Specific nucleic acid identification sequence used for detecting proteus mirabilis, and application thereof

A technology of proteus mirabilis and identification sequence, which is applied in the field of specific nucleic acid identification sequence to achieve the effect of short time, high sensitivity and specificity

Inactive Publication Date: 2012-07-11
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still relatively few specific nucleic acid identification sequences that can be used for PCR detection of Proteus mirabilis. The currently known nucleic acid identification sequences of Proteus mirabilis mainly include 16S sequence, 16S-23S sequence, ureC sequence and a section of unknown function. nucleic acid fragment

Method used

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  • Specific nucleic acid identification sequence used for detecting proteus mirabilis, and application thereof
  • Specific nucleic acid identification sequence used for detecting proteus mirabilis, and application thereof
  • Specific nucleic acid identification sequence used for detecting proteus mirabilis, and application thereof

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Embodiment 1

[0040] 2. Design and verification of the specific nucleic acid identification sequence of Proteus mirabilis

[0041] (1) According to the nucleic acid sequence of Proteus mirabilis ureR, primers ureRF1: 5'-GGTGAGATTTGTATTAATGG-3' and ureRR1: 5'-ATAATCTGGAAGATGACGAG-3' were designed. Use this nucleic acid identification sequence ureRF1 and ureRR1 as the forward and reverse primers of the PCR reaction respectively, and use the isolated Proteus mirabilis V7 (CGMCC No.4312) and Providencia stutzeri containing the ureR homologous gene, Escherichia Bacillus, Escherichia coli, and some common pathogens in the environment (strains include Vibrio, Edwardsiella, Klebsiella, and Pseudomonas strains) as template PCR to determine the use of PCR for the detection of Proteus mirabilis specificity.

[0042] (2) 15 μl of the PCR reaction system were added the following volumes of substances: 1.5 μl of 10×PCR buffer (200 mmol l -1 Tris-HCl (pH 8.4), 200mmol l -1 KCl, 15 mmoll -1 MgCl 2 )...

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Abstract

The invention relates to the detection of proteus mirabilis, and specifically relates to a specific nucleic acid identification sequence used for detecting proteus mirabilis, and an application thereof. The specific nucleic acid identification sequence used for detecting proteus mirabilis is ureRF1:5'-GGTGAGATTTGTATTAATGG-3'; ureRR1:5'-ATAATCTGGAAGATGACGAG-3'. The proteus mirabilis specific nucleic acid identification sequence is used for detecting proteus mirabilis in the environment. When proteus mirabilis in different environments are detected by using a method provided by the invention, advantages such as convenient operation, high speed, high specificity and high sensitivity are provided. The method can be used in proteus mirabilis detection of environments and clinical samples, and epidemiological studies. The sequence and the method have wide application prospect.

Description

technical field [0001] The invention relates to the detection of Proteus mirabilis, in particular to a specific nucleic acid identification sequence for detecting Proteus mirabilis and its application. Background technique [0002] Proteus mirabilis belongs to the genus Proteus and widely exists in polluted environments such as soil, water and feces. Proteus mirabilis is an opportunistic pathogenic bacteria in the environment, and it is a common pathogenic bacteria that can infect humans and cause human diseases. In recent years, the clinical incidence of food poisoning caused by Proteus mirabilis has increased significantly, which seriously threatens people's health. However, the detection method of Proteus mirabilis is still relatively backward, and there are few reports on the research on the detection method of this bacteria at home and abroad. [0003] At present, traditional methods are mostly used for the detection of Proteus mirabilis, mainly based on laboratory se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12R1/37
Inventor 陈令新张卫卫牛宗亮殷堃
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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