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Complex microbial inoculant capable of promoting growth of larva of Hermetia illucens and application thereof

A technology for the bright-spotted water flies and compound bacterial agent, which can be applied in the fields of agricultural microbiology, ecological agriculture, and environmental protection, can solve problems such as environmental pollution, achieve significant economic value, and improve economic benefits.

Inactive Publication Date: 2017-06-16
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Another object of the present invention is to provide an application of a composite bacterial agent for promoting the growth of soldier fly larvae, including promoting the growth of soldier fly larvae and promoting the transformation of chicken manure. The bacterial agent provided by the invention It can fully solve the environmental pollution problem caused by the accumulation of feces in the current intensive farms, and at the same time turn waste into treasure and transform it into a high-quality animal protein substitute

Method used

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  • Complex microbial inoculant capable of promoting growth of larva of Hermetia illucens and application thereof
  • Complex microbial inoculant capable of promoting growth of larva of Hermetia illucens and application thereof
  • Complex microbial inoculant capable of promoting growth of larva of Hermetia illucens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Strain acquisition:

[0033] The applicant isolated four strains of bacteria from the surface and intestine of soldier fly eggs. The preservation information and physiological and biochemical characteristics of the four strains are as follows:

[0034] Kocuria marina (or FE01 in the present invention): classification name: Kocuria marina FE01, preservation number: CCTCC NO: M 2015558; it was sent to the China Center for Type Culture Collection on September 18, 2015 Preservation, address: Wuhan University, Wuhan, China. Physiological and biochemical characteristics: On LB medium, the colony is yellow, and the shape of the colony is neat, raised, moist, without wrinkles, and medium in size. Gram-positive cocci, cultivated at 28°C, aerobic bacteria. It has protease (Up=1.60), cellulase (Up=2.00) and amylase (Up=2.50) hydrolytic activities.

[0035] Boron-resistant lysine bacillus (or FE04 in the present invention): taxonomic name: Lysinibacillus boronitolerans FE04, pre...

Embodiment 2

[0043] Fermentation methods of FE01, FE04, FE08 and BSF-CL single strains:

[0044] FE01: LB liquid medium, the inoculum size is 1%, 180r / min, 28°C shake flask fermentation for 24h.

[0045] FE04: LB liquid medium, the inoculum size is 1%, 180r / min, 28°C shake flask fermentation for 24h.

[0046] FE08: LB liquid medium, inoculum size 1%, 180r / min, 28°C shake flask fermentation for 24h.

[0047] BSF-CL: LB liquid medium, the inoculum size is 1%, 180r / min, 28°C shake flask fermentation for 24h.

[0048] Example 2:

[0049] Application of single strain fermentation broth of FE01, FE04, FE08 and BSF-CL:

[0050] Insert 10ml of LB liquid medium into the PA bottle. Single colonies of FE01, FE04, FE08 and BSF-CL were picked with a sterile inoculation loop, inoculated into PA bottles, and fermented in shake flasks at 28°C for 24 hours. Use sterile water to adjust the concentration of the fermentation broth to 1 × 10 8 per ml, mix the diluted bacterial solution with chicken manur...

Embodiment 3

[0074] A compound microbial agent for promoting the growth of soldier fly larvae, including FE01, FE04, FE08 and BSF-CL.

[0075] The present embodiment has prepared 9 groups of composite bacterial agents, and the specific formula is as follows (V / V):

[0076] Adjust the concentration of FE01, FE04, FE08 and BSF-CL fermentation broth to 1×10 8 pcs / ml, set 9 compounding combinations, the compounding ratio is as follows:

[0077] Group 1: FE01: FE04: FE08: BSF-CL = 1:1:1:1

[0078] Group 2: FE01: FE04: FE08: BSF-CL = 2:1:1:1

[0079] Group 3: FE01: FE04: FE08: BSF-CL = 4:1:1:1

[0080] Group 4: FE01: FE04: FE08: BSF-CL = 1:2:1:1

[0081] Group 5: FE01: FE04: FE08: BSF-CL = 1:4:1:1

[0082]Group 6: FE01: FE04: FE08: BSF-CL = 1:1:2:1

[0083] Group 7: FE01: FE04: FE08: BSF-CL = 1:1:4:1

[0084] Group 8: FE01: FE04: FE08: BSF-CL = 1:1:1:2

[0085] Group 9: FE01: FE04: FE08: BSF-CL = 1:1:1:4

[0086] Used in the following examples.

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Abstract

The invention discloses a complex microbial inoculant capable of promoting growth of the larva of Hermetia illucens and application thereof. The complex microbial inoculant in the invention comprises Kocuria marina FE01, Lysinibacillus boronitolerans FE04, Proteus mirabilis FE08 and Bacillus subtilis BSF-CL. The complex microbial inoculant is used for promoting the growth of Hermetia illucens during transformation of chicken manure via Hermetia illucens, and is safe and environment-friendly. As the complex microbial inoculant is added into chicken manure containing Hermetia illucens, the weight gain of Hermetia illucens is increased by 28.57% compared with a chicken manure transformation system without the complex microbial inoculant; and the complex microbial inoculant can substantially improve economic benefits in large-scale chicken manure transformation via Hermetia illucens and has great economic value.

Description

technical field [0001] The invention belongs to the fields of agricultural microbiology, ecological agriculture, environmental protection and the like, and in particular relates to a compound bacterial agent for promoting the growth of soldier fly larvae and its application. Background technique [0002] 1. Light-spotted flat-horned soldier fly [0003] Black soldier fly (Hermetiaillucens L), English name Black soldier fly, is a kind of insect belonging to Diptera, Soldieridae, genus Hermetiaillucens, species of Hermetiaillucens L. Its larvae can reach a length of 20 mm. It originated in North America and has become a worldwide widespread insect. It is distributed between 45° north latitude and 40° south latitude. It is found in America, Europe, Asia, Oceania and many Pacific islands. There are records on it. my country's light-spotted soldier fly is mainly distributed in central and southern China. The growth and development stages of the soldier fly can be divided into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01K67/033C12R1/125C12R1/37C12R1/01
CPCA01K67/033A01K2227/706C12N1/20C12N1/205C12R2001/125
Inventor 张吉斌郑龙玉肖小朋蔡珉敏喻子牛张鼎楠宋旗周定中曹露
Owner HUAZHONG AGRI UNIV
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