Escherichia coli O1, O2, O18 and O78 serotype detection kit and detection method thereof

Abstract

The invention belongs to the technical field of biology detection and relates to an escherichia coli O1, O2, O18 and O78 serotype detection kit and a detection method thereof. Forward primers of a primer group of the kit are highly conservative escherichia coli O antigen combined relative gene sequences, and backward primers are four serotype specific primers designed on the basis of four O1, O2, O18 and O78 serotype escherichia coli O antigen synthesis relative genes. The detection kit containing the primer group and the detection method of the detection kit have the advantages of being fast, sensitive, specific, low in cost, easy to operate and capable of well overcoming the shortcomings in the current escherichia coli O1, O2, O18 and O78 serotype detection method, can meet the requirements for the current escherichia coli O1, O2, O18 and O78 serotype detection, can be popularized and used easily in a wide range and have wide market prospect and large economic benefit.

Description

Technical field [0001] The present invention is a biological test technology field, which involves a type of testing dose and testing methods for O1, O2, O18, O78, and its detection methods. Background technique [0002] E. coli is the common name of Escherichia Coli (E.COLI). It belongs to the Enterobacteriaceae and Escherichia.The infant's feces are separated, which is named after the colon.There are many types of E. coli in nature. Most of them are non -pathogenic E. coli are the main members of normal flora in human and animal intestines, and they have positive significance to maintain the normal physiological function of the intestine.However, some special serum types are pathogenic, which can cause diarrhea, urinary tract infections, pneumonia, sepsis, endometritis, early childitis, poultry's yolk peritonitis, granuloma, etc.Narao J P, MOBLEY H L.Pathogenic Escherichia Coli.nat Rev Microbiol, 2004,2 (2): 123-140). [0003] E. coli antigen is complicated, mainly bacterial an...

AI Technical Summary

Problems solved by technology

However, previous reports all used the single-plex PCR method, which can only detect one serotype at a time, which is time-consuming and wastes reagents

Although there is also a multiplex PCR method for detecting multiple serum-specific genes, the reaction system has many primers, and the primers are prone to interference and mismatch, resulting in low detection accuracy and sensitivity

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