Escherichia coli O1, O2, O18 and O78 serotype detection kit and detection method thereof

Abstract

The invention belongs to the technical field of biology detection and relates to an escherichia coli O1, O2, O18 and O78 serotype detection kit and a detection method thereof. Forward primers of a primer group of the kit are highly conservative escherichia coli O antigen combined relative gene sequences, and backward primers are four serotype specific primers designed on the basis of four O1, O2, O18 and O78 serotype escherichia coli O antigen synthesis relative genes. The detection kit containing the primer group and the detection method of the detection kit have the advantages of being fast, sensitive, specific, low in cost, easy to operate and capable of well overcoming the shortcomings in the current escherichia coli O1, O2, O18 and O78 serotype detection method, can meet the requirements for the current escherichia coli O1, O2, O18 and O78 serotype detection, can be popularized and used easily in a wide range and have wide market prospect and large economic benefit.

Description

[0001] technical field [0002] The invention belongs to the technical field of biological detection, and in particular relates to a detection kit for Escherichia coli O1, O2, O18 and O78 serotypes and a detection method thereof. Background technique [0003] Escherichia coli is a common name for Escherichia coli (Escherichia coli, E.coli), a member of the family Enterobacteriaceae and the genus Escherichia, first discovered in 1885 by German bacteriologist Theodor Escherich Escherichia coli was isolated from infant feces, hence the name Escherichia coli. There are many types of Escherichia coli in nature, and the vast majority of non-pathogenic Escherichia coli are the main members of the normal flora in the intestinal tract of humans and animals, and have positive significance for the maintenance of normal physiological functions of the intestinal tract. However, some special serotypes are pathogenic and can cause diarrhea, urinary tract infection, pneumonia, sepsis, endoc...

AI Technical Summary

Problems solved by technology

However, previous reports all used the single-plex PCR method, which can only detect one serotype at a time, which is time-consuming and wastes reagents

Although there is also a multiplex PCR method for detecting multiple serum-specific genes, the reaction system has many primers, and the primers are prone to interference and mismatch, resulting in low detection accuracy and sensitivity

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