Bacillus pumilus JY2 and applications thereof

A Bacillus pumilus, JY2 technology, applied in applications, bacteria, enzymes, etc., can solve the problems of polluting the air environment, affecting metabolism, waste of resources, etc., and achieve the effects of rapid growth, reduced crude fiber content, and short fermentation cycle

Inactive Publication Date: 2014-10-08
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, less than 10% of straw is currently used as feed, and most of it is directly returned to the field or used as fuel, which not only causes a waste of resources, but also causes a large amount of smoke to be emitted into the air, seriously polluting the air en

Method used

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  • Bacillus pumilus JY2 and applications thereof
  • Bacillus pumilus JY2 and applications thereof
  • Bacillus pumilus JY2 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment one: bacillus pumilus ( Bacillus pumilus ) Isolation and screening of JY2 CGMCC No.9150

[0033] 1. Isolation: Bacillus pumilus JY2 of the present invention was isolated from a winter wheat field (N 72 ° 50' 70.01 "E 40 ° 26' 51.73 ") in Osh Racecourse, Kyrgyz Republic, randomly selected multiple depths in the farmland A soil sample of 3cm-20cm was mixed, put into a sterile container, transported to the laboratory in a car refrigerator, and a strain of Bacillus pumilus was isolated by the plate dilution coating method, numbered JY2.

[0034] Separation steps: According to the gradient dilution method, weigh 10g of soil samples in 90mL sterile physiological saline, and perform gradient dilution after activation at 30°C for 30min. Select 10 -2 、10 -3 、10 -4 The diluted solution was spread on the LB agar medium plate, and each treatment was replicated three times, and cultured at 37°C. After the colonies grow out, pick colonies of different shapes, sizes, ...

Embodiment 2

[0041] Embodiment two: bacillus pumilus ( Bacillus pumilus ) Sequence determination and phylogenetic analysis of JY2 CGMCC No. 9150

[0042] 1. DNA extraction: JY2 genomic DNA was extracted by CTAB / NaCl method. The strain was inoculated in LB medium (peptone 10g, yeast extract / powder 5g, NaCl 10g, agar 17g, distilled water 1000mL natural pH, sterilized at 121°C for 20 minutes.), 37°C shaking culture overnight. Take 1.5ml culture and centrifuge at 12000rpm for 2min. Add 567 ul of TE buffer to the precipitate, pipette repeatedly to resuspend it, add 30 ul of 10% SDS and 15 ul of proteinase K, mix well, and incubate at 37°C for 1 h.

[0043] Add 100ul 5mol / L NaCl, mix well, then add 80ul CTAB / NaCl solution, mix well, and then incubate at 65°C for 10min. Add an equal volume of phenol / chloroform / isoamyl alcohol and mix well, centrifuge for 4-5min, transfer the supernatant to a new tube, add 0.6-0.8 times the volume of isopropanol, mix gently until the DNA precipitates, Can be...

Embodiment 3

[0051] Embodiment three: bacillus pumilus ( Bacillus pumilus ) Antibacterial performance test of JY2 CGMCC No. 9150

[0052]Escherichia coli 1565 and Staphylococcus aureus were used as indicator bacteria to test the antibacterial performance in vitro. Take 0.5 mL of Escherichia coli, Salmonella and Bacillus pumilus JY2 bacterial fluid, and inoculate it in 5 mL common broth medium at the same time, and inoculate 0.5 mL of Escherichia coli and Salmonella bacterial fluid in the same common broth culture medium respectively. cultured as a control group. After shaking culture at 37°C for 18 hours, the CFU method was counted by serial dilution. The results showed that after JY2 was co-cultivated with Escherichia coli, the number of viable bacteria was 16.00% lower than that of single-inoculation Escherichia coli; after JY2 was co-cultured with Salmonella, the number of viable bacteria was 11.91% lower than that of single-inoculation Salmonella. Therefore, Bacillus pumilus (Baci...

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Abstract

The invention discloses Bacillus pumilus JY2 with the preservation number of CGMCC NO. 9150. The Bacillus pumilus JY2 is sampled from a winter wheat field (N 72 degrees 50 minutes 70.01 seconds E40 degrees 26 minutes 51.73 seconds) in an Osh race course of Kyrgyz Republic and is subjected to separating, screening and physiological and biochemical identification. The Bacillus pumilus JY2 with the preservation number of CGMCC NO. 9150, provided by the invention, has a good application effect on inhibiting pathogenic escherichia coli and salmonella, has a remarkable technical effect on applications in feeding microbioecologics and has broad application prospect in feed additive industries.

Description

technical field [0001] The invention relates to a microbial strain and its application field, specifically, the invention relates to a Bacillus pumilus and the technical field of its application. Background technique [0002] Crop straw is an important biological resource in the crop production system, and it is also the fourth largest energy source in the world after coal, oil and natural gas. According to incomplete estimates, nearly 20-30×10 8 t straw, while the annual output of crop straw in my country reaches 5.560×10 8 t, ranks first in the world, and is dominated by corn, wheat and rice straw, accounting for 80% of the total straw production. As it contains more than half of the energy accumulated by plant photosynthesis, straw is rich in raw sugar, crude protein and other ingredients that are beneficial to livestock and poultry. As a non-competitive feed resource, as long as it can be processed and modulated reasonably to improve its It is used to feed livestock a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12N9/52C12N9/42C12N9/16C12N9/20C12R1/225A23K10/18
Inventor 王宁包慧芳侯敏崔卫东王炜
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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