (VZV), Epstein-Marr virus (EBV), human giant Cytovirus (HCMV), human herpesvirus types 6, 7 and 8 (HHV-6, HHV-7, HHV-8), the first four viruses are the most common clinically. When the body's immune function is low or suppressed, it is e...

AI Technical Summary

Problems solved by technology

[0003] Traditional herpes virus detection methods mainly include virus isolation and culture and serological detection. The former was once the "gold standard" for the diagnosis of herpes virus, but it takes a long time (3-30 days) and has low sensitivity (especially after antiviral drug treatment). ), typing difficulties and other defects; the latter is divided into direct antigen detection method and antibody indirect detection method. The direct antigen detection method has low sensitivity because some viruses do not produce detectable antigens, and the indirect antibody detection method is due to herpes virus It takes about 1 week after infection to produce an effective concentration of antibodies, making early diagnosis impossible, and there are cross-reactions between different herpes viruses, and the specificity of antibodies is not high

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